No Association ofIFNG+874T/ASNP andNOS2A-954G/CSNP Variants with Nitric Oxide Radical Serum Levels or Susceptibility to Tuberculosis in a Brazilian Population Subset

Abstract

Tuberculosis (TB) is one of the most common infectious diseases in the world. Mycobacterium tuberculosis infection leads to pulmonary active disease in approximately 5–10% of exposed individuals. Both bacteria- and host-related characteristics influence latent infection and disease. Host genetic predisposition to develop TB may involve multiple genes and their polymorphisms. It was reported previously that interferon gamma (IFN-γ) and nitric oxide synthase 2 (NOS2) are expressed on alveolar macrophages from TB patients and are responsible for bacilli control; thus, we aimed this study at genotyping single nucleotide polymorphisms IFNG+874T/A SNP and NOS2A-954G/C SNP to estimate their role on TB susceptibility and determine whether these polymorphisms influence serum nitrite and NOx − production. This case-control study enrolled 172 TB patients and 179 healthy controls. Neither polymorphism was associated with susceptibility to TB. NOS2A-954G/C SNP was not associated with serum levels of nitrite and NOx −. These results indicate that variants of IFNG+874T/A SNP and NOS2A-954G/C SNP do not influence TB susceptibility or the secretion of nitric oxide radicals in the study population.

IL10A genotypic association with decreased IL-10 circulating levels in malaria infected individuals from endemic area of the Brazilian Amazon

“…In Brazil, the NOS2A-954G/C polymorphism have been reported in studies with tuberculosis and leprosy. In both studies, the allelic and genotypic frequencies were similar to the one found in this study, even though their population were from South and Southeast region [ 50 , 51 ]. Although no association has been found, this study is the first to report NOS2A-954G/C polymorphism and NO levels in malaria exposed individuals in endemic region of Brazil.…”

Association study of single nucleotide polymorphism of human Toll like receptor 9 and susceptibility to pulmonary tuberculosis in Egyptian population

“…In spite of the interaction of Mtb with TLRs leads to phagocyte activation, the interaction itself does not lead to immediate ingestion of the mycobacteria (Kleinnijenhuis et al, 2011). Host genetic may involve multiple genes and their polymorphisms to develop TB (Leandro et al, 2013). TLR association may differ from population to another.…”

Relationship of bovine <i>NOS2</i> gene polymorphisms to the risk of bovine tuberculosis in Holstein cattle

“…Mycobacterium bovis ( M. bovis ) is the main causative agent of bovine tuberculosis (bTB), a disease of great concern in many countries, including England, Ireland, Brazil, China and Taiwan, for its detrimental economic impact and its effect on humans [ 3 , 4 , 8 , 13 , 16 , 18 ]. The worldwide prevalence of bTB remains a significant economic burden, with annual losses of approximately 3 billion US dollars [ 1 ].…”

“…Previous studies suggest that certain host genetic variants contribute to tuberculosis susceptibility [ 1 , 2 , 3 , 4 ]. Several candidate proteins associated with the susceptibility, including nitric oxide synthase 2 inducible gene ( NOS2 ), tumor necrosis factor α ( TNF-α ), solute carrier family 11 member 1 ( SLC11A1 ), vitamin D receptor ( VDR ) and toll-like receptors ( TLRs ), have been investigated for their possible associations between genetic variants and tuberculosis susceptibility [ 11 , 15 , 16 , 18 , 19 , 23 , 31 , 35 ]. The inducible nitric oxide synthase (iNOS) which is encoded by the NOS2 gene is a cytoplasmic protein and absent in resting cells, but is rapidly produced in response to stimuli, such as infections and cytokines [ 6 , 12 , 21 ].…”