Utilization of Oocytes Collected from Preserved Ovarian for In Vitro Production of Cat Embryos

Abstract

Preservation of ovarian tissue from severely injured or dead valuable animals has the potential to preserve female germ cells of animals. The ability to maturize and fertilize the oocytes from preserved ovary of endangered species will allow us to sustain genetic and global biodiversities. The aims of this study were to investigate the viability of oocytes collected from the preserved ovary and its potential utilization for the production of cat embryos followed by in vitro maturation and fertilization. Ovary was preserved immediately in phosphate buffer saline (PBS) at 4 o C for 24 or 48 hours. The quality and viability of oocytes after the maturation process were identified microscopically using aceto-orcein staining. Biological function of the oocytes was evaluated by using in vitro culture technique for the maturation and fertilization rate in CR1aa medium culture. The results showed that the percentage of oocytes collected from preserved ovary for 24 and 48 hours that remained at the stage of metaphase-II were 29.4% and 21.9% respectively. Fertilization rates produced in the IVF using oocytes collected from ovary preserved for 24 or 48 hours were significantly lower (30%) than that of unpreserved control (36.7%). In conclusion, female germ cells of cat ovary preserved at 4 o C in PBS for 2 days were still viable for in vitro fertilization and thus can be utilized for in vitro production of cat embryos. Information obtained can be used as a basis of knowledge of using a combination of physiological reagent and cold-based preservation technique in modern reproductive technology for animals.

Kualitas dan Tingkat Maturasi Oosit Kucing Domestik dari Ovarium yang Disimpan dalam Waktu dan Media yang Berbeda

“…Oosit menurut Evecen et al (2009) persentase MII oosit kucing yang disimpan un secara nyata pada penyimpanan ovarium selama 48 jam. Demikian juga pada media PBS, Eriani et al (2018) melaporkan bahwa persentase oosit kucing yang mencapai MII menurun secara nyata pada penyimpanan ovarium dalam PBS selama 24 jam, sedangkan menurut Piras et al (2018) persentase MII pada oosit kucing menurun secara nyata pada penyimpanan ovarium dalam PBS selama 72 jam. Oleh karena itu, penelitian ini bertujuan untuk mengevaluasi kualitas dan tingkat maturasi oosit kucing domestik yang dikoleksi dari ovarium setelah disimpan pada periode waktu penyimpanan dan media yang berbeda.…”

“…Hasil penelitianPiras et al (2018) menunjukkan bahwa penyimpanan ovarium kucing selama 24 jam pada suhu 4 °C tidak memengaruhi morfologi dan integritas DNA folikel preantral. Didukung oleh penelitianEriani (2018) yang melaporkan bahwa preservasi ovarium pada suhu 4°C selama 48 jam dapat mempertahankan fungsi biologi oosit untuk mencapai tahap metaphase II (MII) secara in vitro fertiliztion (IVM). Persentase oosit yang mengalami degenerasi setelah maturasi in vitro pada penelitian ini juga ditemukan tidak berbeda antar kelompok perlakuan selama penyimanan 48 jam.…”