27-Hydroxycholesterol and 7alpha-hydroxycholesterol trigger a sequence of events leading to migration of CCR5-expressing Th1 lymphocytes

Kim, Sunmi; Kim, Boyoung; Lee, Sae-A; Eo, Seong-Kug; Yun, Yungdae; Kim, Chi-Dae; Kim, Koanhoi

doi:10.1016/j.taap.2013.12.007

Cited by 40 publications

(34 citation statements)

References 45 publications

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“…These results agree with those of our previous reports showing that 27OHChol is much more active than cholesterol itself in terms of expression of pro-inflammatory molecules, including tumor necrosis factor-a, CCL2, CCL3, CCL4, CD14, CD40, CD137, and Toll-like receptor 6 [10e12, 16,22,23]. The increased expression of CXCL8 in the presence of 27OHChol is in line with the study by Gargiulo et al [24].…”

Section: Discussionsupporting

confidence: 93%

“…We found increased transcription of the IL-8 gene in the arteries and elevated levels of secreted CXCL8 in sera of ApoE À/À mice, which were kept in a specific-pathogen-free facility, in comparison with wild-type mice after a high-cholesterol diet. These results indicate that a high-cholesterol milieu is sufficient for transcription of the IL-8 gene and secretion of CXCL8 in vivo, in addition to CCL2, CCL3, and CCL4 [11,22].…”

Section: Discussionmentioning

confidence: 79%

“…Because small amount of endotoxin causes expression of multiple pro-inflammatory chemokines, including CCL2, CCL3, CCL4, and CXCL8 in monocytes/macrophage lineage cells [8,11,22,26], it is important to exclude possible contribution of contaminating endotoxin to chemokine expression. The enhanced expression of CXCL8 in THP-1 cells was not due to endotoxin because cotreatment with polymyxin B, which prevented the biological effects of LPS, did not cause attenuation in gene transcription of IL-8 induced by 27OHChol.…”

Section: Discussionmentioning

confidence: 99%

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27-Oxygenated cholesterol induces expression of CXCL8 in macrophages via NF-κB and CD88

Kim

Lee

Kim

et al. 2015

Biochemical and Biophysical Research Communications

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

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27-Oxygenated cholesterol induces expression of CXCL8 in macrophages via NF-κB and CD88

Kim

Lee

Kim

et al. 2015

Biochemical and Biophysical Research Communications

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“…Treatment of THP-1 cells with 7αOHChol causes phosphorylation of Akt and ERK1/2 [13]. Therefore, we examined whether the PI3K and MEK were involved in 7αOHChol-induced expression of IL-8 (Fig 3A).…”

Section: Resultsmentioning

confidence: 99%

“…7βOHChol and 7K induce apoptosis at high concentrations [15, 16]. 7αOHChol upregulates surface levels of Toll-like receptor 6 and induces production of CCL2, CCL3 and CCL4 and thereby promotes migration of monocytic cells and T lymphocytes expressing CCR5 [13, 17, 18]. 7K and 7αOHChol induces differentiation of monocytes into macrophages and mature dendritic cell phenotype, respectively [19, 20].…”

Section: Discussionmentioning

confidence: 99%

7α-Hydroxycholesterol induces monocyte/macrophage cell expression of interleukin-8 via C5a receptor

Cho¹,

Son²,

Kim³

et al. 2017

PLoS ONE

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We investigated effects of 7-oxygenated cholesterol derivatives present in atherosclerotic lesions, 7α-hydroxycholesterol (7αOHChol), 7β-hydroxycholesterol (7βOHChol), and 7-ketocholesterol (7K), on IL-8 expression. Transcript levels of IL-8 and secretion of its corresponding gene product by monocytes/macrophages were enhanced by treatment with 7αOHChol and, to a lesser extent, 7K, but not by 7βOHChol. The 7-oxygenated cholesterol derivatives, however, did not change transcription of the IL-8 gene in vascular smooth muscle cells. 7αOHChol-induced IL-8 gene transcription was inhibited by cycloheximide and Akt1 downregulation, but not by OxPAPC. Expression of C5a receptor was upregulated after stimulation with 7αOHChol, but not with 7K and 7βOHChol, and a specific antagonist of C5a receptor inhibited 7αOHChol-induced IL-8 gene expression in a dose dependent manner. Pharmacological inhibitors of PI3K and MEK almost completely inhibited expression of both IL-8 and cell-surface C5a receptor induced by 7αOHChol. These results indicate that 7-oxygenated cholesterol derivatives have differential effects on monocyte/macrophage expression of IL-8 and C5a receptor and that C5a receptor is involved in 7αOHChol-induced IL-8 expression via PI3K and MEK.

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Cholesterol‐autoxidation metabolites in host defense against infectious diseases

Shi

Zhan

et al. 2023

Eur J Immunol

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Cholesterol plays essential roles in biological processes, including cell membrane stability and myelin formation. Cholesterol can be metabolized to oxysterols by enzymatic or nonenzymatic ways. Nonenzymatic cholesterol metabolites, also called cholesterol‐autoxidation metabolites, are formed dependent on the oxidation of reactive oxygen species (ROS) such as OH• or reactive nitrogen species, such as ONOO−. Cholesterol‐autoxidation metabolites are abundantly produced in diseases such as inflammatory bowel disease and atherosclerosis, which are associated with oxidative stress. Recent studies have shown that cholesterol‐autoxidation metabolites can further regulate the immune system. Here, we review the literature and summarize how cholesterol‐autoxidation metabolites, such as 25‐hydroxycholesterol (25‐OHC), 7α/β‐OHC, and 7‐ketocholesterol, deal with the occurrence and development of infectious diseases through pattern recognition receptors, inflammasomes, ROS production, nuclear receptors, G‐protein‐coupled receptor 183, and lipid availability. In addition, we include the research regarding the roles of these metabolites in COVID‐19 infection and discuss our viewpoints on the future research directions.

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27-Hydroxycholesterol and 7alpha-hydroxycholesterol trigger a sequence of events leading to migration of CCR5-expressing Th1 lymphocytes

Cited by 40 publications

References 45 publications

27-Oxygenated cholesterol induces expression of CXCL8 in macrophages via NF-κB and CD88

27-Oxygenated cholesterol induces expression of CXCL8 in macrophages via NF-κB and CD88

7α-Hydroxycholesterol induces monocyte/macrophage cell expression of interleukin-8 via C5a receptor

Cholesterol‐autoxidation metabolites in host defense against infectious diseases

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