Bacteriophage types of methicillin-resistant Staphylococcus aureus in a tertiary care hospital

Kali, Arunava; Spellman, Stephen R.; Umadevi, S.; Kumar, Shailesh; Joseph, Noyal Mariya; Srirangaraj, Sreenivasan; Easow, Joshy M

doi:10.4066/amj.2013.1742

Cited by 6 publications

(4 citation statements)

References 28 publications

(21 reference statements)

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“…). Regarding the phage -groups, no predominance of a particular phage-group was observed, though as expected all the S.aureus isolated here fall among phage-group I,II and III(table VIII),therefore the data of the typing did not show a particular strain(s) of S.aureus of importance, since there is a known phage-type (47/53/75/77) which is usually encountered in hospital environment [42,43]. The absence of this phage-type and predominating one in the present work could possibly by due either to strain differentiation or to the small number of staphylococcal isolates (76 isolates), therefore the phage-typing of S.aureus particularly in the hospital environment, need further investigation and elaborate study.…”

Section: Staphylococcus Aureus Phage-typingsupporting

confidence: 66%

Isolation and Identification of Bacterial Wound Infection Isolates and their Antibiotic Susceptibility Pattern

M. R. Alshara

2018

Kufa Jour. Bio.

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Wound Infections are continuous to be a major complication with significant increase in costs, morbidity and potential mortality. This study was aim to screening the bacteriological observation on wound infection in both inpatients and outpatients, the effective antibiotic and serotyping for the prevalent bacteria. The study was carried out on two hundred patients of different age groups to isolate the causative organisms encountered in wound infection and their antibiotics. The percentage of wound infection among outpatients was found to be 84%, similarly the percentage of wound contamination inside the hospital (Medical City Teaching Hospital) also was found to be 84%. The relation between sex and wound infection was studied, no effect of sex on the rate of infection was found.A total of 152 bacterial isolates were predominant Staphylococcus aureus (76, 50%), followed by Escherichia coli (36, 23.69%), Beta-haemolytic streptococci(13,8.55%),Klebsiella aerogenes (8,5.26%), Pseudomonas aeruginosa(7,4.6%), Proteus spp.

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Section: Staphylococcus Aureus Phage-typingsupporting

confidence: 66%

Isolation and Identification of Bacterial Wound Infection Isolates and their Antibiotic Susceptibility Pattern

M. R. Alshara

2018

Kufa Jour. Bio.

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“…Also in diagnostics, the use of phages as a tool for S. aureus typing because of their high specificity has a long history (27,28). Although it is still in use in some countries (29), this method has been widely replaced by new molecular methods. Recently, novel bacteriophage-based diagnostic applications have appeared, as reviewed elsewhere (22,30).…”

Section: Discussionmentioning

confidence: 99%

Bacteriophage-Based Latex Agglutination Test for Rapid Identification of Staphylococcus aureus

Idelevich

Walther²,

Molinaro³

et al. 2014

J Clin Microbiol

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e Rapid diagnosis is essential for the management of Staphylococcus aureus infections. A host recognition protein from S. aureus bacteriophage phiSLT was recombinantly produced and used to coat streptavidin latex beads to develop a latex agglutination test (LAT). The diagnostic accuracy of this bacteriophage-based test was compared with that of a conventional LAT, Pastorex StaphPlus, by investigating a clinical collection of 86 S. aureus isolates and 128 coagulase-negative staphylococci (CoNS) from deep tissue infections. All of the clinical S. aureus isolates were correctly identified by the bacteriophage-based test. While most of the CoNS were correctly identified as non-S. aureus isolates, 7.9% of the CoNS caused agglutination. Thus, the sensitivity of the bacteriophage-based LAT for identification of S. aureus among clinical isolates was 100%, its specificity was 92.1%, its positive predictive value (PPV) was 89.6%, and its negative predictive value (NPV) was 100%. The sensitivity, specificity, PPV, and NPV of the Pastorex LAT for the identification of S. aureus were 100%, 99.2%, 98.9%, and 100%, respectively. Among the additionally tested 35 S. aureus and 91 non-S. aureus staphylococcal reference and type strains, 1 isolate was false negative by each system; 13 and 8 isolates were false positive by the bacteriophage-based and Pastorex LATs, respectively. The ability of the phiSLT protein to detect S. aureus was dependent on the presence of wall teichoic acid (WTA) and corresponded to the production of ribitol phosphate WTA, which is found in most S. aureus clones but only a small minority of CoNS. Bacteriophage-based LAT identification is a promising strategy for rapid pathogen identification. Finding more specific bacteriophage proteins would increase the specificity of this novel diagnostic approach.

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“…Until the early 1990s and the introduction of the first method of molecular typing of microorganisms -PFGE (Pulsed-Field Gel Electrophoresis), phagotyping was the main method used in typing of clinical S. aureus strains [17]. Currently, this method is less frequently used, but it can provide valuable information about the prevalence of epidemic S. aureus strains [21].…”

Section: Phage Typingmentioning

confidence: 99%

Overview of phenotypic methods used for differentiation ofStaphylococcus aureus

Kasela

Malm

2018

Current Issues in Pharmacy and Medical Sciences

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Choosing the appropriate method for differentiation of Staphylococcus aureus strains is important for effective diagnostics and epidemiological investigations. Despite the fact that the results of phenotypic methods are strongly dependent on environmental conditions, they can still be useful in the investigation of epidemic strains of S. aureus. In this article, the potential application of commonly used phenotypic methods in epidemiological studies of S. aureus was analysed. Advantages and disadvantages of methods such as biotyping, serotyping, phage typing, AST (Antimicrobial Susceptibility Testing), SDS-PAGE (Sodium Dodecyl Sulphate Polyacryl Gel Electrophoresis), MLEE (Multilocus Enzyme Electrophoresis) and MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectroscopy) were also discussed. Finally, phenotypic techniques were compared in terms of their discriminatory potential, typeability of isolates, time of analysis, reproducibility, ease of performance and ease of results interpretation.

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Bacteriophage types of methicillin-resistant Staphylococcus aureus in a tertiary care hospital

Abstract: We found MRSA strain diversity in hospital wards with differences in their antibiotic susceptibility pattern. The findings may impact infection control and antibiotic policy significantly.

Cited by 6 publications

References 28 publications

Isolation and Identification of Bacterial Wound Infection Isolates and their Antibiotic Susceptibility Pattern

Isolation and Identification of Bacterial Wound Infection Isolates and their Antibiotic Susceptibility Pattern

Bacteriophage-Based Latex Agglutination Test for Rapid Identification of Staphylococcus aureus

Overview of phenotypic methods used for differentiation ofStaphylococcus aureus

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