Effect of Sericin Supplementation During <i>In Vitro</i> Maturation on the Maturation, Fertilization and Development of Porcine Oocytes

K, L T; Namula, Zhao; Luu, V. V.; Sato, Yoko; Taniguchi, Masayasu; Isobe, Takeshi; Kikuchi, Kazuhiro; Otoi, Takeshige

doi:10.1111/rda.12274

Cited by 15 publications

(12 citation statements)

References 11 publications

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“…Moreover, sericin decreases the level of reactive oxygen species in cultures by preventing H 2 O 2 -induced oxidative stress , and the mechanism of this antioxidant action is the chelation of trace elements stemming from the remarkably high content of hydroxy amino acids in sericin (Kato et al, 1998). In a previous study, we reported that supplementation with 1.0% sericin during maturation culture improved the meiotic competence of porcine oocytes and the quality of blastocysts as determined by the DNA fragmentation index (Do et al, 2014). Moreover, we confirmed that sericin can prevent oxidative stress during bovine embryo culture, resulting in the improvement of embryo quality and increased embryonic development (Isobe et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Yasmin

Otoi

Setiadi

et al. 2015

Acta Veterinaria Hungarica

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Sericin is a water-soluble component of silk and has been used as a biomaterial due to its antibacterial and ultraviolet radiation-resistant properties. This study was designed to evaluate the effect of sericin supplementation in a maturation medium on the meiotic competence and fertilisability of sheep oocytes. Cumulus-oocyte complexes (COCs) were cultured in TCM199 supplemented with sericin at various concentrations of 0 (control), 0.1, 0.25 and 0.5%, either with or without bovine serum albumin (BSA). When the COCs were matured without BSA, the supplementation of 0.1% sericin significantly increased the rates of maturation to metaphase II and the total fertilisation of oocytes compared with the other concentrations of sericin. When the COCs were matured with BSA, the beneficial effects of 0.1% sericin supplementation on the maturation and fertilisation of oocytes were not observed. Our findings indicate that supplementation with 0.1% sericin during maturation culture may improve the nuclear maturation and fertilisability of sheep oocytes. Moreover, it may be possible to replace BSA with sericin in chemically defined media without the risk of disease transmission.

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Section: Discussionmentioning

confidence: 99%

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Yasmin

Otoi

Setiadi

et al. 2015

Acta Veterinaria Hungarica

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“…Our findings demonstrate for the first time that sericin supplementation significantly improves the developmental competence of HS embryos and could contribute to the production of bovine IVP embryos with high viability in terms of thermotolerance. Sericin has multifunctional properties, including not only antioxidant effects (Isobe et al, ; Takechi, Wada, Fukuda, Harada, & Takamura, ) but also stimulation of cell proliferation (Terada, Nishimura, Sasaki, Yamada, & Miki, ), improvement of cryotolerance (Miyamoto et al, , ; Ohnishi et al, ), and progression of oocyte maturation (Do et al, ), in addition to its use as a substitute for FBS on cell culture (Hosoe et al, ; Terada, Sasaki, Yanagihara, & Yamada, ). Therefore, although detailed mechanism is not clear, these properties of sericin may synergistically affect to protect embryos from heat shock.…”

Section: Discussionmentioning

confidence: 99%

“…Sericin has multifunctional properties, including not only antioxidant effects (Isobe et al, 2012;Takechi, Wada, Fukuda, Harada, & Takamura, 2014) but also stimulation of cell proliferation (Terada, Nishimura, Sasaki, Yamada, & Miki, 2002), improvement of cryotolerance (Miyamoto et al, 2010(Miyamoto et al, , 2012Ohnishi et al, 2012), and progression of oocyte maturation (Do et al, 2014), in addition to its use as a substitute for FBS on cell culture (Hosoe et al, 2014;Terada, Sasaki, Yanagihara, & Yamada, 2005). Therefore, although detailed mechanism is not clear, these properties of sericin may synergistically affect to protect embryos from heat shock.…”

Section: Discussionmentioning

confidence: 99%

Sericin enhances the developmental competence of heat‐stressed bovine embryos

Khatun

Egashira

Sakatani

et al. 2018

Molecular Reproduction Devel

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We investigated the effects of sericin on the developmental competence of bovine embryos exposed to heat stress (HS). Putative zygotes were cultured with sericin and subjected to HS (40.5°C for 6 hr) on Day 2 or 7 followed by continuous culture at 38.5°C until Day 8. Day 2 HS significantly decreased blastocyst development on Day 8 as well as mitochondrial activity, and significantly increased the amount of intracellular reactive oxygen species and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL)-positive cells, whereas Day 7 HS only significantly decreased mitochondrial activity and increased the number of TUNEL-positive cells in Day 8 blastocysts. These detrimental effects were neutralized by sericin supplementation. Next, to investigate the potential production of blastocysts with high viability in terms of thermotolerance, embryos were cultured with sericin until Day 7, and then exposed to HS in the sericin-free medium. TUNEL-positive cell numbers were significantly lower in blastocysts produced by sericin culture than in control blastocysts. Transcript abundance for HSPA1A and BAX was significantly decreased but IFNT2 levels were increased in blastocysts produced by sericin culture. In conclusion, these findings demonstrate the anti-oxidative and anti-apoptotic activities of sericin, and the potential use of sericin to produce embryos with high viability in vitro.

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“…This probably because after oocytes were collected and subsequently matured in vitro, meiotic coil of microtubules reorganized so that the oocytes remained can resume meiosis (Do et al, 2014). Wiedemann et al (2013) stated that cooling process at 20°C or 4°C could lead to microtubule depolymerization.…”

Section: Level Of Oocyte Maturationmentioning

confidence: 99%

Utilization of Oocytes Collected from Preserved Ovarian for In Vitro Production of Cat Embryos

et al. 2018

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Preservation of ovarian tissue from severely injured or dead valuable animals has the potential to preserve female germ cells of animals. The ability to maturize and fertilize the oocytes from preserved ovary of endangered species will allow us to sustain genetic and global biodiversities. The aims of this study were to investigate the viability of oocytes collected from the preserved ovary and its potential utilization for the production of cat embryos followed by in vitro maturation and fertilization. Ovary was preserved immediately in phosphate buffer saline (PBS) at 4 o C for 24 or 48 hours. The quality and viability of oocytes after the maturation process were identified microscopically using aceto-orcein staining. Biological function of the oocytes was evaluated by using in vitro culture technique for the maturation and fertilization rate in CR1aa medium culture. The results showed that the percentage of oocytes collected from preserved ovary for 24 and 48 hours that remained at the stage of metaphase-II were 29.4% and 21.9% respectively. Fertilization rates produced in the IVF using oocytes collected from ovary preserved for 24 or 48 hours were significantly lower (30%) than that of unpreserved control (36.7%). In conclusion, female germ cells of cat ovary preserved at 4 o C in PBS for 2 days were still viable for in vitro fertilization and thus can be utilized for in vitro production of cat embryos. Information obtained can be used as a basis of knowledge of using a combination of physiological reagent and cold-based preservation technique in modern reproductive technology for animals.

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Effect of Sericin Supplementation During In Vitro Maturation on the Maturation, Fertilization and Development of Porcine Oocytes

Cited by 15 publications

References 11 publications

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Sericin enhances the developmental competence of heat‐stressed bovine embryos

Utilization of Oocytes Collected from Preserved Ovarian for In Vitro Production of Cat Embryos

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