Oxygen‐rich coating promotes binding of proteins and endothelialization of polyethylene terephthalate polymers

Abstract

The formation of endothelial cell monolayer on prosthetic implants has not sufficiently explored. The main reasons leading to the development of thrombosis and/or intimal hyperplasia is the lack of endothelialization. In the present work, we have studied the influence of oxygen and fluorine plasma treatment of polyethylene terephthalate (PET) polymers on human microvascular endothelial cell adhesion and proliferation. We characterized the polymer surface, wettability, and oxidation potential upon plasma treatment. Moreover, binding of serum and media compounds on PET surface was monitored by Quartz crystal microbalance method, X-ray photoelectron spectroscopy, and atomic force microscopy. Cell adhesion and morphology was assessed by light and scanning electron microscopy. The influence of plasma treatment on induction of cellular oxidative stress and cell proliferation was evaluated. The results obtained showed that treatment with oxygen plasma decreased the oxidation potential of the PET surface and revealed the highest affinity for binding of serum components. Accordingly, the cells reflected the best adhesion and morphological properties on oxygen-treated PET polymers. Moreover, treatment with oxygen plasma did not induce intracellular reactive oxygen species production while it stimulated endothelial cell proliferation by 25% suggesting the possible use of oxygen plasma treatment to enhance endothelialization of synthetic vascular grafts.

Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings

“…Oxygen is the most commonly used for plasma treatment of the surface to improve the wettability and controlling of the biocompatibility. It has been shown that the cell proliferation increased by 30% when HEMC-1 cultured on oxygen plasma-treated polymers after 48 h [59]. Oxygen plasma-treated samples could enhance not only the cell adhesion and proliferation, but also the protein adhesion [38].…”

Nanotopography and Plasma Treatment: Redesigning the Surface for Vascular Graft Endothelialisation

“…for endothelialisation). 25 However, use of plasma treatment alone may not be enough, 26 especially as there may be a more subtle role that is played by surface topography. This suggests that a synergistic role may be played by incorporating both treatment approaches, as has been suggested by other authors.…”

Biocompatibility of Plasma-Treated Polymeric Implants

“…This phenomenon was explained by the creation of new functional groups and the modification of surface morphology, which promotes the adhesion of endothelial cells. Numerous studies have proved that plasma treatment significantly improves biocompatible properties of polymer materials [17,45,46,47,48,49,50]. In their study, Jaganjac et al [49] proved that oxygen rich coating after plasma treatment promotes binding of proteins and endothelialization of polyethylene terephthalate polymer.…”

“…Numerous studies have proved that plasma treatment significantly improves biocompatible properties of polymer materials [17,45,46,47,48,49,50]. In their study, Jaganjac et al [49] proved that oxygen rich coating after plasma treatment promotes binding of proteins and endothelialization of polyethylene terephthalate polymer. In another study it was shown that cells prefer to adhere on moderate hydrophobic polymer surfaces, rather than on hydrophilic or super-hydrophilic ones.…”