Improved production of reducing sugars from rice husk and rice straw using bacterial cellulase and xylanase activated with hydroxyapatite nanoparticles

Dutta, Nalok; Mukhopadhyay, Arka; Dasgupta, Anjan Kr.; Chakrabarti, Krishanu

doi:10.1016/j.biortech.2013.12.016

Cited by 59 publications

(16 citation statements)

References 28 publications

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“…Considering few references about the direct relation between L. edodes xylanase and agricultural straws, this paper compared our results with those obtained using xylanase from other microorganisms. Similar to our results, previous studies also found that xylanase from some bacteria or fungus exhibited high hydrolytic efficiency toward agricultural straws ( Zilliox and Debeire, 1998 ; Zhang et al, 2011 ; Dutta et al, 2014 ). In addition, the dramatical ability of rLeXyn11A to hydrolyze agricultural straws may help to explain why significant xylanase activities were detected in L. edodes cultivation using agricultural straws ( Tsujiyama and Ueno, 2013 ; Elisashvili et al, 2015 ).…”

Section: Discussionsupporting

confidence: 92%

Expression of a Recombinant Lentinula edodes Xylanase by Pichia pastoris and Its Effects on Ruminal Fermentation and Microbial Community in in vitro Incubation of Agricultural Straws

Liu

et al. 2018

Front. Microbiol.

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Agricultural straws, such as rice straw, wheat straw, and corn straw, are produced abundantly every year but not utilized efficiently in China. An experiment was conducted to determine the effects of recombinant xylanase on ruminal fermentation and microbial community structure in in vitro incubation of these straws. The recombinant xylanase from Lentinula edodes (rLeXyn11A) was produced in Pichia pastoris. The optimal temperature and pH for rLeXyn11A were 40°C and 4.0, respectively. The rLeXyn11A featured resistance to high temperature and showed broad temperature adaptability (>50% of the maximum activity at 20–80°C). Supplemental rLeXyn11A enhanced the hydrolysis of three agricultural straws. After in vitro ruminal incubation, regardless of agricultural straws, the fiber digestibility, acetate concentration, total volatile fatty acids (VFAs) production, and fermentation liquid microbial protein were increased by rLeXyn11A. Supplemental rLeXyn11A increased the ammonia-N concentration for corn straw and rice straw. High throughput sequencing and real-time PCR data showed that the effects of rLeXyn11A on ruminal microbial community depended on the fermentation substrates. With rice straw, rLeXyn11A increased the relative abundance of fibrolytic bacteria including Firmicutes, Desulfovibrio, Ruminococcaceae and its some genus, and Fibrobacter succinogenes. With wheat straw, rLeXyn11A increased the relative abundance of Ruminococcus_1 and its three representative species F. succinogenes, Ruminococcus flavefaciens, Ruminococcus albus. With corn straw, the fibrolytic bacteria Firmicutes, Christensenellaceae_R_7_group, Saccharofermentans, and Desulfovibrio were increased by rLeXyn11A. This study demonstrates that rLeXyn11A could enhance in vitro ruminal digestion and fermentation of agricultural straws, showing the potential of rLeXyn11A for improving the utilization of agricultural straws in ruminants.

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Section: Discussionsupporting

confidence: 92%

Expression of a Recombinant Lentinula edodes Xylanase by Pichia pastoris and Its Effects on Ruminal Fermentation and Microbial Community in in vitro Incubation of Agricultural Straws

Liu

et al. 2018

Front. Microbiol.

