1997
DOI: 10.1016/s0076-6879(97)78026-1
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[24] Fluorescence probes for studying membrane heterogeneity

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Cited by 43 publications
(28 citation statements)
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“…Free-zone electrophoresis which was introduced first to investigate the charge density (6) is an alternative method (7); however, this technique entails a long procedure and is limited to the observation of the homogeneity of charged components. The use of fluorescent probes can be applied to evaluate several kinds of homogeneity (8). However, it is suspect whether the probes themselves are distributed homogeneously in some cases, and this method cannot be adopted for quality control because there are few cases in which guest molecules in the products can be treated as fluorescent probes.…”
Section: Introductionmentioning
confidence: 99%
“…Free-zone electrophoresis which was introduced first to investigate the charge density (6) is an alternative method (7); however, this technique entails a long procedure and is limited to the observation of the homogeneity of charged components. The use of fluorescent probes can be applied to evaluate several kinds of homogeneity (8). However, it is suspect whether the probes themselves are distributed homogeneously in some cases, and this method cannot be adopted for quality control because there are few cases in which guest molecules in the products can be treated as fluorescent probes.…”
Section: Introductionmentioning
confidence: 99%
“…The use of charged but neutral probes (having both electron donor and acceptor groups; e.g., prodan) or uncharged probes (e.g., DPH; Davenport, 1997) may circumvent this problem. However, the nonpolar nature of DPH restricts its solubility in aqueous systems and thus limits its use as a probe for protein solutions.…”
Section: Comparison Of Different Methodsmentioning
confidence: 99%
“…The idea of tryptophan imaging of membrane proteins has been proposed and analyzed by Kleinfeld [67]: tryptophans in membrane proteins serve as donors and anthroyloxy fluorophores serve as acceptors with the anthroyloxy group attached to lipids at various distances from the midplane of the membrane. Fluorescence studies of membrane heterogeneity has been reviewed by Davenport [76]. Other interesting membrane FRET studies are mentioned in Refs [70][71][72][73][74][75].…”
Section: Fret Theory 1965-2012mentioning
confidence: 99%