Removal of multiple electron acceptors by pilot-scale, two-stage membrane biofilm reactors

Zhao, He; Ontiveros‐Valencia, Aura; Tang, Youneng; Kim, Bi O.; VanGinkel, Steven; Friese, David; Overstreet, Ryan; Smith, Jennifer; Evans, Patrick; Krajmalnik‐Brown, Rosa; Rittmann, Bruce E.

doi:10.1016/j.watres.2014.01.047

Cited by 46 publications

(26 citation statements)

References 40 publications

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“…21,23 In the lead MBfRs, the NO3 -+ O2 flux was greater than ~ 0.34 g H2/m 2 -212 day 23 (Table 2), which completely suppressed SO4 2-reduction and is consistent with the 213 bench-scale results of Ontiveros-Valencia et al 45 and modeling work by Tang et al 46 …”

supporting

confidence: 75%

“…(Table 2). pyrosequencing is similar to the SRB trend noted by Zhao et al 23 using qPCR; however, 274 the qPCR study found that SRB had become the largest primary group in Condition 4,…”

supporting

confidence: 74%

“…Our work is complementary to the work reported by Zhao et al 23 , and both studies are 170 built on the field demonstration described by Evans et al 21 TTGACGGGGGCCCGCAC-3') and 1492R (5'TACCTTGTTACGACTT-3'). 33 We 182 processed the raw data using QIIME 1.7.0 suite 34 …”

supporting

confidence: 63%

“…While Zhao et al 23 used qPCR to provide an 129 analysis of community structure according to the primary respiratory groups, our findings 130 discriminate among conditions significantly altering the community structure, making the 131 biofilm more diverse and causing shifts within and outside the primary microbial groups.…”

mentioning

confidence: 88%

“…21 and Zhao et al 23 In brief, the two-stage MBfR was composed of two 500-gallon shows the side reactors with their connections for water and H2.…”

mentioning

confidence: 99%

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Pyrosequencing Analysis Yields Comprehensive Assessment of Microbial Communities in Pilot-Scale Two-Stage Membrane Biofilm Reactors

Ontiveros‐Valencia

Tang

Zhao

et al. 2014

Environ. Sci. Technol.

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24We studied the microbial community structure of pilot two-stage Membrane Biofilm 25Reactors (MBfRs) designed to reduce nitrate (NO3 -) and perchlorate (ClO4 -) in 26 contaminated groundwater. The groundwater also contained oxygen (O2) and sulfate 27(SO4 2-), which became important electron sinks that affected the NO3 -and ClO4 -removal 28 rates. Using pyrosequencing, we elucidated how important phylotypes of each "primary"

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supporting

confidence: 75%

“…(Table 2). pyrosequencing is similar to the SRB trend noted by Zhao et al 23 using qPCR; however, 274 the qPCR study found that SRB had become the largest primary group in Condition 4,…”

supporting

confidence: 74%

supporting

confidence: 63%

mentioning

confidence: 88%

“…21 and Zhao et al 23 In brief, the two-stage MBfR was composed of two 500-gallon shows the side reactors with their connections for water and H2.…”

mentioning

confidence: 99%

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Pyrosequencing Analysis Yields Comprehensive Assessment of Microbial Communities in Pilot-Scale Two-Stage Membrane Biofilm Reactors

Ontiveros‐Valencia

Tang

Zhao

et al. 2014

Environ. Sci. Technol.

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Assessing microbial competition in a hydrogen‐based membrane biofilm reactor (MBfR) using multidimensional modeling

