Overview of phenotypic methods used for differentiation of<i>Staphylococcus aureus</i>

Kasela, Martyna; Malm, Anna

doi:10.1515/cipms-2018-0023

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Produces a biofilm protecting itself from the antibiotics administered topically (Mekić, Matanović and Šeol, 2011). (Speers, Olma and Gilbert, 1998;Kasela and Malm, 2018;Schissler et al, 2009) (Schissler et al, 2009). As a result, these tests cannot accurately distinguish between S. aureus and S. pseudintermedius veterinary strains (Couto et al, 2001).…”

Section: Other Bacterial Colonisation Of Dogsmentioning

confidence: 99%

Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa

Prior

Moodley

Karama

et al. 2022

Journal of the South African Veterinary Association

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© © U Un ni iv ve er rs si it ty y o of f P Pr re et to or ri ia a i _________________________________________________________________________________________________________ Dedication _________________________________________________________________________________________________________ This dissertation is dedicated to those who have supported me during this thesis. My parents, Lisa and Martin Prior, who have been the pillars of support throughout my long years of study. I would not be standing where I am today without your direction and encouragement to fulfil my dreams, "you lose everything when you don't love yourself". My partner, Dr Brittany Fourie, whose unwavering love and kind-heartedness throughout the writing process kept me motivated, "your beauty is undeniable but everything sacred and ancient in you is even more stunning". My friend, colleague, supervisor, Andrew, whose unfaltering patience, wisdom and benevolence throughout the writing process ensured that I always produced nothing but excellence, "your life is captured in a story, the weak trumps the evil villain, your own story should resonate and fill you within. Your story should be providential, not Darwinian". Most importantly and above all else, I thank my heavenly Father for His love and guidance. "For the Spirit God gave us does not make us timid, but gives us power, love, and self-discipline" -Timothy 1:7. This dissertation is in honour of all of you. © © U Un ni iv ve er rs si it ty y o of f P Pr re et to or ri ia a ii _________________________________________________________________________________________________________ Declaration of Originality/Plagiarism _________________________________________________________________________________________________________ By submitting this dissertation for the degree MSc (Veterinary Science) at the University of Pretoria, I declare that the entirety of the work contained therein is my own original work, that I am the sole author thereof (save to the extent explicitly otherwise stated), that reproduction and publication thereof by Pretoria University will not infringe any third party rights, and that I have not previously in its entirety or in part submitted it for obtaining any qualification at this or any other tertiary institution. Declaration of originality attached as appendix (chapter 8). © © U Un ni iv ve er rs si it ty y o of f P Pr re et to or ri ia a iii

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Section: Other Bacterial Colonisation Of Dogsmentioning

confidence: 99%

Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa

Prior

Moodley

Karama

et al. 2022

Journal of the South African Veterinary Association

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“…It is also reported to be able to discriminate MRSA from MSSA ( Alharbi et al, 2021 ). However, nowadays, MAL-DI-TOF MS is not commonly used in epidemiological studies due to the lack of guidelines for its validity, sensitivity and performance ( Kasela and Malm, 2018 , Shell et al, 2017 ). Furthermore, it is often time-consuming and laborious.…”

Section: Typing Methods For Bovine Staphylococcus Aureusmentioning

confidence: 99%

Usefulness of molecular typing methods for epidemiological and evolutionary studies of Staphylococcus aureus isolated from bovine intramammary infections

Chadi¹,

Dib²,

Zeroual³

et al. 2022

Saudi Journal of Biological Sciences

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Genetic relationship of Staphylococcus aureus isolated from humans, animals, environment, and Dangke products in dairy farms of South Sulawesi Province, Indonesia

et al. 2022

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Background and Aim: Staphylococcus aureus is a bacterium that causes several infectious diseases, including mastitis, endocarditis, and osteomyelitis, and poses a threat to human and animal health. This study aims to phenotypically and genetically identify S. aureus from the isolates collected from humans, animals, environment, and Dangke products in the dairy farms of South Sulawesi Province, Indonesia, as well as to establish a genetic relationship among the isolated S. aureus strains. Materials and Methods: The total number of samples was 142, comprising 30 humans (skin swab), 58 animals (raw milk), 14 dairy products (Dangke), and 40 environmental samples (water). S. aureus was phenotypically identified using the culture method, followed by Gram staining, catalase test, and coagulase test. Simultaneously, genotypic identification of S. aureus was performed using the conventional polymerase chain reaction and sequencing methods. Sequencing data were analyzed using the MEGA X software by comparing BLAST National Center for Biotechnology Information databases. Results: The phenotypic methods revealed that 56/142 (39.4%) animal, human, and Dangke samples grew on culture, and 56/56 (100%) were Gram stain positive, 56/56 (100%) catalase-positive, and 23/56 (41.1%) coagulase positive. The genotypic method revealed that 32/56 (57.1%) samples amplified the nuc gene. The phylogenetic analysis of 12 isolates revealed that they are all closely related and do not belong to distinct clades. Conclusion: It indicates that S. aureus isolates from animals (S30) are probably the same strain as human isolates (H2, H3, H4, and H5). The findings of this study can be used as information regarding the importance of preventing and controlling diseases caused by S. aureus using a health approach involving the human, animal, and environmental sectors. This study was limited to the sequencing analysis of the nuc gene.

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Overview of phenotypic methods used for differentiation ofStaphylococcus aureus

Cited by 3 publications

References 24 publications

Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa

Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa

Usefulness of molecular typing methods for epidemiological and evolutionary studies of Staphylococcus aureus isolated from bovine intramammary infections

Genetic relationship of Staphylococcus aureus isolated from humans, animals, environment, and Dangke products in dairy farms of South Sulawesi Province, Indonesia

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