Development of a high-throughput ultra performance liquid chromatography–mass spectrometry assay to profile 18 eicosanoids as exploratory biomarkers for atherosclerotic diseases

Metabolomics approach to assessing plasma 13- and 9-hydroxy-octadecadienoic acid and linoleic acid metabolite responses to 75-km cycling

“…13-HODE ϩ 9-HODE are monohydroxy lipoxygenation products and the most widely distributed of linoleic acid metabolites (17,32). The HODEs are secreted by a variety of cells including macrophages, endothelial cells, platelets, and smooth muscle cells, and exert biological and signaling activities as PPAR and GPR132 ligands (12,21,28,37). Cell injury activates lipoxygenases and may be one pathway through which intensive exercise increases production of HODES (33).…”

“…Plasma levels of 13-HODE ϩ 9-HODE are responsive to lifestyle interventions, with decreases reported when subjects adopt healthy diets and lose excess body weight (2,3,7,25). 13-HODE ϩ 9-HODE are generated through the 15-lipoxygenase-1 (15-LOX) pathway in a variety of cell types (17,25), are ligands of peroxisome proliferator-activated receptors (PPARs) (28), and can act through G protein-coupled receptor 132 (GPR132) to exert pro-inflammatory effects (37).…”

A Simple and Highly Sensitive Quantitation of Eicosanoids in Biological Samples Using Nano-flow Liquid Chromatography/ Mass Spectrometry

“…In a previous report, the recovery rates of eicosanoid by LLE were 10.61% for TXB2, 25.56% for 11-dehydroxy TXB2, 35.19% for PGF2α, 33.21% for PGD2, 49.19% for PGE2 and 12.75% for 6-keto PGF1α extracted with two-step LLE (the first extraction was chloroform/isopropanol (2:1); the second extraction was with methyl tert-butyl ether) 32 and 22.1% for PGD2, 26.5% for PGE2, 21.1% for LTB4 and 29.5% for PGF2α when extracted with 1 M acetic acid/2-isopropanol/hexane (2:20:30, v/v/v). 23 As for the recovery rate of eicosanoid using a SPE column, more than 65% by weak anion-exchange, 0 -54% by strong anion-exchange and more than 68% by octadecylsilyl based column was reported. 12 On the other hand, the recovery rate of our result was shown to be equal or above that of the SPE and LLE method.…”

“…Solid-phase extraction (SPE) with reverse phase, 12,18,19 strong anion exchange, 20 and strong cation exchange 21,22 is a key method for the selective extraction and condensation of target molecules. Liquid-liquid extraction (LLE) 12,23 is also frequently used for metabolite fractionation by solvent partitioning and protein removal. These processes largely contribute to the enrichment of target molecules and the removal of abundant interferences, enabling efficient measurements of trace metabolites in combination with LC/MS/MS analysis.…”

Thyroxine therapy ameliorates serum levels of eicosanoids in Chinese subclinical hypothyroidism patients

“…Hence, the increased ratio of 11-dehydroTXB2/6-Keto PGF1a in hypothyroid patients may represent a shift toward vasoconstriction. Numerous studies have shown that many eicosanoids play key roles in the development of atherosclerosis and are new biomarkers and drug therapy targets [20][21][22][23] . Therefore, whether the eicosanoid profile in SH patients is abnormal and associated with susceptibility to cardiovascular, cerebrovascular and other serious diseases and whether L-T4 replacement therapy (LTR) can affect the eicosanoid profile need to be studied further.…”