Antioxidant Capacity of the Leaf Extract Obtained from Arrabidaea chica Cultivated in Southern Brazil

Abstract

Arrabidaea chica leaf extract has been used by people as an anti-inflammatory and astringent agent as well as a remedy for intestinal colic, diarrhea, leucorrhea, anemia, and leukemia. A. chica is known to be a good producer of phenolics. Therefore, in the present study, we investigated its antioxidant activity. The phenolic composition of A. chica leaves was studied by liquid chromatography coupled to diode array detection (LC–DAD) and liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS), and isoscutellarein, 6-hydroxyluteolin, hispidulin, scutellarein, luteolin, and apigenin were identified. The extract from leaves of A. chica was tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, β-carotene bleaching test, and total reactive antioxidant potential (TRAP) method. The crude extract quenched DPPH free radicals in a dose-dependent manner, and the IC50 of the extract was 13.51 µg/mL. The β-carotene bleaching test showed that the addition of the A. chica extract in different concentrations (200 and 500 µg/mL) prevented the bleaching of β-carotene at different degrees (51.2% ±3.38% and 94% ±4.61%, respectively). The TRAP test showed dose-dependent correlation between the increasing concentrations of A. chica extract (0.1, 0.5, and 1.0 µg/mL) and the TRAP values obtained by trolox (hydro-soluble vitamin E) 0.4738±0.0466, 1.981±0.1603, and 6.877±1.445 µM, respectively. The 2 main flavonoids, scutellarein and apigenin, were separated, and their antioxidant activity was found to be the same as that of the plant extract. These 2 flavonoids were quantified in the plant extract by using a validated HPLC-UV method. The results of these tests showed that the extract of A. chica had a significant antioxidant activity, which could be attributed to the presence of the mixture of flavonoids in the plant extract, with the main contribution of scutellarein and apigenin.

Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

“…during the tendon healing process. 4 Antimicrobial, 5 antiinflammatory, 6 and antioxidant 7 potentials of this species have also been reported.…”

Pharmacologically active flavonoids from the anticancer, antioxidant and antimicrobial extracts of Cassia angustifolia Vahl

“…Phenolics and flavonoids are secondary metabolites derived from tyrosine and phenylalanine with potent antibacterial and antioxidant activities [34]. It was reported that isolated flavonoids quercimeritrin ( 1 ), scutellarein ( 2 ), and rutin ( 3 ) have significant antioxidant activities against oxidative stress [35–37]. Note that all three have 1,2-dihydroxybenzene groups which are readily oxidized to orthoquinones, making them strong antioxidants.…”

Ethnobotanical study of medicinal plants by population of Valley of Juruena Region, Legal Amazon, Mato Grosso, Brazil

“…In addition, isoscutellarein, 6-hydroxyluteolin, hispidulin, scutellarein luteolin, and apigenin have been reported from this plant, while 4'-hydroxy-3,7-dimethoxyflavone, vicenin-2 and kaempferol have been isolated from its leaves (Barbosa et al, 2008;Siraichi et al, 2013). There are reports of the antiinflammatory, anti-allergic, and anti-tumor properties of these compounds in the literature (Eda et al, 2002;Hirano et al, 2001;Seelinger et al, 2008).…”