Assessment of chondrogenic differentiation potential of autologous activated peripheral blood stem cells on human early osteoarthritic cancellous tibial bone scaffold

Abstract

AAPBSC initiated chondrocyte differentiation on an autologous cancellous bone scaffold, and the addition of PRP and hG-CSF further stimulated cell proliferation toward a chondrocyte phenotype with potentiated Sox9 transcription resulting in sequential COL-2 and aggrecan mRNA increases that ultimately resulted in histologically confirmed increased proteoglycan and glucosaminoglycan content in newly formed hyaline cartilage.

Avoidance of total knee arthroplasty in early osteoarthritis of the knee with intra-articular implantation of autologous activated peripheral blood stem cells versus hyaluronic acid: A randomized controlled trial with differential effects of growth factor addition

“…chondrocyte precursors, paracrine support, or a combination of all of the above [17,22]. In the present study, we performed a RCT using two AAPBSC groups (one with and one without IA hG-CSF; double blinded between the groups) and compared their outcome with that of a nonstem cell group that only received HA (open between the AAPBSC groups and the nonstem cell control group).…”

“…In a pilot study, Turajane et al [18] demonstrated that a similar combination of IA AAPBSC with or without GFA along with HA in conjunction with arthroscopic MCS resulted in quality-of-life improvements measured by WOMAC and KOO scores and succeeded in regenerating articular cartilage in early osteoarthritic knee disease that failed conservative treatment versus noncellular interventions without adverse reactions. Subsequently, in their assessment of the chondrogenic differentiation potential of AAPBSC on human early osteoarthritic cancellous tibial bone scaffolds seeded with hG-CSF-mobilized PB-derived stem cells for cartilage regeneration, temporally and sequentially increased expression of cartilage-relevant genes such as Sox9, collagen type II (COL-2), and aggrecan (AGGR) as well as histological evidence of increased and incorporated proteoglycan and glycosaminoglycan contents was demonstrated [17]. A later RCT, also by the group of Saw et al [14], similarly documented (via MRI and histological evaluations) positive clinical outcomes at 24 months using autologous PBSC, versus HA controls.…”

“…Cellular therapy for human adult use usually means that autologous nucleated cells are harvested via either bone marrow (BM) aspiration or peripheral blood (PB) stem cell collection through leukapheresis or via liposuction [11]. Cellular therapies for treating various stages of human OA have recently provided successful and encouraging clinical and laboratory results using the combination of intra-articular (IA) autologous activated peripheral blood stem cells (AAPBSCs) [12][13][14][15][16] with or without growth factor addition (GFA) [17,18] along with hyaluronic acid (HA) in conjunction with arthroscopic microdrilling mesenchymal cell stimulation (MCS) in regenerating articular cartilage. In this randomized controlled trial, in early OA that failed conservative intervention, the clinical outcomes [primary endpoint: the need for total knee arthroplasty (TKA); secondary endpoint: WOMAC scores] following a combination of arthroscopic microdrilling mesenchymal cell stimulation (MCS) and repeated intraarticular (IA) autologous activated peripheral blood stem cells (AAPBSCs) with and without growth factor addition (GFA) along with hyaluronic acid (HA) versus IA-HA alone were evaluated.…”

Association between leukocyte telomere length and angiogenic cytokines in knee osteoarthritis

“…Interestingly, G-CSF can stimulate peripheral blood stem cell proliferation and differentiation toward chondrocyte phenotype on an autologous cancellous bone scaffold. 17 Recently, it has been shown that G-CSF-mobilized peripheral blood stem cells facilitate articular regeneration in OA. 18 Hepatocyte growth factor, a heparin-binding glycoprotein from the kringle-serine proteinase superfamily, was originally cloned and identified as a mitogen for mature hepatocytes.…”

Hydroxychloroquine (HCQ) induces inhibition of Col-II and COMP expression in the chondrocytes

“…The collagen type II functions to facilitate the adherence of cells together along with the maintenance of cell and extracellular matrix interactions (Turajane et al, 2014). The process of cell signal transfer, protein activation and expression of genes depend on the interaction of collagen type II binding and its ligand (Guzman-Morales et al, 2014).…”