High pressure nanoelectrospray ionization mass spectrometry for analysis of aqueous solutions

Rahman, Md. Matiur; Mandal, Mridul Kanti; Hiraoka, Kenzo; Chen, Lee Chuin

doi:10.1039/c3an00699a

Cited by 23 publications

(31 citation statements)

References 41 publications

(46 reference statements)

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“…A series of high pressure ion sources had been developed in our laboratory to improve the performance of ESI [29,30], nanoESI [31], and atmospheric pressure chemical ionization (APCI) [32]. It had also been used to implement field desorption (FD), an ESI-like ionization from molten sample without the use of any solvent [33].…”

Section: Introductionmentioning

confidence: 99%

Rapid Online Non-Enzymatic Protein Digestion Analysis with High Pressure Superheated ESI-MS

Chen

Kinoshita

Noda

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Recently, we reported a new ESI ion source that could electrospray the super-heated aqueous solution with liquid temperature much higher than the normal boiling point (J. Am. Soc. Mass Spectrom. 25, 1862-1869). The boiling of liquid was prevented by pressurizing the ion source to a pressure greater than atmospheric pressure. The maximum operating pressure in our previous prototype was 11atm, and the highest achievable temperature was 180°C. In this paper, a more compact prototype that can operate up to 27atm and 250°C liquid temperatures is constructed, and reproducible MS acquisition can be extended to electrospray temperatures that have never before been tested. Here, we apply this super-heated ESI source to the rapid online protein digestion MS. The sample solution is rapidly heated when flowing through a heated ESI capillary, and the digestion products are ionized by ESI in situ when the solution emerges from the tip of the heated capillary. With weak acid such as formic acid as solution, the thermally accelerated digestion (acid hydrolysis) has the selective cleavage at the aspartate (Asp, D) residue sites. The residence time of liquid within the active heating region is about 20s. The online operation eliminates the need to transfer the sample from the digestion reactor, and the output of the digestive reaction can be monitored and manipulated by the solution flow rate and heater temperature in a near real-time basis.

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Section: Introductionmentioning

confidence: 99%

Rapid Online Non-Enzymatic Protein Digestion Analysis with High Pressure Superheated ESI-MS

Chen

Kinoshita

Noda

et al. 2015

J. Am. Soc. Mass Spectrom.

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“…25) Super-atmospheric pressure ESI has been demonstrated with ESI 26) as well as nanoESI. 27,28) For such a high pressure ESI, the ion source consisting of an ESI emitter is placed in- side a chamber lled with air or nitrogen gas. High pressure ESI is particularly useful when the spray solvent is completely aqueous and also when it is required to heat samples higher than 100°C.…”

Section: High Pressure Esimentioning

confidence: 99%

Electrospray Modifications for Advancing Mass Spectrometric Analysis

Meher

Chen

2017

Mass Spectrometry

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Generation of analyte ions in gas phase is a primary requirement for mass spectrometric analysis. One of the ionization techniques that can be used to generate gas phase ions is electrospray ionization (ESI). ESI is a so ionization method that can be used to analyze analytes ranging from small organics to large biomolecules. Numerous ionization techniques derived from ESI have been reported in the past two decades.ese ion sources are aimed to achieve simplicity and ease of operation. Many of these ionization methods allow the exibility for elimination or minimization of sample preparation steps prior to mass spectrometric analysis. Such ion sources have opened up new possibilities for taking scienti c challenges, which might be limited by the conventional ESI technique. us, the number of ESI variants continues to increase. is review provides an overview of ionization techniques based on the use of electrospray reported in recent years. Also, a brief discussion on the instrumentation, underlying processes, and selected applications is also presented.

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“…Based on the design of second ESI prototype, a number of super-atmospheric pressure ion sources has been constructed to implement nanoESI, 15) probe ESI, 15) corona discharged ionization, 17) and non-vacuum eld desorption. 18) Our recent prototype was used in a relatively harsh experimental condition with pressure up to 30 atm and temperature over 200°C.…”

Section: Ion Source For Superheated Esimentioning

confidence: 99%

“…11a, the protein solvent is 2 v/v% formic acid aqueous solution which has recently become a standard reagent for the chemical digestion. Except for the peptide fragment [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18], all other hydrolysis products are due to the cleavage at the Asp sites. Comparison was also done with acetic acids (Fig.…”

Section: Rapid In-situ Protein Digestion Ms With Superheated Esimentioning

confidence: 99%

“…We have implemented the super-atmospheric pressure ionization MS with pure electrospray, [12][13][14][15][16] corona discharge based chemical ionization, 17) and eld desorption, 18) and had routinely run our mass spectrometer with ion source pressures ranging from several to several tens of atm. For typical analytical application where the solvent composition can be ne-tuned to best-suit the API-ESI, it is unnecessary to pressurize the ion source, though it is possible to get some signal enhancement due to higher gas throughput.…”

Section: Introductionmentioning

confidence: 99%

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