Using a Caesalpinia echinata Lam. protease inhibitor as a tool for studying the roles of neutrophil elastase, cathepsin G and proteinase 3 in pulmonary edema

Cruz-Silva, Ilana; Neuhof, Christiane; Gozzo, Andrezza Justino; Nunes, Viviane Abreu; Hirata, Izaura Yoshico; Sampaio, Misako U.; Figueiredo-Ribeiro, Rita de Cássia L.; Neuhof, H.; Araújo, Mariana da Silva

doi:10.1016/j.phytochem.2013.09.025

Cited by 9 publications

(15 citation statements)

References 47 publications

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“…Because of the versatility in inhibiting different enzymes, bifunctional inhibitors have been investigated to understand their mechanistic inhibitory strategies, in order to elucidate their interaction with their target enzymes (Migliolo et al 2010) and their potential applications as therapeutic drugs to treat cancer and to control pests and crop diseases in transgenic plants (Pandey et al 2007, Mosolov & Valueva 2008, Paula et al 2012, Cruz-Silva et al 2013.…”

Section: Discussionmentioning

confidence: 99%

Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions

Brito

Melo

Alves

et al. 2016

Hoehnea

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-(Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions). The crude extract and protein fractions of Hymenaea courbaril L. seeds were investigated for the presence of trypsin and papain inhibitors and antimicrobial activity against Vibrio parahaemolyticus, Staphylococcus aureus, and Escherichia coli. Protein fractions were obtained from the crude extract after precipitation with ammonium sulfate into three saturation ranges (0-30%, 30-60%, and 60-90%), called Hc030, Hc3060, and Hc6090, respectively. The crude extract and protein fractions inhibited trypsin and papain activity, but to different degrees. Antimicrobial activity was observed in Hc030 and Hc3060 fractions, but only against V. parahaemolyticus.The inhibitor isolated from the Hc3060 fraction was more effective in inhibiting trypsin (100% inhibition) than papain (54% inhibition), and showed an apparent molecular mass of 20 kDa. This study shows that H. courbaril seeds contain proteins with protease-inhibiting and antibacterial activity, indicating that this species is a source of bioactive compounds. Keywords: antimicrobial, cystatin, Fabaceae, protease RESUMO -(Purificação parcial de um inibidor de tripsina/papaína de sementes de Hymenaea courbaril L. e atividade antibacteriana de suas frações proteicas). O extrato bruto e frações proteicas de sementes de Hymenaea courbaril foram investigados para a presença de inibidores de tripsina e papaína e atividade antimicrobiana contra Vibrio parahaemolyticus, Staphylococcus aureus e Escherichia coli. As frações foram obtidas após precipitação do extrato bruto com sulfato de amônio em três faixas de saturação (0-30%, 30-60% e 60-90%) e denominadas Hc030, Hc3060 e Hc6090, respectivamente. O extrato bruto e as frações apresentaram diferente especificidade em inibir a atividade da tripsina e papaína. A atividade antimicrobiana foi observada nas frações Hc030 e Hc3060 e somente contra V. parahaemolyticus. O inibidor isolado a partir de Hc3060 foi mais eficiente na inibição da tripsina (100% de inibição) do que da papaína (54% de inibição), e mostrou uma massa molecular aparente de 20 kDa. Este estudo mostra que as sementes de H. courbaril possuem inibidores de proteases e proteínas com atividade antibacteriana, indicando que a espécie é uma fonte de compostos bioativos.

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Section: Discussionmentioning

confidence: 99%

Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions

Brito

Melo

Alves

et al. 2016

Hoehnea

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“…9,12,17 Most of these compounds had a potent and dual inhibitory effect on HNE and Pr3. Of these synthetics, a new 2-aminobenzaldehyde oxime analog, compound 6, exhibited the most potent inhibitory effects on the activity of HNE and Pr3 with IC 50 values of 0.05 and 0.22 lM, respectively, and was slightly potent than sivelestat (Tables 1 and 2).…”

Section: Effects Of Synthetics On Neutrophil Serine Proteasesmentioning

confidence: 99%

“…Thus, neutrophil proteases are considered a target in the treatment of ALI. 9,20,25 Herein, the protective effects of compound 6 in lung injury following LPS treatment were examined. The results showed no significant differences in MPO activity and histological analysis between the compound 6-treated group and the sham-treated animals.…”

