Photothermal ablation of tumor cells using a single-walled carbon nanotube–peptide composite

Abstract

Single-walled carbon nanotubes (SWCNTs) are known to have great potential for biomedical applications such as photothermal ablation of tumor cells in combination with near-infrared (NIR) irradiation. In this study, the photothermal activity of a novel SWCNTs composite with a designed peptide having a repeated structure of H-(-Lys-Phe-Lys-Ala-)7-OH [(KFKA)7] against tumor cells was evaluated in vitro and in vivo. The SWCNT-(KFKA)7 composite demonstrated high aqueous dispersibility that enabled SWCNTs to be used in tumor ablation. The NIR irradiation of SWCNT-(KFKA)7 solution resulted in a rapid temperature increase dependent on the SWCNTs concentration up to 50μg/ml. Three minutes of NIR irradiation of a colon 26 or HepG2 cell culture incubated with SWCNT-(KFKA)7 resulted in remarkable cell damage, while that by single treatment with SWCNT-(KFKA)7 or NIR irradiation alone was moderate. The intratumoral injection of SWCNT-(KFKA)7 solution followed by NIR irradiation resulted in a rapid increase of the temperature to 43°C in the subcutaneously inoculated colon 26 tumor based on thermographic observation and remarkable suppression of tumor growth compared with treatment with only SWCNT-(KFKA)7 injection alone or NIR irradiation alone. These results suggest the a great potential of an SWCNT-peptide composite for use in photothermal cancer therapy.

Single-Walled Carbon Nanohorn (SWNH) Aggregates Inhibited Proliferation of Human Liver Cell Lines and Promoted Apoptosis, Especially for Hepatoma Cell Lines

“…This suggests the great potential of an SWCNTpeptide composite for use in photothermal cancer therapy. 46 We obtained similar results using L02 and HepG2 cells treated with SWNHs at lower dosages (1.5-6.0 µg/mL, 4 mL of culture matrix).…”

“…Single-walled carbon nanotubes (SWCNTs) are known to have great potential for biomedical applications such as photothermal ablation of tumor cells in combination with near-infrared irradiation. Hashida et al 46 found that the photothermal activity of a novel SWCNT composite with a designed peptide having a repeated structure of H -( -Lys-Phe-Lys-Ala-)7-OH ([KFKA]7) against tumor cells. The SWCNTs-(KFKA)7 composite demonstrated high aqueous dispersibility that enabled SWCNTs to be used in tumor ablation.…”

Photothermal therapy of melanoma tumor using multiwalled carbon nanotubes

“…39 In this study, we used these NPs to evaluate their ability for tumor size reduction by PTT technique. At first, for improving the dispersibility of MWNTs in water, the hydrophilic polymer PEG was used to wrap the surface of the nanotubes.…”

“…Hyperthermia also increases the vascular permeability of tumor compared with normal vasculature, which can facilitate the delivery of anticancer drugs into tumors. 39 Therefore, localization of plasmonic NPs in the tumor region can be an efficient strategy for treatment of solid tumors. In this in vivo ablation experiment, O-CNT-PEG was injected into the melanoma tumor, and then the solid tumor region was irradiated.…”

Calentamiento por Fotoactivación de NanoTubos de Carbono de pared simple Funcionalizados con Ácido Fólico (NTC-AF)

“…En consecuencia los efectos térmicos generados por NTC pueden tener ventajas terapéuticas importantes, ya que estudios fármaco-cinéticos han observado que los NTC internalizados en ratones por diferentes procedimientos no generan efectos tóxicos ( Hashida, 2014). El uso de la radiación IR (700 -1100-nm) para la inducción de la hipertermia es particularmente atractiva porque los tejidos vivos no absorben fuertemente en este rango y la luz NIR externa puede penetrar el tejido normal sin problemas y permitir la ablación de las células en las cuales se hallan los NTC.…”