The Use of Platelet-Rich Fibrin in Combination With Biphasic Calcium Phosphate in the Treatment of Bone Defects: A Histologic and Histomorphometric Study

Abstract

BackgroundPlatelet-rich fibrin (PRF) is a leukocyte and platelet concentrate containing many growth factors. Its potential for hard tissue augmentation as a sole grafting material or in combination with other grafting materials has been investigated in many studies.ObjectiveThe aim of this histologic study was to evaluate the efficacy of PRF mixed with biphasic calcium phosphate (BCP) on bone regeneration in surgically created bone defects.MethodsDefects 5 mm in diameter were created in both tibias of 6 sheep. The defects were left empty or grafted with BCP, PRF, or BCP+PRF. Animals were killed at 10, 20, and 40 days. The specimens underwent histologic and histomorphometric analysis.ResultsNone of the groups displayed any signs of necrosis. Inflammation was observed in all groups at 10 days; 2 specimens of PRF+BCP and all empty defects showed inflammatory cell infiltration at 20 days. During the 40-day evaluation period, the PRF+BCP group showed the highest ratios of new bone. The other 3 groups showed statistically similar results. In the BCP and PRF+BCP groups, the residual graft ratios were decreased at consecutive time intervals. The difference between the 2 groups was not statistically significant during follow-up.ConclusionsThe current study revealed a histomorphometric increase in bone formation with the addition of PRF to BCP in surgically created defects in sheep tibia.

Nano-biphasic calcium phosphate/polyvinyl alcohol composites with enhanced bioactivity for bone repair via low-temperature three-dimensional printing and loading with platelet-rich fibrin

“…30,31 In this study, we fabricated an innovative 3D printed porous nano-BCP/PVA/PRF scaffold. There is little research on the combination of these three materials for bone repair applications, [32][33][34] let alone the 3D printing of this mixture. We hypothesized that the low-temperature 3D printing of BCP/ PVA/PRF scaffolds would preserve the biological activity of PRF and provide an innovative biomaterial for restoring segmental bone defects.…”

Platelet‐rich fibrin and collagen membrane in the preservation of the alveolar bone: Feasibility of the elemental inorganic composition and scanning electron microscopy analysis

“…The increasing interest in understanding the effects of platelet concentrate application on bone tissue is reflected in the number of recent studies that have examined this topic (Al‐Hamed et al, ; Canellas et al, ; Cortese et al, ; Isobe et al, ; Panda et al, ). Although there have been studies that have used models of bone lesions in animals to clarify the effects of these platelet concentrates at different levels (Acar et al, ; Bölükbaşı, Yeniyolm, Tekkesinm, & Altunatmaz, ; Du et al, ; Ocak et al, ), there are also clinical studies that focus on radiological (Cortese et al, ; Park et al, ) and tomographic analyses (Lim et al, ; Montanari, Callea, Yavuz, & Maglione, ; Öncü & Kaymaz, ) and its use in clinical settings (Barone et al, ; Panda et al, ; Sezgin, Uraz, Taner, & Çulhaoğlu, ), all of which seek to evaluate the effects of this therapy. Our clinical case study differs from these investigations as it evaluates the ultrastructure of neoformed bone tissue and the quantity of inorganic elements of HA present.…”

“…The findings of morphological and morphometric studies at the histological level have yielded inconclusive evidence of the overall effect of PRF use. Some of these studies revealed positive effects resulting from PRF application, such as improvements and increased bone formation in sheep (Bölükbaşı et al, ) and porcine (Yilmaz et al, ) tibia.…”

The Effect of Platelet Rich Fibrin on Angiogenesis During Periodontal Regeneration

“…PRF was cut and mixed with β-TCP particles to be applied inside the created defects then covered by PRF membrane; collagen membrane was placed to completely cover the augmented defect. This technique of PRF application was also carried out by Bölükbaşı et al; (10) to maximize the contact between the defect walls and PRF from inside and from outside in order to maximize the effect of PRF. Although Bölükbaşı et al; (10) did not cover their created defects with collagen membranes we utilized collagen membranes in both groups to standardize the study approach, so any histological changes observed in either group will be attributed mainly to the role of the applied material only.…”