27-Oxygenated cholesterol induces expression of CXCL8 in macrophages via NF-κB and CD88

Kim, Sunmi; Lee, Chung Won; Kim, Boyoung; Jung, Young-Suk; Eo, Seong-Kug; Park, Young Chul; Kim, Koanhoi

doi:10.1016/j.bbrc.2015.06.075

Cited by 16 publications

(10 citation statements)

References 32 publications

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“…The expression of IL8 was decreased when cells were treated with LY or AZD. Many studies have demonstrated that the most common upstream target of chemokines is RelA (13,52,53), and RelA can regulate IL8 (13,32). These results prompted us to investigate whether RelA could regulate IL8 in ESCC.…”

Section: Discussionmentioning

confidence: 99%

Maelstrom Directs Myeloid-Derived Suppressor Cells to Promote Esophageal Squamous Cell Carcinoma Progression via Activation of the Akt1/RelA/IL8 Signaling Pathway

Chen

Qin

et al. 2018

Cancer Immunology Research

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() is a novel cancer/testis-associated gene, which is not only expressed in the male testicular germ cells among human normal tissues, but is also aberrantly expressed in various cancer tissues. In our study, was characterized as a tumor-promoting gene and was significantly associated with esophageal squamous cell carcinoma (ESCC) recurrence and unfavorable prognosis. Kaplan-Meier analysis showed that patients with high expression had a shorter survival time. Functional experiments showed that promoted tumor cell growth and inhibited cell apoptosis. These results prompted us to investigate the factors affecting the tumorigenicity of Further experimentation demonstrated that enhanced the expression of phosphorylated Akt1, with subsequent phosphorylation of nuclear factor kappa B (NF-κB) subunit RelA in tumor cells, and chemoattracted myeloid-derived suppressor cells (MDSCs) by upregulating interleukin-8 (IL8) to accelerate tumor progression in the tumor microenvironment. We also found that TGFβ secreted by MDSCs could upregulate by inducing Smad2/Smad3 phosphorylation. In summary, this study revealed a mechanism by which could upregulate IL8 through Akt1/RelA to direct MDSCs homing into the tumor, suggesting that could be an attractive therapeutic target and a prognostic marker against ESCC. .

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Section: Discussionmentioning

confidence: 99%

Maelstrom Directs Myeloid-Derived Suppressor Cells to Promote Esophageal Squamous Cell Carcinoma Progression via Activation of the Akt1/RelA/IL8 Signaling Pathway

Chen

Qin

et al. 2018

Cancer Immunology Research

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“…In vitro studies, however, have found that 27-HC can also have pro-inflammatory effects on macrophages and other tissues (121)(122)(123)(124)(125), which contradicts our findings in Chapter 5. One possible explanation for these observations is that 27-HC also functions as of a selective estrogen receptor modulator.…”

Section: Modulating Intracellular Cholesterol Transport Influences Lycontrasting

confidence: 99%

The entanglement of NASH and atherosclerosis

Jeurissen¹

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“…IL-8 gene transcription induced by 7αOHChol, which is detectable at 24 h post-treatment, is sustained through 72 h of post-treatment. 27OHChol-induced IL-8 gene transcription persists up to 48 h and disappears by 72 h of post-treatment [12]. The oxysterol molecules also show differences in their minimal concentrations to induce IL-8 transcription.…”

Section: Discussionmentioning

confidence: 99%

“…After reverse-transcription of total RNAs for 1 h at 42°C with Moloney Murine Leukemia Virus reverse transcriptase, transcripts of the IL-8 gene were amplified by RT-PCR or assessed by real-time PCR using a LightCycler® 96 Real-Time PCR System (Roche, Germany) as described [12]. PCR was performed using Taq PCR Kit.…”

Section: Methodsmentioning

confidence: 99%

7α-Hydroxycholesterol induces monocyte/macrophage cell expression of interleukin-8 via C5a receptor

Cho¹,

Son²,

Kim³

et al. 2017

PLoS ONE

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We investigated effects of 7-oxygenated cholesterol derivatives present in atherosclerotic lesions, 7α-hydroxycholesterol (7αOHChol), 7β-hydroxycholesterol (7βOHChol), and 7-ketocholesterol (7K), on IL-8 expression. Transcript levels of IL-8 and secretion of its corresponding gene product by monocytes/macrophages were enhanced by treatment with 7αOHChol and, to a lesser extent, 7K, but not by 7βOHChol. The 7-oxygenated cholesterol derivatives, however, did not change transcription of the IL-8 gene in vascular smooth muscle cells. 7αOHChol-induced IL-8 gene transcription was inhibited by cycloheximide and Akt1 downregulation, but not by OxPAPC. Expression of C5a receptor was upregulated after stimulation with 7αOHChol, but not with 7K and 7βOHChol, and a specific antagonist of C5a receptor inhibited 7αOHChol-induced IL-8 gene expression in a dose dependent manner. Pharmacological inhibitors of PI3K and MEK almost completely inhibited expression of both IL-8 and cell-surface C5a receptor induced by 7αOHChol. These results indicate that 7-oxygenated cholesterol derivatives have differential effects on monocyte/macrophage expression of IL-8 and C5a receptor and that C5a receptor is involved in 7αOHChol-induced IL-8 expression via PI3K and MEK.

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27-Oxygenated cholesterol induces expression of CXCL8 in macrophages via NF-κB and CD88

Cited by 16 publications

References 32 publications

Maelstrom Directs Myeloid-Derived Suppressor Cells to Promote Esophageal Squamous Cell Carcinoma Progression via Activation of the Akt1/RelA/IL8 Signaling Pathway

Maelstrom Directs Myeloid-Derived Suppressor Cells to Promote Esophageal Squamous Cell Carcinoma Progression via Activation of the Akt1/RelA/IL8 Signaling Pathway

The entanglement of NASH and atherosclerosis

7α-Hydroxycholesterol induces monocyte/macrophage cell expression of interleukin-8 via C5a receptor

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