Significant prevalence of antibodies reacting with simian virus 40 mimotopes in sera from patients affected by glioblastoma multiforme

Mazzoni, Elisa; Gerosa, Massimo; Lupidi, Francesco; Corallini, Alfredo; Taronna, Angelo; D’Agostino, Antonio; Bovenzi, Massimo; Ruggeri, Giuseppina; Casali, Ferruccio; Rotondo, John Charles; Rezza, Giovanni; Barbanti‐Brodano, Giuseppe; Tognon, Mauro; Martini, Fernanda

doi:10.1093/neuonc/not217

Cited by 24 publications

(58 citation statements)

References 38 publications

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“…To verify the neutralization activity of SV40 immune sera from pregnant women, an inhibition test was performed (29). SV40 immune sera ( n = 4) from pregnant women and non-pregnant women ( n = 4) with an OD (range OD = 0.193–0.876) were challenged to inhibit the SV40 CPE in CV1-infected monolayers.…”

Section: Resultsmentioning

confidence: 99%

“…In order to verify which sera from pregnant women were BKPyV-positive, HAI assays were carried out, as described before (23, 29). Briefly, BKPyV viral working stocks, employed for HAI, were obtained in infected Vero cells, purchased from the ATCC.…”

Section: Methodsmentioning

confidence: 99%

“…BKPyV was employed as antigens in HA and HAI tests. HA and HAI titers for BKPyV were obtained as reported before (23, 29, 30). These assays allow the detection of serum Ab against the human polyomavirus BKPyV, which abolish its agglutination properties.…”

Section: Methodsmentioning

confidence: 99%

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Serum IgG Antibodies from Pregnant Women Reacting to Mimotopes of Simian Virus 40 Large T Antigen, the Viral Oncoprotein

et al. 2017

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Simian virus 40 (SV40) large T antigen (LT) coding sequences were revealed in different human samples, whereas SV40 antibodies (Ab) were detected in human sera of cancer patients and healthy individuals, although with a lower prevalence. Previous studies carried out by the neutralization assay gave a SV40 seroprevalence, in the general population, up to 8%, although higher rates, 12%, were detected in kidney transplant children, in a group of HIV-positive patients, and in healthy females. In this study, serum samples from pregnant women, together with those from non-pregnant women, were analyzed to check the prevalence of IgG Ab reacting to SV40 LT antigens. Serum samples were collected from pregnant and non-pregnant women, with the same mean age. Women were in the range of 15–48 years old. Samples were assayed by an indirect ELISA employing specific SV40 LT mimotopes as antigens, whereas functional analysis was performed by neutralization of the viral infectivity in cell cultures. As a control, sera were analyzed for Ab against BK polyomavirus (BKPyV), which is a human polyomavirus homologous to SV40. Statistical analyses employed chi-square with Yates’ correction, and Student’s t tests. Indirect ELISAs indicated that pregnant women tested SV40 LT-positive with a prevalence of 17% (23/134), whereas non-pregnant women had a prevalence of 20% (36/180) (P > 0.05). Ab against BKPyV were detected with a prevalence of 80% in pregnant women and with a prevalence of 78% in non-pregnant women. These data indicate that SV40 infects at a low prevalence pregnant women. We may speculate that SV40, or a close human polyomavirus still undetected, could be transmitted from mother to fetus.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Serum IgG Antibodies from Pregnant Women Reacting to Mimotopes of Simian Virus 40 Large T Antigen, the Viral Oncoprotein

et al. 2017

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“…These SV40-positive human sera, from our collection, were analyzed before by SV40 neutralization assay [19], [20]. The human peptide hNPS, a.a. sequence SFRNGVGTGMKKTSFQRAKS [34] was employed as a negative control peptide [20], [22].…”

Section: Methodsmentioning

confidence: 99%

“…SV40, a monkey neurotropic polyomavirus, is responsible for the progressive multifocal leukoencephalopathy (PML) in immune-compromised macaques [15], [16] while in humans its footprints have been detected in brain tumors and neurologic disorders [17], [18], [19].…”

