Abstract:IntroductionStudies with mesenchymal stem cells (MSCs) are increasing due to their immunomodulatory, anti-inflammatory and tissue regenerative properties. However, there is still no agreement about the best source of equine MSCs for a bank for allogeneic therapy. The aim of this study was to evaluate the cell culture and immunophenotypic characteristics and differentiation potential of equine MSCs from bone marrow (BM-MSCs), adipose tissue (AT-MSCs) and umbilical cord (UC-MSCs) under identical in vitro conditi… Show more
“…However, recent studies have shown that the differentiation potential of bone marrow progenitor cells and of the adipose tissue is similar (BARBERINI et al, 2014), and that the initially observed differences would due to lack of adjustments in the differentiation inducer medium necessary to the AdMSCs (STRIOGA et al, 2012). It is also worth highlighting that there are no studies comparing the therapeutic effects of MSCs from different sources (adipose tissue x bone marrow) used in equine tendinitis therapy; thus, it is necessary to conduct further investigations about it, since there are signs that the adequate source selection may broaden the effects of the cellular therapy (GARVICAN et al, 2014).…”
Section: What Is the Best Source To Be Used Adipose Tissue Or Bone Mmentioning
confidence: 99%
“…Since then, many isolation and featuring studies have been conducted (CARVALHO et al, 2009(CARVALHO et al, , 2013bMAMBELLI et al, 2009;BARBERINI et al, 2014). A growing number of studies assessing the impact of stem cells from adipose tissue on large animals have been conducted.…”
Section: The Mesenchymal Stem Cells From Adipose Tissue (Admscs)mentioning
Tendinitis is an important high-relapse-rate disease, which compromises equine performance and may result in early athletic life end to affected animals. Many therapies have been set to treat equine tendinitis; however, just few result in improved relapse rates, quality of extracellular matrix (ECM) and increased biomechanical resistance of the treated tissue. Due to advances in the regenerative medicine, promising results were initially obtained through the implantation of mesenchymal stem cells (MSC) derived from the bone marrow in the equine tendon injury. Since then, many studies have been using MSCs from different sources for therapeutic means in equine. The adipose tissue has appeared as feasible MSC source. There are promising results involving equine tendinitis therapy using mesenchymal stem cells from adipose tissue (AdMSCs). Keywords: Tendon. Therapy. Stem cells. Regenerative medicine.
ResumoA tendinite é uma importante afecção com alta taxa de recidivas que compromete o desempenho de equinos e pode resultar no término precoce da vida atlética dos animais acometidos. Diversas terapias têm sido estabelecidas para o tratamento da tendinite equina, no entanto poucas resultam na melhora da taxa de recidiva, melhora da qualidade da matriz extracelular (MEC) e aumento da resistência biomecânica do tecido tratado. Com o advento da medicina regenerativa resultados promissores foram inicialmente obtidos com o implante de células tronco mesenquimais (CTMs) derivadas da medula óssea na lesão tendínea de equinos. Desde então, diversos estudos têm sido conduzidos utilizando CTMs de diferentes fontes para esta finalidade terapêutica em equinos. O tecido adiposo tem se mostrado como uma fonte viável de CTMs e também há resultados promissores envolvendo a terapia da tendinite equina utilizando as células tronco mesenquimais derivadas do tecido adiposo (AdCTMs). Neste artigo, dados relacionados ao uso das AdCTMs na terapia da tendinite equina serão revisados. Palavras-chave: Tendão. Terapia. Células tronco. Medicina regenerativa.
