Identification of a locus characteristic of male individuals of buffalo grass [Buchloe dactyloides (Nutt.) Engelm.] by using an RAPD marker

Abstract

ABSTRACT. Buffalo grass [Buchloe dactyloides (Nutt.) Engelm.] plants can be either male, female, or hermaphrodite (monoecious). As there is no morphological difference in the early vegetative growth of these three classes of plants, it is worthwhile to use molecular biological methods to attempt to identify the sex of a plant at this early growth period. In this study, we identified 23 plants that had a stable sex for over at least 3 years. Of these, 9 were male plants, 10 were female plants, and 4 were hermaphrodites. Screening of 300 RAPD primers identified a primer, namely S211 (5'-ttccccgcga-3'), which is capable of identifying male plants. The specific fragment was cloned, sequenced, and submitted to the GenBank database Identification of buffalo grass male individuals using RAPD (accession No. JN982469). When used to identify the sex of 188 plants during their first growing season, the S211 primer correctly identified 85.8% of all male plants. Our results showed that the S211 primer can identify the male, and in doing so, it facilitates buffalo grass breeding work.

Sex differential marker FD for rapid sex identification of Litsea cubeba

“…In addition to morphological or chemical methodologies, molecular technologyis often used to study sex determination (Li et al, 2013) because it is an easy, rapid, and reliable solution that is not influenced by external factors. For example, Sarmahand Sarma (2011) identified intersimple sequence repeat markersthat were linked to sex determination in Calamustenuis (Roxb.)…”