Identification and analysis of absorbed components and their metabolites in rat plasma and tissues after oral administration of ‘Ershiwuwei Shanhu’ pill extracts by UPLC-DAD/Q-TOF-MS

Ying, Xuhui; Liu, Mingying; Liang, Qionglin; Jiang, Min; Wang, Yiming; Fukai, Huang; Xie, Yanjun; Shao, Jiahao; Bai, Gang; Luo, Guangsheng

doi:10.1016/j.jep.2013.08.046

Cited by 31 publications

(33 citation statements)

References 32 publications

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“…In the MS 2 spectrum, the ion at m/z 353 generated the ion at m/z 191 [M − H − caffeoyl group] − , owing to the loss of a caffeoyl moiety. It was identified as chlorogenic acid in accordance with the previous data in literatures (Santos et al, ; Ying et al, ).…”

Section: Resultssupporting

confidence: 88%

Chemical identification and quality evaluation of Lycopus lucidus Turcz by UHPLC‐Q‐TOF‐MS and HPLC‐MS/MS and hierarchical clustering analysis

Ren

Ding

Sun

et al. 2016

Biomedical Chromatography

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Lycopus lucidus Turcz has been used as a traditional phytomedicine for menstrual disorder, amenorrhea, menstrual cramps, inflammation and cardiovascular diseases. However, there is not enough information about identification and quantification for the chemical constituents of L. lucidus Turcz. In this work, a simple, rapid and sensitive UHPLC-Q-TOF-MS method was developed for characterization and identification of the phytochemical compositions in L. lucidus Turcz in negative ion mode. A total of 37 compounds, including 15 phenolic acids, 12 flavonoids, three triterpenoids and seven organic acids were tentatively characterized and identified by means of the retention time, accurate mass and characteristic fragment ions. Thirteen compounds were reported for the first time in L. lucidus Turcz. Among of them, 11 compounds were further quantified by multiple reactions monitoring. The results showed good performance with respect to linearity (r > 0.9959), repeatability (RSD < 2.6%), intra- and inter-day precision (RSD < 3.2%), recovery (93.1-104.9%), and lower limit of quantification (5-50 ng/mL). Subsequently, the results were analyzed and classified by hierarchical cluster analysis. The research could be applied for identification and quality evaluation for L. lucidus Turcz.

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Section: Resultssupporting

confidence: 88%

Chemical identification and quality evaluation of Lycopus lucidus Turcz by UHPLC‐Q‐TOF‐MS and HPLC‐MS/MS and hierarchical clustering analysis

Ren

Ding

Sun

et al. 2016

Biomedical Chromatography

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“…Similarly, the MS characteristics of kaempferol‐type flavonoid‐ O ‐glycosides (peak 45) were also identified . Peak 24 showed a loss of glucosyl (162 Da) in positive ion mode, and generated an obvious fragment ion at m/z 491 in negative ion mode because of 4‐vinylphenol loss , while compound 58 exhibited a 162 Da loss of a glucosyl group and gave an aglycone ion ([M+H‐glu] + ) at m/z 269. Both of them were definitely identified as HSYA and Ononin by comparison with the authentic standard, respectively.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination and qualitative analysis of six types of components in Naoxintong capsule by miniaturized matrix solid‐phase dispersion extraction coupled with ultra high‐performance liquid chromatography with photodiode array detection and quadrupole time‐of‐flight mass spectrometry

Wang

Jiang

Ding

et al. 2018

J of Separation Science

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A simple and effective sample preparation process based on miniaturized matrix solid-phase dispersion was developed for simultaneous determination of phenolic acids (gallic acid, chlorogenic acid, ferulic acid, 3,5-dicaffeoylqunic acid, 1,5-dicaffeoylqunic acid, rosmarinic acid, lithospermic acid, and salvianolic acid B), flavonoids (kaempferol-3-O-rutinoside, calycosin, and formononetin), lactones (ligustilide and butyllidephthalide), monoterpenoids (paeoniflorin), phenanthraquinones (cryptotanshinone), and furans (5-hydroxymethylfurfural) in Naoxintong capsule by ultra high-performance liquid chromatography. The optimized condition was that 25 mg Naoxintong powder was blended homogeneously with 100 mg Florisil PR for 4 min. One milliliter of methanol/water (75:25, v/v) acidified by 0.05% formic acid was selected to elute all components. It was found that the recoveries of the six types of components ranged from 61.36 to 96.94%. The proposed miniaturized matrix solid-phase dispersion coupled with ultra high-performance liquid chromatography was successfully applied to simultaneous determination of the six types of components in Naoxintong capsules. The results demonstrated that the proposed miniaturized matrix solid-phase dispersion coupled with ultra high-performance liquid chromatography could be used as an environmentally friendly tool for the extraction and determination of multiple bioactive components in natural products.

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“…Compounds P10, P14, P16, P18, P23, P32, P33, P39, and P41 were confirmed by authentic standards and identified as hyperoside, rutin, isoquercitrin, astragalin, luteoloside, quercetin, luteolin, kaempferol, and isorhamnetin. And flavonoid glycosides were characterized according to form the prominent ion of [M+H‐162] + or [M+H‐146] + in their MS 2 spectra, indicating loss of glucose or rhamnose residue .…”

Section: Resultsmentioning

confidence: 99%

Rapid characterization of the absorbed chemical constituents of Tangzhiqing formula following oral administration using UHPLC–Q‐TOF‐MS

Xin-ze

et al. 2018

J of Separation Science

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Tangzhiqing formula, a Chinese herbal formula, is used for the treatment of type II diabetes and prediabetes. Although its effectiveness has been certified by clinical use, its absorbed chemical constituents are not comprehensively represented. Thence, in order to reveal potential bioactive components and metabolism of Tangzhiqing formula, an ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry method was developed. A total of 86 absorbed components, including 38 prototype compounds and 48 metabolites, were identified in rat plasma, urine, and feces after oral administration of Tangzhiqing formula. This was the first systematic study on the chemical constituents and metabolic profiling of Tangzhiqing formula. The results indicated that alkaloids and flavonoids were main absorbed components, and glucuronidation and sulfation were the major metabolites. Moreover we concluded that alkaloids and flavonoids first underwent demethylation and hydrolysis reactions before biotransformed to phase II metabolites. This study provided valuable data for safety estimation of Tangzhiqing formula, which will be advantageous for clinical application.

show abstract

Identification and analysis of absorbed components and their metabolites in rat plasma and tissues after oral administration of ‘Ershiwuwei Shanhu’ pill extracts by UPLC-DAD/Q-TOF-MS

Cited by 31 publications

References 32 publications

Chemical identification and quality evaluation of Lycopus lucidus Turcz by UHPLC‐Q‐TOF‐MS and HPLC‐MS/MS and hierarchical clustering analysis

Chemical identification and quality evaluation of Lycopus lucidus Turcz by UHPLC‐Q‐TOF‐MS and HPLC‐MS/MS and hierarchical clustering analysis

Rapid characterization of the absorbed chemical constituents of Tangzhiqing formula following oral administration using UHPLC–Q‐TOF‐MS

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