“…Experimental techniques including microarray [12] and SILAC [17,18] have been used to identify targeted transcripts and proteins respectively, that are differentially expressed upon the exogenous addition of the miRNA of interest or anti-sense inhibitors to the miRNA [18]. Recently, biochemical approaches based on RISC immunoprecipitation (RIP), including RIP-Chip, PAR-CLIP, and HITS-CLIP, have been developed to directly identify targeted transcripts incorporated into the RISC complex [2,13,19-26]. These approaches usually identify transcripts containing seed matches (upwards of 75%) and experimental validation has been exceptionally high compared with bioinformatics approaches [13,27,28].…”