Mitochondrial 16S rRNA gene-based evolutionary divergence and molecular phylogeny ofBariliusspp.

Abstract

Barilius is an important genus of fish family Cyprinidae, which comprises 22 species from Indian region. This study aimed to characterize five commonly occurring Bariline species, for example, Barilius bakeri. Barilius gatensis. Barilius vagra. Barilius bendelisis and Barilius tileo, across the country using partial mitochondrial 16 S rRNA gene, to estimate the order of inter- and intra-species divergence among these species and to establish phylogenetic and evolutionary relationships. The average inter-specific divergence was estimated as 7.10%. Intra-specific divergence of 0.20% and 0.10% was observed in B. gatensis and B. bendelisis that exhibited three and two haplotypes with 0.70 and 0.60 haplotype diversities, respectively. The NJ and MP phylogenetic trees were constructed using 16 S rRNA sequences along with sequences of the other five Indian cyprinid species retrieved from NCBI. Phylogenetic trees revealed five discrete branches each as a distinct species of the genus and exhibited identical phylogenetic relationship with other cyprinids. The study provided adequate information to distinguish the five Barilius species and indicated the suitability of 16S rRNA gene sequences in genetic divergence and phylogenetic studies.

Phylogenetic Analysis and Genetic Diversity Among Some Red Sea Reef Fishes (Perciformes: Labridae) Based on Partial 16S Mitochondrial Gene Sequences

“…The results of this study underscore the effectiveness of partial 16S mitochondrial gene sequences in elucidating phylogenetic relationships and genetic diversity among the Labridae species. These findings align with previous molecular studies, which have also demonstrated the utility of 16S rDNA as a barcoding system for revealing genetic variation among fish species and validating its suitability for resolving genetic relationships within this fish family (Craig et al, 2001;Pondella et al, 2003;Mitani et al, 2009;Quraishia et al, 2015;Singh et al, 2015;Saad, 2019;Wang, et al, 2023 ;Baldwin et al, 2023).…”

Polymorphic loci analysis of 16s ribosomal RNA gene of economically important marine lizardfish Bombay duck (Harpadon nehereus)

“…Similar type of interspecies polymorphism showed in Barilius spp. (Singh et al 2015) when 16S rRNA gene was compared among Barilius bakeri, B. gatensis, B. vagra, B. bendelisis and B. tile. Phylogenetic analysis: As Bombay duck is a lizardfish, a comparative study was performed among lizardfish from Harpadontidae and synodontidae family using the neighbor joining method, with bootstrap value 1000 where Labeo rohita was used as an out group. In the present study, Harpadon nehereus and H. microchir, belong to the family Harpadontidae, form a monophyletic clade with bootstrap value 96 (Fig.…”

Komposisi Nekleotida Sekuen Gen Mitokondria 16S rRNA Ikan Bilih (Mystacoleucus padangensis Bleeker, 1852) Danau Singkarak, Solok, Sumatera Barat

“…Hal ini disebabkan karena gen mitokondria bersifat maternal sehingga pewarisannya tidak ditemukan adanya rekombinasi seperti halnya gen inti (Hartwell et al, 2015). Gen mitokondria 16S rRNA merupakan gen penanda yang penting dalam karakterisasi dan identifikasi genetik spesies serta untuk mengamati hubungan filogenetik inter spesies (Li et al, 2008;Singh et al, 2013). Adapun penggunaan gen mitokondria 16S rRNA untuk karakterisasi dan identifikasi spesies telah banyak digunakan pada ikan antara lain pada ikan belut (Arisuryanti et al, 2016), ikan Piscirickettsia salmonis dari Chili Selatan (Contreras-Lynch et al, 2015), dan ikan gabus (Firlianty et al, 2014).…”