Abstract:A TPCN1 gene–deficient mouse strain is used to show that two convergent working NAADP-dependent pathways with nonoverlapping activation and self-inactivation profiles for distinct NAADP concentrations drive acrosomal exocytosis, by which TPC1 is central for the pathway activated by low-micromolar NAADP concentrations.
“…However given the capacity of mouse embryos to compensate for loss of expression of important Ca 2+ signalling proteins by 'remodelling' other signalling pathways (Berridge et al, 2003), this does not exclude the possibility of important roles for TPCs during mammalian reproduction and embryogenesis. Indeed, a recent study that investigated the role of NAADP and TPCs in sperm biology found that NAADP could activate the acrosome reaction, whereas this response was blocked by treatment with Ned-19, as well as being absent in TPC1 knockout mice (Arndt et al, 2014). The study also found that NAADP binding sites and TPC1 showed co-localisation in the acrosomal region, providing further support for the idea that TPCs are NAADP-regulated Ca 2+ channels.…”
Section: Role Of Naadp and Tpcs During Fertilization And Embryogenesismentioning
confidence: 59%
“…The study also found that NAADP binding sites and TPC1 showed co-localisation in the acrosomal region, providing further support for the idea that TPCs are NAADP-regulated Ca 2+ channels. Interestingly, two narrow bellshaped dose-response curves were identified with maxima in either the nanomolar or low micromolar NAADP concentration range, with TPC1 being found to be responsible for activating the low affinity pathway by means of analysis of the TPC1 knockout (Arndt et al, 2014). This raises the question of whether the high affinity pathway involves TPC2, an issue that remains to be explored.…”
Section: Role Of Naadp and Tpcs During Fertilization And Embryogenesismentioning
“…However given the capacity of mouse embryos to compensate for loss of expression of important Ca 2+ signalling proteins by 'remodelling' other signalling pathways (Berridge et al, 2003), this does not exclude the possibility of important roles for TPCs during mammalian reproduction and embryogenesis. Indeed, a recent study that investigated the role of NAADP and TPCs in sperm biology found that NAADP could activate the acrosome reaction, whereas this response was blocked by treatment with Ned-19, as well as being absent in TPC1 knockout mice (Arndt et al, 2014). The study also found that NAADP binding sites and TPC1 showed co-localisation in the acrosomal region, providing further support for the idea that TPCs are NAADP-regulated Ca 2+ channels.…”
Section: Role Of Naadp and Tpcs During Fertilization And Embryogenesismentioning
confidence: 59%
“…The study also found that NAADP binding sites and TPC1 showed co-localisation in the acrosomal region, providing further support for the idea that TPCs are NAADP-regulated Ca 2+ channels. Interestingly, two narrow bellshaped dose-response curves were identified with maxima in either the nanomolar or low micromolar NAADP concentration range, with TPC1 being found to be responsible for activating the low affinity pathway by means of analysis of the TPC1 knockout (Arndt et al, 2014). This raises the question of whether the high affinity pathway involves TPC2, an issue that remains to be explored.…”
Section: Role Of Naadp and Tpcs During Fertilization And Embryogenesismentioning
“…Being a Na ϩ /H ϩ -exchanger, its loss may change both vesicular pH and Na ϩ concentration. These changes can secondarily affect other luminal ion concentrations like that of Ca 2ϩ , the release of which may be needed for vesicle fusion and trafficking (53)(54)(55). Although not quite reaching the pH of lysosomes, the luminal pH of acrosomes (pH ϳ5.3) (56, 57) is more acidic than that of the Golgi (estimated pH ϳ5.9 -6.3, depending on cell type) (58 -60).…”
Section: Loss Of Nhe8 Disrupts Acrosome Formation In Micementioning
“…The acrosome is characterized by the unique properties of sperm cells, and acrosomal enzyme contains various hydrolytic enzymes that allow sperm to break through the external barrier to the oocyte [1][2][3][4]. Sperm can release a variety of enzymes after the acrosome reaction to stimulate sperm cells to combine with egg cells.…”
Objective: Using correlation analysis between the sperm spontaneous acrosome reaction rate (SARR) and routine semen parameters in male infertility patients, we explored the value of the sperm spontaneous acrosome reaction in the evaluation of sperm functions.
Methods:The participants comprised of 219 male infertility patients who had visited the Center for Reproductive Medicine, Shandong University from October 2016 to March 2017. According to the sperm SARR, we classified the patients with infertility into the control group and the case group. According to the WHO human semen examination and processing laboratory manual, fifth edition, recommendations and standards. We obtained routine semen parameters including semen volume, sperm concentration, total motility, and progressive sperm rate, and we measured sperm motility rate and the proportion of sperm with normal morphology.Results: With regard to the total motility, progressive sperm rate, sperm survival rate and the proportion of sperm with normal morphology, there were statistically significant differences between the two groups (p<0.01), but the data concerning patients' age, semen volume, or sperm concentration showed no significant difference (p>0.05).
Conclusion:Sperm SARR in patients with infertility is closely related to routine semen parameters and plays a supplementary reference in male fertility evaluation.
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