Section: Compound 6 Diminished Lps-induced Paw Edema In Micementioning

confidence: 99%

“…6 However, recent reports suggest that excess proteolysis which results from an influx of neutrophils into the airways is pivotal in the progressive destruction of lung parenchyma, for example, acute lung injury (ALI), acute respiratory distress syndrome (ARDS), and chronic obstructive pulmonary disease (COPD). [7][8][9][10] Therefore, HNE and Pr3 may prove to be targets for the generation of agents in the treatment of neutrophilic inflammatory disease. 6,11 In 2000, an acetylating inhibitor, sivelestat, was developed for the treatment of ALI in Japan, and is the only nonpeptidic inhibitor http://dx.doi.org/10.1016/j.bmc.2014.12.056 0968-0896/Ó 2014 Elsevier Ltd. All rights reserved.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and pharmacological characterization of 2-aminobenzaldehyde oxime analogs as dual inhibitors of neutrophil elastase and proteinase 3

Hwang¹,

Wang²,

Wang³

et al. 2015

Bioorganic & Medicinal Chemistry

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“…It is known that the conservation of Caesalpinia echinata Lam., Brazilwood, for example, requires sub-zero temperatures (Hellmann et al, 2006), but the factors that lead to loss the viability of these seeds in less than 60 days when stored at or close to the ambient temperature are not known (Barbedo et al, 2002). This is a species that, in addition to its historical importance to Brazil and being one of the most important flora currently at risk of extinction and of its economic and pharmacological potential (Pilatti et al, 2011;Lamarca and Barbedo, 2012;Cruz-Silva et al, 2013;Gomes et al, 2014), produces seeds of great scientific interest because they are tolerant to desiccation, but with low storage capacity.…”

Section: Introductionmentioning

confidence: 99%

Viability of Brazilwood seeds (Caesalpinia echinata Lam.) stored at room temperature in controlled atmospheres

Neto

Barbedo

2015

J. Seed Sci.

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-Seed storage at room temperature is an important and low-cost tool for ex situ conservation. However, the high rates of seed deterioration could reduce the potential for storage in this condition. Therefore, the knowledge of the suitable water content for this type of storage plays a critical role. This study aimed to assess the time required to stabilize the relative humidity (RH) in sealed flasks with saturated salt solutions, with or without the introduction of seeds of Caesalpinia echinata, as well as to assess the viability of these seeds stored in environments with different hygroscopic equilibrium. The results showed that 2 and about 12 days are needed to stabilize the RH, respectively, without or with the seeds. The amount of saturated salt solutions in this airtight environment influences both the speed to equilibrate the RH and the final values of the RH. Seeds of Caesalpinia echinata tolerate drying up to 5% water content (wet basis); however, the viability of these seeds at room temperature is maintained for short periods even at low water content.Index terms: storage, hygroscopic equilibrium, desiccation tolerance.Viabilidade de sementes de pau-brasil (Caesalpinia echinata Lam.) mantidas em temperatura ambiente com atmosferas controladas RESUMO -O armazenamento de sementes em temperatura ambiente é uma importante ferramenta como estratégia de conservação ex situ. No entanto, as altas taxas de deterioração diminuem o potencial de armazenamento. Diante disso, a busca do teor de água ótimo para este tipo de armazenamento assume papel fundamental. Este trabalho teve por objetivo avaliar o período necessário para equilíbrio da umidade relativa do ar (UR) em frascos herméticos a partir da introdução de soluções salinas saturadas e de sementes de Caesalpinia echinata, bem como avaliar a capacidade de manutenção da viabilidade dessas sementes em ambientes com diferentes equilíbrios higroscópicos. Os resultados permitiram verificar que são necessários dois dias para equilíbrio da UR a partir da inclusão de soluções salinas saturadas em ambiente hermético sem a presença de sementes, e cerca de 12 dias com a presença das sementes; que a quantidade de soluções salinas presente no ambiente hermético influencia a velocidade de equilíbrio da UR e o equilíbrio ao final do período e as sementes de Caesalpinia echinata suportam secagem até teores de água de 5% (base úmida), mas mesmo com baixo teor de água estas sementes conservam-se por curtos períodos quando em temperatura ambiente.Termos para indexação: armazenamento, equilíbrio higroscópico, tolerância à dessecação.

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Using a Caesalpinia echinata Lam. protease inhibitor as a tool for studying the roles of neutrophil elastase, cathepsin G and proteinase 3 in pulmonary edema

Cited by 9 publications

References 47 publications

Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions

Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions

Synthesis and pharmacological characterization of 2-aminobenzaldehyde oxime analogs as dual inhibitors of neutrophil elastase and proteinase 3

Viability of Brazilwood seeds (Caesalpinia echinata Lam.) stored at room temperature in controlled atmospheres

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