Third trimester NG2-positive amniotic fluid cells are effective in improving repair in spinal cord injury

Bottai, Daniele; Scesa, Giuseppe; Cigognini, Daniela; Adami, Raffaella; Nicora, E.; Abrignani, Sergio; Giulio, Anna Maria Di; Gorio, Alfredo

doi:10.1016/j.expneurol.2014.01.015

Cited by 29 publications

(16 citation statements)

References 43 publications

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“…The main outcome of these paper was that MSCs exert their effects by means of antioxidant and neuroprotective activity. Similar results were described in a mouse model of SCI where systemic treatment with Amniotic Fluid Stem cells (AFCs) was able to induce some recovery of motor function and partial spinal cord tissue preservation through an anti inflammatory mechanism which involved the production of the hepatocyte growth factor (Bottai et al, 2014 ).…”

Section: Different Types Of Stem Cells Used For the Treatment Of Neursupporting

confidence: 66%

Stem cell transplantation in neurological diseases: improving effectiveness in animal models

Adami¹,

Scesa²,

Bottai³

2014

Front. Cell Dev. Bio.

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Neurological diseases afflict a growing proportion of the human population. There are two reasons for this: first, the average age of the population (especially in the industrialized world) is increasing, and second, the diagnostic tools to detect these pathologies are now more sophisticated and can be used on a higher percentage of the population. In many cases, neurological disease has a pharmacological treatment which, as in the case of Alzheimer's disease, Parkinson's disease, Epilepsy, and Multiple Sclerosis can reduce the symptoms and slow down the course of the disease but cannot reverse its effects or heal the patient. In the last two decades the transplantation approach, by means of stem cells of different origin, has been suggested for the treatment of neurological diseases. The choice of slightly different animal models and the differences in methods of stem cell preparation make it difficult to compare the results of transplantation experiments. Moreover, the translation of these results into clinical trials with human subjects is difficult and has so far met with little success. This review seeks to discuss the reasons for these difficulties by considering the differences between human and animal cells (including isolation, handling and transplantation) and between the human disease model and the animal disease model.

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Section: Different Types Of Stem Cells Used For the Treatment Of Neursupporting

confidence: 66%

Stem cell transplantation in neurological diseases: improving effectiveness in animal models

Adami¹,

Scesa²,

Bottai³

2014

Front. Cell Dev. Bio.

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“…*P < 0.05, **P < 0.01, ***P < 0.001. belong to the most undifferentiated portion of amniotic fluid cells displaying pluripotency molecules, such as NANOG, SSEA-3, TRA-1-60, and TRA-1-81, and higher expression of organ-specific genes [22]. Accordingly, we found that first trimester-AFS cells express NG2 and CD34, two molecules normally expressed by undifferentiated SC subsets and embryonic SC-derived MSCs with enhanced self-renewal, higher colony forming unit efficiency, and long-term proliferative capacity [46,47].…”

Section: Cd45mentioning

confidence: 74%

Immune Regulatory Properties of CD117^pos Amniotic Fluid Stem Cells Vary According to Gestational Age

Trapani

Bassi²,

Fontana³

et al. 2015

Stem Cells and Development

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Amniotic Fluid Stem (AFS) cells are broadly multipotent fetal stem cells derived from the positive selection and ex vivo expansion of amniotic fluid CD117/c-kit pos cells. Considering the differentiation potential in vitro toward cell lineages belonging to the three germ layers, AFS cells have raised great interest as a new therapeutic tool, but their immune properties still need to be assessed. We analyzed the in vitro immunological properties of AFS cells from different gestational age in coculture with T, B, and natural killer (NK) cells. Nonactivated (resting) first trimester-AFS cells showed lower expression of HLA class-I molecules and NK-activating ligands than second and third trimester-AFS cells, whose features were associated with lower sensitivity to NK cell-mediated lysis. Nevertheless, inflammatory priming with interferon gamma (IFN-g) and tumor necrosis factor alpha (TNF-a) enhanced resistance of all AFS cell types to NK cytotoxicity. AFS cells modulated lymphocyte proliferation in a different manner according to gestational age: first trimester-AFS cells significantly inhibited T and NK cell proliferation, while second and third trimester-AFS cells were less efficient. In addition, only inflammatory-primed second trimester-AFS cells could suppress B cell proliferation, which was not affected by the first and third trimester-AFS cells. Indolamine 2,3 dioxygenase pathway was significantly involved only in T cell suppression mediated by second and third trimester-AFS cells. Overall, this study shows a number of significant quantitative differences among AFS cells of different gestational age that have to be considered in view of their clinical application.

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“…Very few groups have investigated the possibility of isolating stem cells from full-term pregnancy with none from murine has been reported (Bottai et al, 2014(Bottai et al, , 2012Fernandes et al, 2012;Rossi et al, 2014;You et al, 2009You et al, , 2008. Here, we aimed to isolate and characterize stem cells isolated from AF of full-term pregnant rats.…”

Section: Discussionmentioning

confidence: 92%

Rat full term amniotic fluid harbors highly potent stem cells

Mun-Fun

Ferdaos

Hamzah

et al. 2015

Research in Veterinary Science

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Third trimester NG2-positive amniotic fluid cells are effective in improving repair in spinal cord injury

Cited by 29 publications

References 43 publications

Stem cell transplantation in neurological diseases: improving effectiveness in animal models

Stem cell transplantation in neurological diseases: improving effectiveness in animal models

Immune Regulatory Properties of CD117^pos Amniotic Fluid Stem Cells Vary According to Gestational Age

Rat full term amniotic fluid harbors highly potent stem cells

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Third trimester NG2-positive amniotic fluid cells are effective in improving repair in spinal cord injury

Cited by 29 publications

References 43 publications

Stem cell transplantation in neurological diseases: improving effectiveness in animal models

Stem cell transplantation in neurological diseases: improving effectiveness in animal models

Immune Regulatory Properties of CD117pos Amniotic Fluid Stem Cells Vary According to Gestational Age

Rat full term amniotic fluid harbors highly potent stem cells

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Immune Regulatory Properties of CD117^pos Amniotic Fluid Stem Cells Vary According to Gestational Age