Discovery of Antimicrobial Peptides in Cervical‐Vaginal Fluid from Healthy Nonpregnant Women via an Integrated Proteome and Peptidome Analysis

Muytjens, Carla; Yu, Yifan; Diamandis, Eleftherios P.

doi:10.1002/pmic.201600461

Cited by 16 publications

(21 citation statements)

References 61 publications

(89 reference statements)

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“…Comparison of the obtained results with previously published CVF proteomes demonstrated the huge variability of the CVF proteome (Figure ). More than 50% of all proteins in the CVF studies on healthy nonpregnant women were similar to our NILM data.…”

Section: Resultsmentioning

confidence: 54%

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Label‐free cervicovaginal fluid proteome profiling reflects the cervix neoplastic transformation

et al. 2019

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Cervicovaginal fluid (CVF) is a valuable source of clinical information about the female reproductive tract in both nonpregnant and pregnant women. The aim of this study is to specify the CVF proteome at different stages of cervix neoplastic transformation by label-free quantitation approach based on liquid chromatography tandem mass spectrometry (LC-MS/MS) method. The proteome composition of CVF from 40 women of reproductive age with human papillomavirus (HPV)-associated cervix neoplastic transformation (low-grade squamous intraepithelial lesion [LSIL], high-grade squamous intraepithelial lesion [HSIL], and CANCER) was investigated. Hierarchical clustering and principal component analysis (PCA) of the proteomic data obtained by a label-free quantitation approach show the distribution of the sample set between four major clusters (no intraepithelial lesion or malignancy [NILM], LSIL, HSIL and CANCER) depending on the form of cervical lesion. Multisample ANOVA with subsequentWelch's t test resulted in 117 that changed significantly across the four clinical stages, including 27 proteins significantly changed in cervical cancer. Some of them were indicated as promising biomarkers previously (ACTN4, VTN, ANXA1, CAP1, ANXA2, and MUC5B). CVF proteomic data from the discovery stage were analyzed by the partial least squares-discriminant analysis (PLS-DA) method to build a statistical model, allowing to differentiate severe dysplasia (HSIL and CANCER) from the mild/normal stage (NILM and LSIL), and receiver operating characteristic (ROC) area under the curve (AUC) were obtained on an independent set of 33 samples. The sensitivity of the model was 77%, and the specificity was 94%; AUC was equal to 0.87. CVF proteome proved to be reflect the stage of cervical epithelium neoplastic process.

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Section: Resultsmentioning

confidence: 54%

“…Venn diagram of the overlap between cervicovaginal fluid (CVF) proteins identified in the current study and other publications. A, CVF proteome of healthy, HPV negative, nonpregnant women (no intraepithelial lesion or malignancy [NILM]) . B, Pathological CVF proteome: G.a.a.…”

Section: Resultsmentioning

confidence: 99%

Label‐free cervicovaginal fluid proteome profiling reflects the cervix neoplastic transformation

et al. 2019

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“…A total of 54 human proteins were found exclusively in the birth roll samples, compared to two in the control sample. These proteins were then compared to the proteome from cervico-vaginal fluid (CVF) published by Muytjens et al [47].…”

Section: Resultsmentioning

confidence: 99%

Girding the loins? Direct evidence of the use of a medieval parchment birthing girdle from biomolecular analysis

Fiddyment

Goodison

Brenner

et al. 2020

Preprint

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In this paper we describe a dry non-invasive extraction method to detect palaeoproteomic evidence from stained manuscripts. The manuscript analysed in this study is a medieval parchment birth girdle (Wellcome Collection Western MS. 632) made in England and thought to be used by pregnant women while giving birth. Using a dry non-invasive sampling method we were able to extract both human and non-human peptides from the stains, including evidence for the use of honey, cereals, ovicaprine milk and legumes. In addition, a large number of human peptides were detected on the birth roll, many of which are found in cervico-vaginal fluid. This suggests that the birth roll was actively used during childbirth. This study is the first to extract and analyse non-collagenous peptides from a parchment document using a dry non-invasive sampling method and demonstrates the potential of this type of analysis for stained manuscripts, providing direct biomolecular evidence for active use.

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“…All women had no cervical cancerous disease and abstained from sexual activity at least 3 days prior to sample collection. The samples of CVF were collected with a softcup collection device as described [11]. Then, the CVF samples were transferred into 50 mL conical centrifuge tube and were stored at −80°C until analysis.…”

Section: Collection Of Cervical-vaginal Fluid and Ethicsmentioning

confidence: 99%

Changes of miRNA Expression Profiles from Cervical-Vaginal Fluid-Derived Exosomes in Response to HPV16 Infection

Wang

et al. 2020

BioMed Research International

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As an oncogenic virus, HPV16 can lead to the dysfunction of cervical epithelial cells and contribute to the progression of cervical cancer. Components from the cervical-vaginal fluid (CVF) could be used as the basis for cervical cancer screening. Exosomes are widely present in various body fluids and participate in intercellular communication via its cargos of proteins, mRNAs, and miRNAs. This study was conducted to explore the changes of miRNAs in exosomes isolated form the cervical-vaginal fluid during HPV16 infection and to predict the potential effects of exosomal miRNAs on the development of cervical cancer. CVF was collected from volunteers with or without HPV16 infection. The exosomes in CVF were identified by electron microscopy. Microarray analysis was subjected to find the differentially expressed miRNAs in CVF exosomes. To confirm the results, 16 miRNAs were randomly selected to go through real-time quantitative polymerase chain reaction. In addition, GO and pathway analyses were conducted to reveal potential functions of differentially expressed miRNAs. A total of 2548 conserved miRNAs were identified in the cervical-vaginal fluid-derived exosomes. In response to HPV16 infection, 45 miRNAs are significantly upregulated and 55 miRNAs are significantly downregulated (P<0.05). The GO and KEGG pathway analyses revealed that these differentially expressed miRNAs are tightly associated with cervical cancer tumorigenesis, through interaction with the Notch signaling pathway, TNF signaling pathway, and TGF-β signaling pathway. These results suggest that exosomal miRNAs in CVF are differentially expressed in HPV16 infection patients and HPV16-free volunteers. It provided a novel insight to understand the underlying mechanism of HPV16 infection in regulating cervical cancer progression.

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Discovery of Antimicrobial Peptides in Cervical‐Vaginal Fluid from Healthy Nonpregnant Women via an Integrated Proteome and Peptidome Analysis

Cited by 16 publications

References 61 publications

Label‐free cervicovaginal fluid proteome profiling reflects the cervix neoplastic transformation

Label‐free cervicovaginal fluid proteome profiling reflects the cervix neoplastic transformation

Girding the loins? Direct evidence of the use of a medieval parchment birthing girdle from biomolecular analysis

Changes of miRNA Expression Profiles from Cervical-Vaginal Fluid-Derived Exosomes in Response to HPV16 Infection

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