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“…To investigate the cellulose-degrading ability, B. velezensis LC1 was cultured on CMC agar to determine cellulase activities by the dinitrosalicylic acid spectrophotometric (DNS) method for 6 days ( Fig. 1e-g) [20]. The cellulase activities of strain LC1 were then determined.…”

Section: Identification and Cellulose-degrading Potential Of Bacillusmentioning

confidence: 99%

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Lü

Zheng

et al. 2020

Biotechnol Biofuels

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Background: Bamboo, a lignocellulosic feedstock, is considered as a potentially excellent raw material and evaluated for lignocellulose degradation and bioethanol production, with a focus on using physical and chemical pre-treatment. However, studies reporting the biodegradation of bamboo lignocellulose using microbes such as bacteria and fungi are scarce. Results:In the present study, Bacillus velezensis LC1 was isolated from Cyrtotrachelus buqueti, in which the symbiotic bacteria exhibited lignocellulose degradation ability and cellulase activities. We performed genome sequencing of B. velezensis LC1, which has a 3929,782-bp ring chromosome and 46.5% GC content. The total gene length was 3,502,596 bp using gene prediction, and the GC contents were 47.29% and 40.04% in the gene and intergene regions, respectively. The genome contains 4018 coding DNA sequences, and all have been assigned predicted functions. Carbohydrate-active enzyme annotation identified 136 genes annotated to CAZy families, including GH, GTs, CEs, PLs, AAs and CBMs. Genes involved in lignocellulose degradation were identified. After a 6-day treatment, the bamboo shoot cellulose degradation efficiency reached 39.32%, and the hydrolysate was subjected to ethanol fermentation with Saccharomyces cerevisiae and Escherichia coli KO11, yielding 7.2 g/L of ethanol at 96 h. Conclusions:These findings provide an insight for B. velezensis strains in converting lignocellulose into ethanol. B. velezensis LC1, a symbiotic bacteria, can potentially degrade bamboo lignocellulose components and further transformation to ethanol, and expand the bamboo lignocellulosic bioethanol production.

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“…Hence, rice husk is considered for a wide range of applications. At present, the rice husk is being investigated for the preparation of combustible gases [3], adsorbents [4] and catalysts [5], activated carbon [6], chemical products (i.e., xylose [7] and furfural [8]), and various silicon products [9]. Moreover, rice husk has shown potential in the field of composite reinforcements [10,11].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Mechanical and Water Absorption Properties of Rice Husk-Derived Nano-SiO2 Reinforced PHBV Composites

et al. 2018

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Poly(3-hydroxybutyrate–co–3-hydroxyvalerate) (PHBV) is a biodegradable polymer and has several potential applications. Herein, we have used a rich biomass resource, rice husk, to obtain rice husk nano-SiO2 (RHNS) and prepared RHNS/PHBV composites by using hot-press molding. The results showed that the amorphous nature of spherical nano-SiO2 particles with an average diameter of 40–80 nm was obtained. The tensile strength and flexural strength of the RHNS/PHBV-3 composite reached up to 23.515 and 75.669 MPa, respectively, corresponding to an increase of 33.65% and 15.54% as compared to pure PHBV. The enhanced mechanical properties of the RHNS/PHBV composite can be attributed to the uniform dispersion and strong interfacial bonding of RHNS with the PHBV matrix. In addition, the water absorption rate of the RHNS/PHBV composite increased from 0.26% to 0.35% and the water swelling ratio followed the given order in different directions: thickness > width > length. Furthermore, the initial degradation temperature and residual rate of combustion at 700 °C of the composites increased with higher content of RHNS, which represents the enhanced thermal stability of RHNS/PHBV composites. In summary, RHNS served as an excellent reinforcement and RHNS/PHBV composites have shown promising properties for various potential applications.

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Improved production of reducing sugars from rice husk and rice straw using bacterial cellulase and xylanase activated with hydroxyapatite nanoparticles

Cited by 59 publications

References 28 publications

Expression of a Recombinant Lentinula edodes Xylanase by Pichia pastoris and Its Effects on Ruminal Fermentation and Microbial Community in in vitro Incubation of Agricultural Straws

Expression of a Recombinant Lentinula edodes Xylanase by Pichia pastoris and Its Effects on Ruminal Fermentation and Microbial Community in in vitro Incubation of Agricultural Straws

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Enhanced Mechanical and Water Absorption Properties of Rice Husk-Derived Nano-SiO2 Reinforced PHBV Composites

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