Proteomic analysis of cervical vaginal fluid proteins among women in recurrent preterm labor

Lo, Jamie O.; Reddy, Ashok P.; Wilmarth, Phillip A.; Roberts, Victoria H. J.; Kinhnarath, Amanda; Snyder, Janice; Rincón, Mónica; Gravett, Michael G.; Nagalla, Srinivasa R.; Pereira, Leonardo

doi:10.3109/14767058.2013.852172

Cited by 14 publications

(14 citation statements)

References 25 publications

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“…This study revealed specific pro-inflammatory proteomic profiles Various proteins have been linked to intra-amniotic infection in non-human primates or recurrent spontaneous preterm birth in humans, [50][51][52] including several proteins identified in this study.…”

Section: Commentmentioning

confidence: 60%

“…Other previously documented biomarkers of preterm birth were also found to be differentially expressed in cases of CI including increased cysteine-rich secretory protein 3 levels, a protein released upon neutrophil degranulation, 51 and decreased levels of transforming growth factor beta receptor type 3 (TGFBR3), a protein with many functions including a role in heart morphogenesis. Single nucleotide polymorphisms of TGFBR3 have been found to be associated with gestational duration.…”

Section: Commentmentioning

confidence: 91%

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Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency

Govia

Birse

Sepehri

et al. 2018

American J Rep Immunol

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In this pilot study, significant differences in the amniotic fluid proteomic profiles in cases of cervical insufficiency compared to genetic amniocentesis were observed. Proteomic signatures were predictive of achieving latency > 1 week after diagnosis of cervical insufficiency. These preliminary findings suggest that proteomic analysis may be of value in predicting outcome following cervical insufficiency and warrants further validation in larger studies.

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Section: Commentmentioning

confidence: 60%

Section: Commentmentioning

confidence: 91%

Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency

Govia

Birse

Sepehri

et al. 2018

American J Rep Immunol

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“…The CVF proteome was analyzed in humans in various physiological conditions (1,2), mainly during pregnancy (3)(4)(5)(6)(7) or in several pathologies such as human papilloma virus (8) or candida albicans (9) infections. But few studies have investigated the CVF proteome during the cycle (10,11).…”

mentioning

confidence: 99%

Proteomes of the Female Genital Tract During the Oestrous Cycle

Soleilhavoup

Riou

Tsikis

et al. 2016

Molecular & Cellular Proteomics

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The success of fertilisation in mammals is linked to the correct migration of spermatozoa in the different compartments of the female genital tract and the adequate timing of their interaction with the female gamete. In many mammalian species including human, the deposition of semen in the vagina is followed by the sperm migration through the cervix, the uterus and then the oviduct before reaching the site of fertilisation. This sperm transit within the female tract includes mechanical and biochemical interactions with the luminal fluids leading to selection of spermatozoa able to fertilize the oocyte.The first physiological barrier the spermatozoa will have to go through is the cervix whose vaginal side is covered by a highly viscous mucus, the cervical vaginal fluid (CVF) 1 . The CVF proteome was analyzed in humans in various physiological conditions (1, 2), mainly during pregnancy (3-7) or in several pathologies such as human papilloma virus (8) or candida albicans (9) infections. But few studies have investigated the CVF proteome during the cycle (10, 11). The amount of CVF increases at the time of ovulation concomitantly with a higher state of hydration and a reduced viscosity, to facilitate sperm migration (12). The mechanical properties of the mucus are essential to select spermatozoa with the highest fertilizing ability, i.e. with normal morphology and efficient mobility. The main structural components of the cervical mucus are mucins, highly glycosylated, high-molecular-weight proteins assembled into a filamentous and viscous mesh (13). The transcription of the mucin genes is increased at oestrus in the bovine cervical epithelium (14). The amount of mucins and their level of glycosylation are expected to contribute to the viscosity of the mucus (11). Therefore, the quantification of proteins such From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements,

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“…Therefore, analysis of the CVF proteome can provide insights on the condition and functioning of the female reproductive system. Although many CVF proteomes have focused on pregnant women and aimed to discover biomarkers to predict preterm labor, the CVF proteome of healthy, nonpregnant women has received limited attention in comparison. Only three studies reported the CVF proteomes of healthy, nonpregnant women, which ranged in size from 59 to 685 proteins …”

Section: Introductionmentioning

confidence: 99%

Discovery of Antimicrobial Peptides in Cervical‐Vaginal Fluid from Healthy Nonpregnant Women via an Integrated Proteome and Peptidome Analysis

Muytjens

Diamandis

2017

Proteomics

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Cervical-vaginal fluid (CVF) covers the lower part of the female reproductive system and functions in the homeostasis and immunity of the surrounding tissues. In contrast to the CVF proteome of both nonpregnant and pregnant women, the CVF peptidome has not been reported to date. In the current study, we identified 1087 proteins in CVF, of which 801 proteins were not previously identified in CVF proteomes. The presence of the tissue-specific proteins oviductal glycoprotein 1 and tubulin polymerization-promoting protein family member 3 strongly suggests that the tissues of the upper female reproductive tract contribute to the protein composition of CVF. The tremendous catalytic potential of CVF was highlighted by the identification of 85 proteases and the detection of pH-dependent trypsin-like proteolytic activity. Over 1000 endogenous peptides were detected in the CVF peptidome, and 39 peptides are predicted to have antimicrobial activity. The detailed proteomic and peptidomic analysis of CVF will further aid in the delineation of pathways related to reproduction, immunity and host defense, and assist in developing new biomarkers for malignant and other diseases of the female reproductive tract. Data are available via ProteomeXchange with identifiers PXD004450 (CVF peptidome) and PDX004363 (CVF proteome).

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Proteomic analysis of cervical vaginal fluid proteins among women in recurrent preterm labor

Abstract: The lack of confirmation of potential biomarkers identified by mass spectrometry and ELISA demonstrates the challenges of validating PTB biomarkers and suggests that a panel of biomarkers would improve the predictive value of CVF testing.

Cited by 14 publications

References 25 publications

Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency

Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency

Proteomes of the Female Genital Tract During the Oestrous Cycle

Discovery of Antimicrobial Peptides in Cervical‐Vaginal Fluid from Healthy Nonpregnant Women via an Integrated Proteome and Peptidome Analysis

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