Aligning cells in arbitrary directions on a membrane sheet using locally formed microwrinkles

Saitō, Akira; Matsui, Tsubasa S.; Sato, Masaaki; Deguchi, Shinji

doi:10.1007/s10529-013-1368-2

Cited by 5 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This leads to a mild topography with a continuous change in height that is distinct from prior studies with discontinuous step changes in structure [38][39][40][41][42][43][44]. Surface wrinkles, on the other hand, also provide a small and continuous surface topography but are limited to sinusoidal geometries [45][46][47].…”

Section: Fabrication Of Surfacesmentioning

confidence: 83%

Guiding cell migration with microscale stiffness patterns and undulated surfaces

et al. 2016

View full text Add to dashboard Cite

Section: Fabrication Of Surfacesmentioning

confidence: 83%

Guiding cell migration with microscale stiffness patterns and undulated surfaces

et al. 2016

View full text Add to dashboard Cite

“…From the 1-D stretching experiment, we know that the wrinkles were perpendicular to the direction of compressive stress. Based on this principle, we produced ring wrinkles using radial stresses [ 26 , 27 ]. In our experiment, ring wrinkles were achieved by pulling the center of a freely hanging PDMS film, as shown in Figure 4 .…”

Section: Resultsmentioning

confidence: 99%

Ring Wrinkle Patterns with Continuously Changing Wavelength Produced Using a Controlled-Gradient Light Field

Sheng

et al. 2018

Materials

View full text Add to dashboard Cite

We report a facile method to prepare gradient wrinkles using a controlled-gradient light field. Because of the gradient distance between the ultraviolet (UV) lamp and polydimethylsiloxane (PDMS) substrate during UV/ozone treatment, the irradiance reaching the substrate continuously changed, which was transferred into the resulting SiOx film with a varying thickness. Therefore, wrinkles with continuously changing wavelength were fabricated using this approach. It was found that the wrinkle wavelength decreased as the distance increased. We fabricated 1-D wrinkle patterns and ring wrinkles with a gradient wavelength. The ring wrinkles were prepared using radial stresses, which were achieved by pulling the center of a freely hanging PDMS film. The resulting wrinkles with changing wavelength can be used in fluid handling systems, biological templates, and optical devices.

show abstract

“…Therefore, we will try to add powder constituents to cubic eggshells in order to increase the viability of chick embryos. In addition, by fabricating the small patterns on the surface of the cubic eggshell using microfabrication techniques [ 55 – 57 ], the release ratio of the mixed constituents can be designed and controlled.…”

Section: Discussionmentioning

confidence: 99%

Egg-in-Cube: Design and Fabrication of a Novel Artificial Eggshell with Functionalized Surface

2015

View full text Add to dashboard Cite

An eggshell is a porous microstructure that regulates the passage of gases to allow respiration. The chick embryo and its circulatory system enclosed by the eggshell has become an important model for biomedical research such as the control of angiogenesis, cancer therapy, and drug delivery test, because the use of embryo is ethically acceptable and it is inexpensive and small. However, chick embryo and extra-embryonic blood vessels cannot be accessed freely and has poor observability because the eggshell is tough and cannot be seen through, which limits its application. In this study, a novel artificial eggshell with functionalized surface is proposed, which allows the total amount of oxygen to pass into the egg for the chick embryo culturing and has high observability and accessibility for embryo manipulation. First, a 40-mm enclosed cubic-shaped eggshell consisting of a membrane structure and a rigid frame structure is designed, and then the threshold of the membrane thickness suitable for the embryo survival is figured out according to the oxygen-permeability of the membrane structure. The designed artificial eggshell was actually fabricated by using polydimethylsiloxane (PDMS) and polycarbonate (PC) in the current study. Using the fabricated eggshell, chick embryo and extra-embryonic blood vessels can be observed from multiple directions. To test the effectiveness of the design, the cubic eggshells were used to culture chick embryos and survivability was confirmed when PDMS membranes with adequate oxygen permeability were used. Since the surface of the eggshell is transparent, chick embryo tissue development could be observed during the culture period. Additionally, the chick embryo tissues could be accessed and manipulated from outside the cubic eggshell, by using mechanical tools without breakage of the eggshell. The proposed “Egg-in-Cube” with functionalized surface has great potential to serve as a promising platform for biomedical research.

show abstract

Aligning cells in arbitrary directions on a membrane sheet using locally formed microwrinkles

Cited by 5 publications

References 20 publications

Guiding cell migration with microscale stiffness patterns and undulated surfaces

Guiding cell migration with microscale stiffness patterns and undulated surfaces

Ring Wrinkle Patterns with Continuously Changing Wavelength Produced Using a Controlled-Gradient Light Field

Egg-in-Cube: Design and Fabrication of a Novel Artificial Eggshell with Functionalized Surface

Contact Info

Product

Resources

About