Schiff Base Metal Derivatives Enhance the Expression of HSP70 and Suppress BAX Proteins in Prevention of Acute Gastric Lesion

Abstract

Schiff base complexes have appeared to be promising in the treatment of different diseases and disorders and have drawn a lot of attention to their biological activities. This study was conducted to evaluate the regulatory effect of Schiff base metal derivatives on the expression of heat shock proteins (HSP) 70 and BAX in protection against acute haemorrhagic gastric ulcer in rats. Rats were assigned to 6 groups of 6 rats: the normal control (Tween 20 5% v/v, 5 mL/kg), the positive control (Tween 20 5% v/v, 5 mL/kg), and four Schiff base derivative groups named Schiff_1, Schiff_2, Schiff_3, and Schiff_4 (25 mg/kg). After 1 h, all of the groups received ethanol 95% (5 mL/kg) but the normal control received Tween 20 (Tween 20 5% v/v, 5 mL/kg). The animals were euthanized after 60 min and the stomachs were dissected for histology (H&E), immunohistochemistry, and western blot analysis against HSP70 and BAX proteins. The results showed that the Schiff base metal derivatives enhanced the expression of HSP70 and suppressed the expression of BAX proteins during their gastroprotection against ethanol-induced gastric lesion in rats.

Gastroprotective Effects of Lion’s Mane MushroomHericium erinaceus(Bull.:Fr.) Pers. (Aphyllophoromycetideae) Extract against Ethanol-Induced Ulcer in Rats

“…In the gastric mucosa, it has been reported that HSP70 has important cytoprotective functions in vitro and in vivo [ 32 ]. In accordance with several studies [ 33 – 35 ], the present finding showed remarkable upregulation of the expression of HSP70 in rats pre-treated with mushroom extracts. The HSP70 family functions as a molecular chaperone and reduces stress-induced denaturation and aggregation of intracellular proteins.…”

Evidence of the gastroprotective and anti-Helicobacter pylori activities of β-mangostin isolated from Cratoxylum arborescens (vahl) blume

“…A previous study proved the essential role of HSP70 in the mucosal cytoprotection. 55 This effect may be prompted via the inhibition of the cell apoptotic process 56 through its chaperone function, other than that of the immune response. 57 An earlier study reported the critical involvement of HSP70 protein in the gastroprotection mechanisms against ethanol-induced gastric ulcer.…”

Apoptosis as a mechanism for burn-induced gastric mucosal injury

“…Bax/Bak forms oligomers on the outer mitochondrial surface and activates the caspase cascade (28). Studies have shown that programmed cell death and increased Bax protein expression play an important role gastric injuries induced by NSAID (29), ethanol (30,31) and water immersion-restraint stress (32).…”