“…However, recent studies have shown that the differentiation potential of bone marrow progenitor cells and of the adipose tissue is similar (BARBERINI et al, 2014), and that the initially observed differences would due to lack of adjustments in the differentiation inducer medium necessary to the AdMSCs (STRIOGA et al, 2012). It is also worth highlighting that there are no studies comparing the therapeutic effects of MSCs from different sources (adipose tissue x bone marrow) used in equine tendinitis therapy; thus, it is necessary to conduct further investigations about it, since there are signs that the adequate source selection may broaden the effects of the cellular therapy (GARVICAN et al, 2014).…”
Section: What Is the Best Source To Be Used Adipose Tissue Or Bone Mmentioning
confidence: 99%
“…Since then, many isolation and featuring studies have been conducted (CARVALHO et al, 2009(CARVALHO et al, , 2013bMAMBELLI et al, 2009;BARBERINI et al, 2014). A growing number of studies assessing the impact of stem cells from adipose tissue on large animals have been conducted.…”
Section: The Mesenchymal Stem Cells From Adipose Tissue (Admscs)mentioning
Tendinitis is an important high-relapse-rate disease, which compromises equine performance and may result in early athletic life end to affected animals. Many therapies have been set to treat equine tendinitis; however, just few result in improved relapse rates, quality of extracellular matrix (ECM) and increased biomechanical resistance of the treated tissue. Due to advances in the regenerative medicine, promising results were initially obtained through the implantation of mesenchymal stem cells (MSC) derived from the bone marrow in the equine tendon injury. Since then, many studies have been using MSCs from different sources for therapeutic means in equine. The adipose tissue has appeared as feasible MSC source. There are promising results involving equine tendinitis therapy using mesenchymal stem cells from adipose tissue (AdMSCs). Keywords: Tendon. Therapy. Stem cells. Regenerative medicine.
ResumoA tendinite é uma importante afecção com alta taxa de recidivas que compromete o desempenho de equinos e pode resultar no término precoce da vida atlética dos animais acometidos. Diversas terapias têm sido estabelecidas para o tratamento da tendinite equina, no entanto poucas resultam na melhora da taxa de recidiva, melhora da qualidade da matriz extracelular (MEC) e aumento da resistência biomecânica do tecido tratado. Com o advento da medicina regenerativa resultados promissores foram inicialmente obtidos com o implante de células tronco mesenquimais (CTMs) derivadas da medula óssea na lesão tendínea de equinos. Desde então, diversos estudos têm sido conduzidos utilizando CTMs de diferentes fontes para esta finalidade terapêutica em equinos. O tecido adiposo tem se mostrado como uma fonte viável de CTMs e também há resultados promissores envolvendo a terapia da tendinite equina utilizando as células tronco mesenquimais derivadas do tecido adiposo (AdCTMs). Neste artigo, dados relacionados ao uso das AdCTMs na terapia da tendinite equina serão revisados. Palavras-chave: Tendão. Terapia. Células tronco. Medicina regenerativa.
“…The expression of CD markers gets changed during the subsequent cell passages in several species and also due to laboratory procedures, biological differences between the donors because of species, age, gender and site of collection [5,6].…”
ADMSCs were cultured upto passage 2 (P 2 ). Characterization was done by using transcription factors namely Oct4 and Sox2 at P 0 level and was demonstrated by immunofluroscence technique. Immunophenotyping of ovine ADMSCs was done by using CD34 and CD44 monoclonal antibodies at P 0 and P 1 levels by using flow cytometry. At P0 level, 30.14 per cent ADMSCs were positive to CD44 and 13.83 per cent were positive for CD34 antibodies. At P 1 level, 40 per cent and 7.13 per cent cells were positive for CD44 and CD34 respectively. To conclude, Ovine ADMSCs were identified by Oct4 and Sox2 at P 0 level and CD44 positive cells were more at P 0 and P 1 level.
“…The literature offers conflicting data regarding the risk of spontaneous transformation of mesenchymal stem cells during іn vitro culture (2)(3)(4)16). Therefore, investigations of the biological properties of mesenchymal stem cells from equine bone marrow and foal umbilical cords as well as their cytogenetic analysis are highly relevant and timely tasks (13).…”
Introduction: The objective of the study was immunophenotypic and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture. Material and Methods: The mesenchymal stem cells were obtained from equine bone marrow of three horses and from foal umbilical cords of six foals. The cells were cultured in CO2 incubators by standard procedure. Quantitative abnormalities of chromosomes, i.e. aneuploidy and polyploidy, and structural aberrations, i.e. breaks in chromosomes and chromatid, were taken into account during the study. Results: The results of cytogenetic analysis of equine bone marrow mesenchymal stem cells at the third and fourth passages indicated that the level of karyotype variability of these cells corresponded to the spontaneous level of karyotype variability typical of the peripheral blood lymphocytes of this species. Equine bone marrow contained several clones of stem cells that differed in the expression of specific nuclear markers characteristic of proliferating cells. Conclusion: Mesenchymal stem cells from foal umbilical cords during in vitro cultivation are characterised by quantitative abnormalities of the chromosomal apparatus.
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