Guiding cell migration with microscale stiffness patterns and undulated surfaces

Pham, Jonathan T.; Xue, Longjian; Campo, Aránzazu del; Salierno, Marcelo

doi:10.1016/j.actbio.2016.04.031

Cited by 30 publications

(33 citation statements)

References 60 publications

(105 reference statements)

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“…Interestingly, while the machining marks were obvious on the machined implant, they were less evident on the complex surface. There have been numerous reports of cell migration along the long axes of surfaces with linear features 40,41 , but we saw no evidence that these topographic features influenced the directional growth of vessels.…”

Section: Discussioncontrasting

confidence: 84%

Nanosurfaces modulate the mechanism of peri-implant endosseous healing by regulating neovascular morphogenesis

et al. 2018

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Nanosurfaces have improved clinical osseointegration by increasing bone/implant contact. Neovascularization is considered an essential prerequisite to osteogenesis, but no previous reports to our knowledge have examined the effect of surface topography on the spatio-temporal pattern of neovascularization during peri-implant healing. We have developed a cranial window model to study peri-implant healing intravitally over clinically relevant time scales as a function of implant topography. Quantitative intravital confocal imaging reveals that changing the topography (but not chemical composition) of an implant profoundly affects the pattern of peri-implant neovascularization. New vessels develop proximal to the implant and the vascular network matures sooner in the presence of an implant nanosurface. Accelerated angiogenesis can lead to earlier osseointegration through the delivery of osteogenic precursors to, and direct formation of bone on, the implant surface. This study highlights a critical aspect of peri-implant healing, but also informs the biological rationale for the surface design of putative endosseous implant materials.

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Section: Discussioncontrasting

confidence: 84%

Nanosurfaces modulate the mechanism of peri-implant endosseous healing by regulating neovascular morphogenesis

et al. 2018

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“…Polydimethylsiloxane (PDMS)‐based elastomers are found in a host of applications, from adhesives and biomaterials to soft robotics and three‐dimensional printing . These elastomers are also common in fundamental research on soft matter physics and mechanics.…”

Section: Introductionmentioning

confidence: 99%

“…For example, studies on soft adhesion, wetting, cavitation, and cell–surface interactions frequently implement PDMS as a model material. PDMS is used because it is commercially available (e.g., Sylgard 184), easy to prepare, and easy to tune the elastic modulus from a few megapascal down to a few kilopascal . This simple modulus control has enabled the growing and vast use of commercial PDMS elastomers with moduli below 100 kPa .…”

Section: Introductionmentioning

confidence: 99%

Extracting uncrosslinked material from low modulus sylgard 184 and the effect on mechanical properties

Glover

McLaughlin

McFarland

et al. 2020

Journal of Polymer Science

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Commercial silicone elastomers are commonly used in soft materials research due to their easily tunable mechanical properties.However, conventional polydimethylsiloxane (PDMS) elastomers with moduli below $100 kPa contain uncrosslinked free molecules, which play a significant role in their behavior. To utilize these materials, it is important to quantify what role these free molecules play in the mechanical response before and after their removal. We present a simple and inexpensive extraction method that enables the removal of free molecules from a lightly crosslinked sheet of Sylgard 184, a commercially available PDMS elastomer. The materials can contain a majority of free molecules yet maintain a thin and flat geometry without fractures after extraction. Subsequently, we compare the modulus, maximum stretchability, and hysteresis behavior with mixing ratios ranging from 60:1 to 30:1, before and after extraction. We show that the modulus, maximum stretchability, and dissipation increase upon extraction. Moreover, our approach offers a route to prepare crosslinked silicone elastomers with a modulus as low as $20 kPa without free molecules from a commercially available kit.

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“…Inspired by durotaxis in biology, solid substrate durotaxis has emerged as an attractive research field as the motion of nanoscale objects can be guided by substrate stiffness without the requirement for an energy source with implications for nanoscale actuation and energy conversion [29][30][31][32][33]. Moreover, solid substrate durotaxis, which was investigated by using computer simulation in the context of a flake sliding on a graphene substrate with a stiffness gradient, shares similarities with the stiffness-guided directional motion in living cells as in both cases weak van der Waals interactions are present [30].…”

Section: Introductionmentioning

confidence: 99%

Stiffness-guided motion of a droplet on a solid substrate

Theodorakis

Егоров

Milchev

2017

The Journal of Chemical Physics

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A range of technologies require the directed motion of nanoscale droplets on solid substrates. A way of realizing this effect is durotaxis, whereby a stiffness gradient of a substrate can induce directional motion without requiring an energy source. Here, we report on the results of extensive molecular dynamics investigations of droplets on a surface with varying stiffness. We find that durotaxis is enhanced by increasing the stiffness gradient and, also, by increased wettability of the substrate, in particular, when droplet size decreases. We anticipate that our study will provide further insights into the mechanisms of nanoscale directional motion.

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Guiding cell migration with microscale stiffness patterns and undulated surfaces

Cited by 30 publications

References 60 publications

Nanosurfaces modulate the mechanism of peri-implant endosseous healing by regulating neovascular morphogenesis

Nanosurfaces modulate the mechanism of peri-implant endosseous healing by regulating neovascular morphogenesis

Extracting uncrosslinked material from low modulus sylgard 184 and the effect on mechanical properties

Stiffness-guided motion of a droplet on a solid substrate

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