Bio-orthogonal and combinatorial approaches for the design of binding growth factors

Ito, Yoshihiro; Tada, Seiichi

doi:10.1016/j.biomaterials.2013.06.037

Cited by 23 publications

(13 citation statements)

References 65 publications

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“…by promoting non-covalent-interaction mediated immobilization or by the addition of enzyme sensitive moieties for highly controlled immobilization) [137]. For example, artificial ECM molecules which are designed to interact with several GFs for their non-covalent immobilization via coiled-coil interactions have been used to fabricate tissue engineering scaffolds and shown to promote angiogenesis [138].…”

Section: In Vitro Techniques For Vascularizationmentioning

confidence: 99%

Microfluidic techniques for development of 3D vascularized tissue

et al. 2014

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Development of a vascularized tissue is one of the key challenges for the successful clinical application of tissue engineered constructs. Despite the significant efforts over the last few decades, establishing a gold standard to develop three dimensional (3D) vascularized tissues has still remained far from reality. Recent advances in the application of microfluidic platforms to the field of tissue engineering have greatly accelerated the progress toward the development of viable vascularized tissue constructs. Numerous techniques have emerged to induce the formation of vascular structure within tissues which can be broadly classified into two distinct categories, namely (1) prevascularization-based techniques and (2) vasculogenesis and angiogenesis-based techniques. This review presents an overview of the recent advancements in the vascularization techniques using both approaches for generating 3D vascular structure on microfluidic platforms.

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Section: In Vitro Techniques For Vascularizationmentioning

confidence: 99%

Microfluidic techniques for development of 3D vascularized tissue

et al. 2014

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“…Recent studies have revealed that the immobilization of growth factor protein enables the stimulation of the mitogenic and differentiating signals from biomaterials. [ 1 , 2 , 3 , 4 , 5 ] Protein tethering has been realized using various methods; however, the immobilization methods for biomacromolecules on inorganic surfaces with maintained bioactivity and molecular orientation are still limited. [ 5 , 6 , 7 , 8 , 9 , 10 , 11 ] Therefore, we aimed to develop an adhesive polypeptide that would form a nanolayer on surfaces and provide mitogenic and differentiating activities.…”

Section: Introductionmentioning

confidence: 99%

Versatile Mitogenic and Differentiation‐Inducible Layer Formation by Underwater Adhesive Polypeptides

et al. 2021

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Artificial materials have no biological functions, but they are important for medical devices such as artificial organs and matrices for regenerative medicine. In this study, mitogenic and differentiation‐inducible materials are devised via the simple coating of polypeptides, which contain the sequence of epidermal growth factor or insulin‐like growth factor with a key amino acid (3,4‐dihydroxyphenylalanine) of underwater adhesive proteins. The adhesive polypeptides prepared via solid‐phase synthesis form layers on various substrates involving organic and inorganic materials to provide biological surfaces. Through the direct activation of cognate receptors on interactive surfaces, the materials enable increased cell growth and differentiation compared to that achieved by soluble growth factors. This superior growth and differentiation are attributed to the long‐lasting signal transduction (triggered by the bound growth factors), which do not cause receptor internalization and subsequent downregulation.

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“…[2][3][4][5][6] However, there are limited procedures for surface modification with biological molecules. To biologically modify metal surfaces, silane-based coupling methods have been conventionally employed to prepare an initial organic layer on the metal surface.…”

Section: Introductionmentioning

confidence: 99%

Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement

Zhou¹,

Park²,

Mao³

et al. 2015

IJN

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Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by 31 P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

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Bio-orthogonal and combinatorial approaches for the design of binding growth factors

Cited by 23 publications

References 65 publications

Microfluidic techniques for development of 3D vascularized tissue

Microfluidic techniques for development of 3D vascularized tissue

Versatile Mitogenic and Differentiation‐Inducible Layer Formation by Underwater Adhesive Polypeptides

Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement

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