Bioremediation of pesticide contaminated water using an organophosphate degrading enzyme immobilized on nonwoven polyester textiles

Quick and low cost immobilization of proteinases on polyesters: Comparison of lactobacilli cell-envelope proteinase and trypsin for protein degradation

“…Immobilization of enzymes on polyester was based on the method previously described (Gao et al, 2014), with further optimization noted below. Polyester fabric discs (1.0 cm diameter, ∼2.5 mg each) were treated with EDA (3 discs per 1 mL) on a shaker followed by thorough rinsing with Milli-Q water.…”

An efficient thermostable organophosphate hydrolase and its application in pesticide decontamination

“…Among enzymes that catalyze the hydrolysis of phosphoester or phosphothiol bonds in OPs, the best characterized are PTE from Brevundimonas diminuta (Carletti et al, ), OpdA from Agrobacterium radiobacter (Ely et al, ) and the mammalian paraoxonase (PON) (Valiyaveettil et al, ). Some of them, such as OpdA, are commercially available and have been employed to remediate OP‐contaminated water (Gao et al, ), but the main problem associated with the use of these enzymes lies in their low stability in solution, which makes the costs for their application problematically high.…”

Improving storage stability of recombinant organophosphorus hydrolase

“…OPH hydrolyzes and inactivates variety of OP-compounds with high efficiency [11,13]. Thus, OPH is a strong candidate for the development of an agent not only for the therapeutic intervention of OP-poisoning in humans and other animals but also for the development of an environmental-friendly mean for decontamination (clean-up) of OPcontaminated objects and areas [4,11,[14][15][16][17]. However, purified OPH enzyme loses its activity on storage [18][19][20] and for successful commercial applications and use, long-term storage stability of purified OPH enzyme is important.…”