Polymorphic loci analysis of 16s ribosomal RNA gene of economically important marine lizardfish Bombay duck (Harpadon nehereus)

Saha, Sristy; Ferdous, Zannatul; Jahan, Hawa; Khandaker, Ashfaqul Muid; Shahjahan, Reza Md; Begum, Rowshan Ara

doi:10.3329/bjz.v47i1.42020

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…However, most studies on H. nehereus have been conducted focusing on stock assessment, morphological traits, biological habits and nutrition processing [10][11][12][13], and limited studies involve the field of genetics. For example, these studies include population genetic structure and genetic variation analysis based on simple sequence repeat (SSR), sequence-related amplified polymorphism (SRAP) and mitochondrial DNA (mtDNA) markers [14][15][16][17], the molecular phylogenetic relationship revealed by mtDNA Cyt b, 16S rRNA and the complete mtDNA genome [17][18][19], and the expression analysis of calcium cycling genes by transcriptome sequencing [20]. With the accomplishment of the Human Genome Project (HGP) early in 2001, a new era of genomics has arrived in the field of genetics research.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Survey Reveals the Microsatellite Characteristics and Phylogenetic Relationships of Harpadon nehereus

Yang

Huang

Ning

et al. 2021

CIMB

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Harpadon nehereus forms one of the most important commercial fisheries along the Bay of Bengal and the southeast coast of China. In this study, the genome-wide survey dataset first produced using next-generation sequencing (NGS) was used to provide general information on the genome size, heterozygosity and repeat sequence ratio of H. nehereus. About 68.74 GB of high-quality sequence data were obtained in total and the genome size was estimated to be 1315 Mb with the 17-mer frequency distribution. The sequence repeat ratio and heterozygosity were calculated to be 52.49% and 0.67%, respectively. A total of 1,027,651 microsatellite motifs were identified and dinucleotide repeat was the most dominant simple sequence repeat (SSR) motif with a frequency of 54.35%. As a by-product of whole genome sequencing, the mitochondrial genome is a powerful tool to investigate the evolutionary relationships between H. nehereus and its relatives. The maximum likelihood (ML) phylogenetic tree was constructed according to the concatenated matrix of amino acids translated from the 13 protein-coding genes (PCGs). Monophyly of two species of the genus Harpadon was revealed in the present study and they formed a monophyletic clade with Saurida with a high bootstrap value of 100%. The results would help to push back the frontiers of genomics and open the doors of molecular diversity as well as conservation genetics studies on this species.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Survey Reveals the Microsatellite Characteristics and Phylogenetic Relationships of Harpadon nehereus

Yang

Huang

Ning

et al. 2021

CIMB

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Reveal the Population Genetic Characteristics of Bombay Duck (Harpadon nehereus) in Coastal Waters of China with Genotyping-by-Sequencing Technique

Yang

Huang

Jiang

2022

J. Ocean Univ. China

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Morphogenetic identification of a new record Deudorix livia (Lepidoptera: Lycaenidae) in Assiut Governorate of Northern Upper Egypt

Abdel-Galil,

Mousa,

Abou-Elhagag

et al. 2023

Sci Rep

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Deudorix livia (Klug, 1834) (Lepidoptera: Lycaenidae) is one of the most serious lepidopteran insect pests attacking pomegranate fruit around the world, including Egypt (Assiut Governorate, Upper Egypt). To create an effective program (IPM) to control such harmful pests, accurate identification of the pest morphology and genetic structure is essential. Studies on the morphogenetics of this pest are scarce. So, the goal of this research is to identify it both morphologically and genetically. Pomegranate butterfly immature stages were collected from infested pomegranate fruits and reared in the laboratory until the adult's emergence. By using light and scanning electron microscopy, some morphological structures of males and females were studied. DNA was extracted from the legs of a pomegranate butterfly adult. Also, PCR was conducted by using the mitochondrial CO1 gene for sequencing and phylogenetic tests. The results show that the body scales are a mixture of dark and light gray on the dorsal side and white on the ventral side in both sexes. The average male body length (BL) was 11.674 ± 0.299 mm and was 11.458 ± 1.001 mm for the females. The wing venation is similar in both sexes. For the first time, a partial sequence of the mitochondrial CO1 gene in D. livia was deposited in GenBank (MW463927).

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Polymorphic loci analysis of 16s ribosomal RNA gene of economically important marine lizardfish Bombay duck (Harpadon nehereus)

Cited by 3 publications

References 10 publications

Genome-Wide Survey Reveals the Microsatellite Characteristics and Phylogenetic Relationships of Harpadon nehereus

Genome-Wide Survey Reveals the Microsatellite Characteristics and Phylogenetic Relationships of Harpadon nehereus

Reveal the Population Genetic Characteristics of Bombay Duck (Harpadon nehereus) in Coastal Waters of China with Genotyping-by-Sequencing Technique

Morphogenetic identification of a new record Deudorix livia (Lepidoptera: Lycaenidae) in Assiut Governorate of Northern Upper Egypt

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