Hand-held high-resolution fluorescence imaging system for fluorescence-guided surgery of patient and cell-line pancreatic tumors growing orthotopically in nude mice

Abstract

Background In this study, we investigated the advantages for fluorescence-guided surgery (FGS) in mice of a portable hand-sized imaging system compared to a large chamber fluorescing imaging system or a long-working-distance fluorescence microscope. Methods Mouse models of human pancreatic cancer for FGS included (1) MiaPaCa-2-expressing green fluorescent protein (GFP), (2) BxPC3 labeled with anti-CEA antibody conjugated with Alexa 488, (3) patient-derived orhotopic xenograft (PDOX)™ labeled with anti-CA19-9 antibody conjugated with Alexa 488. Results Each device could clearly detect the primary MiaPaCa-2-GFP. tumor and any residual tumor after FGS. In the BxPC3 model labeled with Alexa 488-conjugated anti-CEA, each device could detect the primary tumor, but the MVX10 could not clearly detect the residual tumor remaining after FGS while the other devices could. In the PDOX™ model labeled with Alexa 488 conjugated with anti CA19-9, only the portable hand-held device could distinguish the residual tumor from the background, and complete resection of the residual tumor was achieved under fluorescence navigation. Conclusions The results described in the present report suggest the hand-held mobile imaging system can be able to be applied to the clinic for FGS due to its convenient size and high sensitivity and help make FGS widely-used.

Aging hair follicles rejuvenated by transplantation to a young subcutaneous environment

“…An "L" shape incision in the skin on the back of nude mice was made using fine scissors and the hair follicle was carefully inserted under the skin. On day-0, after obtaining images with the portable Dino-Lite microscope imager (AM4113TGFBW Dino-Lite Premier; AnMo Electronics Corporation, Taiwan), 29 the skin was closed with a 6-0 suture. The host young nude mice were approximately 4 weeks old, and aging host nude mice were approximately 7.5 months old.…”

Specific tumor labeling enhanced by polyethylene glycol linkage of near infrared dyes conjugated to a chimeric anti-carcinoembryonic antigen antibody in a nude mouse model of human pancreatic cancer

“…The cumulative effects of PEGylation were to increase dye-conjugated anti-CEA antibody half-life, favorably alter the biodistribution, and significantly increase tumor to background contrast. PEGylated dyes conjugated to tumor-specific antibodies should have an important impact in the development of fluorescence guided surgery of cancer, 1,[11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] as these dyes offer the possibility of high fidelity-targeted tumor and metastases labeling.…”

The role of mouse models in translational cancer research: present and future directions

“…28,29 Moreover, a fluorescently-labelled chimeric anti-CEA antibody was able to detect the primary tumor and the residual mass after the surgical resection in whole colon cancer PDOXs. 30 …”