Martin

Picioreanu

Nerenberg

2015

Biotech & Bioengineering

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The membrane biofilm reactor (MBfR) is a novel technology that safely delivers hydrogen to the base of a denitrifying biofilm via gas-supplying membranes. While hydrogen is an effective electron donor for denitrifying bacteria (DNB), it also supports sulfate-reducing bacteria (SRB) and methanogens (MET), which consume hydrogen and create undesirable by-products. SRB and MET are only competitive for hydrogen when local nitrate concentrations are low, therefore SRB and MET primarily grow near the base of the biofilm. In an MBfR, hydrogen concentrations are greatest at the base of the biofilm, making SRB and MET more likely to proliferate in an MBfR system than a conventional biofilm reactor. Modeling results showed that because of this, control of the hydrogen concentration via the intramembrane pressure was a key tool for limiting SRB and MET development. Another means is biofilm management, which supported both sloughing and erosive detachment. For the conditions simulated, maintaining thinner biofilms promoted higher denitrification fluxes and limited the presence of SRB and MET. The 2-d modeling showed that periodic biofilm sloughing helped control slow-growing SRB and MET. Moreover, the rough (non-flat) membrane assembly in the 2-d model provided a special niche for SRB and MET that was not represented in the 1-d model. This study compared 1-d and 2-d biofilm model applicability for simulating competition in counter-diffusional biofilms. Although more computationally expensive, the 2-d model captured important mechanisms unseen in the 1-d model.

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Concurrent microbial reduction of high concentrations of nitrate and perchlorate in an ion exchange membrane bioreactor

Fox

Bruner

Oren

et al. 2016

Biotech & Bioengineering

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We investigated effective simultaneous removal of high loads of nitrate and perchlorate from synthetic groundwater using an ion exchange membrane bioreactor (IEMB). The aim of this research was to characterize both transport aspects and biodegradation mechanisms involved in the treatment process of high loads of the two anions. Biodegradation process was proven to be efficient with over 99% efficiency of both perchlorate and nitrate, regardless of their load. The maximum biodegradation rates were 18.3 (mmol m(-2) h(-1) ) and 5.5 (mmol m(-2) h(-1) ) for nitrate and perchlorate, respectively. The presence of a biofilm on the bio-side of the membrane only slightly increased the nitrate and perchlorate transmembrane flux as compared to the measured flux during a Donnan dialysis experiment where there is no biodegradation of perchlorate and nitrate in the bio-compartment. The nitrate flux in presence of a biofilm was 18.3 (±1.9) (mmole m(-2) h(-1) ), while without the biofilm, the flux was 16.9 (±1.5) (mmole m(-2) h(-1) ) for the same feed inlet nitrate concentration of 4 mM. The perchlorate transmembrane flux increased similarly by an average of 5%. Samples of membrane biofilm and suspended bacteria from the bio-reactor were analyzed for diversity and abundance of the perchlorate and nitrate reducing bacteria. Klebsiella oxytoca, known as a glycerol fermenter, accounted for 70% of the suspended bacteria. In contrast, perchlorate and nitrate reducing bacteria predominated in the biofilm present on the membrane. These results are consistent with our proposed two stage biodegradation mechanism where glycerol is first fermented in the suspended phase of the bio-reactor and the fermentation products drive perchlorate and nitrate bio-reduction in the biofilm attached to the membrane. These results suggest that the niche exclusion of microbial populations in between the reactor and membrane is controlled by the fluxes of the electron donors and acceptors. Such a mechanism has important implications for controlling the bio-reduction reaction in the IEMB when using glycerol as a carbon source and allowing treating a complex contamination of high concentrations of perchlorate and nitrating in groundwater and successfully biodegrading them to non-hazardous components. Biotechnol. Bioeng. 2016;113: 1881-1891. © 2016 Wiley Periodicals, Inc.

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Removal of multiple electron acceptors by pilot-scale, two-stage membrane biofilm reactors

Cited by 46 publications

References 40 publications

Pyrosequencing Analysis Yields Comprehensive Assessment of Microbial Communities in Pilot-Scale Two-Stage Membrane Biofilm Reactors

Pyrosequencing Analysis Yields Comprehensive Assessment of Microbial Communities in Pilot-Scale Two-Stage Membrane Biofilm Reactors

Assessing microbial competition in a hydrogen‐based membrane biofilm reactor (MBfR) using multidimensional modeling

Concurrent microbial reduction of high concentrations of nitrate and perchlorate in an ion exchange membrane bioreactor

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