Section: Introductionmentioning

confidence: 99%

Significant Low Prevalence of Antibodies Reacting with Simian Virus 40 Mimotopes in Serum Samples from Patients Affected by Inflammatory Neurologic Diseases, Including Multiple Sclerosis

et al. 2014

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Many investigations were carried out on the association between viruses and multiple sclerosis (MS). Indeed, early studies reported the detections of neurotropic virus footprints in the CNS of patients with MS. In this study, sera from patients affected by MS, other inflammatory (OIND) and non-inflammatory neurologic diseases (NIND) were analyzed for antibodies against the polyomavirus, Simian Virus 40 (SV40). An indirect enzyme-linked immunosorbent assay (ELISA), with two synthetic peptides, which mimic SV40 antigens, was employed to detect specific antibodies in sera from patients affected by MS, OIND, NIND and healthy subjects (HS). Immunologic data indicate that in sera from MS patients antibodies against SV40 mimotopes are detectable with a low prevalence, 6%, whereas in HS of the same mean age, 40 yrs, the prevalence was 22%. The difference is statistically significant (P = 0.001). Significant is also the difference between MS vs. NIND patients (6% vs. 17%; P = 0.0254), whereas no significant difference was detected between MS vs OIND (6% vs 10%; P>0.05). The prevalence of SV40 antibodies in MS patients is 70% lower than that revealed in HS.

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Significant association between human osteosarcoma and simian virus 40

Mazzoni

Benassi

Corallini

et al. 2014

Cancer

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BACKGROUND:Simian virus 40 (SV40) has been considered to be an oncogenic viral agent in the development of osteosarcoma (OS), which to the authors' knowledge continues to be of unknown etiology. METHODS: In the current study, serum samples from patients with OS were investigated with an indirect enzyme-linked immunoadsorbent assay (ELISA) to test for the presence of immunoglobulin G antibodies, which react with SV40 antigens. In ELISA, SV40 antigens were represented by 2 synthetic polypeptides that mimic epitopes of the viral capsid proteins 1 to 3. Additional sera from patients with breast cancer and undifferentiated nasopharyngeal carcinoma as well as healthy subjects were the controls. RESULTS: Immunologic results suggested that antibodies that react with SV40 mimotopes were more prevalent (44%) in serum samples from patients with OS compared with healthy subjects (17%). The difference in prevalence between these cohorts was statistically significant (P<.001). It is interesting to note that in the patients with OS, significance indicated the difference between OS versus breast cancer (44% vs 15%; P<.001) and OS versus undifferentiated nasopharyngeal carcinoma (44% vs 25%; P<.05). CONCLUSIONS: The data from the current study indicate an association between OS and SV40. These data could be transferred to clinical applications for innovative therapies to address SV40-positive OS. Cancer 2015;121:708-15. V C 2014 American Cancer Society.KEYWORDS: osteosarcoma, simian virus 40 (SV40), antibody, viral agent. INTRODUCTIONHuman osteosarcoma (OS) is a rare malignant neoplasm of the bone that for the most part affects children and young adolescents.1 To the best of our knowledge, little is known regarding the biology and pathology of this bone tumor. Cellular and animal experimental models were used in an attempt to elucidate the mechanism of OS onset.2-5 OS has multiple genetic risk factors, including groups of genes involved in cell proliferation/cell cycle/DNA damage repair. It has been reported that the incidence of OS is increased significantly in patients with specific hereditary diseases.

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Significant prevalence of antibodies reacting with simian virus 40 mimotopes in sera from patients affected by glioblastoma multiforme

Cited by 24 publications

References 38 publications

Serum IgG Antibodies from Pregnant Women Reacting to Mimotopes of Simian Virus 40 Large T Antigen, the Viral Oncoprotein

Serum IgG Antibodies from Pregnant Women Reacting to Mimotopes of Simian Virus 40 Large T Antigen, the Viral Oncoprotein

Significant Low Prevalence of Antibodies Reacting with Simian Virus 40 Mimotopes in Serum Samples from Patients Affected by Inflammatory Neurologic Diseases, Including Multiple Sclerosis

Significant association between human osteosarcoma and simian virus 40

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