Three Outbreak-causing<i>Neisseria meningitidis Serogroup</i>C Clones, Brazil1

Barroso, David E.; Castiñeiras, Terezinha Marta Pereira Pinto; Freitas, Fernanda; Marsh, Jane W.; Krauland, Mary G.; Tulenko, Mary M.; Fonseca, Érica L.; Vicente, Ana Carolina Paulo; Rebelo, Maria Cristina; Cerqueira, Elaine O.; Xavier, Adriano C.; Cardozo, Ana P.C.M.; Silva, Simone E.M. da; Harrison, Lee H.

doi:10.3201/13-0610

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…This change was associated with a marked decline in the major serogroup B sub-lineages within cc32 (ST-5996, ST-639 and ST-33) and ongoing persistence of serogroup C strains belonging cc103 (Figure S1A-B). Similarly, serogroup C re-emerged as the major cause of meningococcal disease during the 2000s in other parts of Brazil, where it has been associated with outbreaks and, as in the present study, a high case-fatality rate [4]. The data from this study will serve as a baseline by which to measure the impact in Amazonas of the 2010 introduction of serogroup C conjugate vaccine into the infant immunization program.…”

Section: Discussionmentioning

confidence: 62%

“…Serogroup C Neisseria meningitidis replaced serogroup B as the predominant serogroup during the 2000s in Brazil, which led to introduction of serogroup C conjugate vaccine into the paediatric immunization schedule in 2010 [3]. This serogroup replacement was associated with outbreaks and high case fatality rates, with outbreak-causing strains belonging genetically to different clonal complexes (cc): cc11, cc32, and cc103 [4].…”

Section: Introductionmentioning

confidence: 99%

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Neisseria meningitidis disease-associated clones in Amazonas State, Brazil

Silva

Coronato

Schlackman

et al. 2018

Infectious Diseases

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We demonstrate a high rate of meningococcal disease in Amazonas State, where, like other parts of Brazil, serogroup C replaced serogroup B during 2000s. These data serve as a baseline to measure impact of serogroup C conjugate vaccine introduction in 2010. This study emphasizes the need for enhanced surveillance to monitor changes in meningococcal disease trends following the introduction of meningococcal vaccines.

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Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Neisseria meningitidis disease-associated clones in Amazonas State, Brazil

Silva

Coronato

Schlackman

et al. 2018

Infectious Diseases

Self Cite

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“…The majority of the meningococcal outbreak isolates belonged to hyper-invasive lineages CC32, CC41/44, CC269, and CC11 38 – 40 . With the addition of FetA and PorA types to the 7 gene MLST scheme, US outbreak strain genotypes matched isolates associated with outbreaks reported in Italy and Brazil 39 , 41 , 42 . Some were seen among a small proportion, 0.6–8.5%, of US sporadic isolates collected through Active Bacterial Core Surveillance (2000–2014).…”

Section: Discussionmentioning

confidence: 85%

Whole genome sequencing for investigations of meningococcal outbreaks in the United States: a retrospective analysis

Whaley

Joseph

Retchless

et al. 2018

Sci Rep

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Although rare in the U.S., outbreaks due to Neisseria meningitidis do occur. Rapid, early outbreak detection is important for timely public health response. In this study, we characterized U.S. meningococcal isolates (N = 201) from 15 epidemiologically defined outbreaks (2009–2015) along with temporally and geographically matched sporadic isolates using multilocus sequence typing, pulsed-field gel electrophoresis (PFGE), and six whole genome sequencing (WGS) based methods. Recombination-corrected maximum likelihood (ML) and Bayesian phylogenies were reconstructed to identify genetically related outbreak isolates. All WGS analysis methods showed high degree of agreement and distinguished isolates with similar or indistinguishable PFGE patterns, or the same strain genotype. Ten outbreaks were caused by a single strain; 5 were due to multiple strains. Five sporadic isolates were phylogenetically related to 2 outbreaks. Analysis of 9 outbreaks using timed phylogenies identified the possible origin and estimated the approximate time that the most recent common ancestor emerged for outbreaks analyzed. U.S. meningococcal outbreaks were caused by single- or multiple-strain introduction, with organizational outbreaks mainly caused by a clonal strain and community outbreaks by divergent strains. WGS can infer linkage of meningococcal cases when epidemiological links are uncertain. Accurate identification of outbreak-associated cases requires both WGS typing and epidemiological data.

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Investigating the immunodominance of carbohydrate antigens in a bivalent unimolecular glycoconjugate vaccine against serogroup A and C meningococcal disease

et al. 2014

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Multicomponent constructs, obtained by coupling different glycans to the carrier protein, have been proposed as a way to co-deliver multiple surface carbohydrates targeting different strains of one pathogen and reduce the number of biomolecules in the formulation of multivalent vaccines. To assess the feasibility of this approach for anti-microbial vaccines and investigate the potential immunodominance of one carbohydrate antigen over the others in these constructs, we designed a bivalent unimolecular vaccine against serogroup A (MenA) and C (MenC) meningococci, with the two different oligomers conjugated to same molecule of carrier protein (CRM197). The immune response elicited in mice by the bivalent MenAC construct was compared with the ones induced by the monovalent MenA and MenC vaccines and their combinations. After the second dose, the bivalent construct induced good levels of anti-MenA and anti-MenC antibodies with respect to the controls. However, the murine sera from the MenAC construct exhibited good anti-MenC bactericidal activity, and very low anti-MenA functionality when compared to the monovalent controls. This result was explained with the diverse relative avidities against MenA and MenC polysaccharides, which were measured in the generated sera. The immunodominant effect of the MenC antigen was fully overcome following the third immunization, when sera endowed with higher avidity and excellent bactericidal activity against both MenA and MenC expressing strains were elicited. Construction of multicomponent glycoconjugate vaccines against microbial pathogens is a feasible approach, but particular attention should be devoted to study and overcome possible occurrence of immune interference among the carbohydrates.

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Three Outbreak-causingNeisseria meningitidis SerogroupC Clones, Brazil1

Cited by 5 publications

References 10 publications

Neisseria meningitidis disease-associated clones in Amazonas State, Brazil

Neisseria meningitidis disease-associated clones in Amazonas State, Brazil

Whole genome sequencing for investigations of meningococcal outbreaks in the United States: a retrospective analysis

Investigating the immunodominance of carbohydrate antigens in a bivalent unimolecular glycoconjugate vaccine against serogroup A and C meningococcal disease

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