Vitrification of blastocysts derived from fair to poor quality cleavage stage embryos can produce high pregnancy rates after warming

Shaw-Jackson, Chloë; Bertrand, Édouard; Becker, B.; Colin, Jérôme; Beaudoin‐Chabot, Caroline; Rozenberg, Serge; Autin, Candice

doi:10.1007/s10815-013-0037-7

Cited by 25 publications

(27 citation statements)

References 43 publications

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“…We have previously demonstrated that embryos that do not fulfill the current morphological or morphokinetic criteria still have the potential to develop to a blastocyst and lead to the birth of a healthy baby [48]. Such observations are supported by other studies showing that even vitrification of blastocysts derived from fair-to poor-quality cleavage-stage embryos can produce high pregnancy rates after warming [49,50].…”

Section: Discussionmentioning

confidence: 55%

The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging—a retrospective observational study

Neyer

Zintz

Stecher

et al. 2015

J Assist Reprod Genet

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Purpose Various time-lapse studies have postulated embryo selection criteria based on early morphokinetic markers. However, late paternal effects are mostly not visible before embryonic genome activation. The primary objective of this retrospective study was to investigate whether those early morphokinetic algorithms investigated by time-lapse imaging are reliable enough to allow for the accurate selection of those embryos that develop into blastocysts, while of course taking into account the correlation with the type of injected spermatozoa. Methods During a period of 18 months, a total of 461 MII oocytes from 43 couples with severe male factor infertility and previous Bexternal^IVF failures after cleavage-stage embryo transfer (ET) were fertilized by intracytoplasmic morphologically selected sperm injection (IMSI). Thereof, 373 embryos were monitored in a time-lapse incubator until ET on day 5. Blastocyst outcome in combination with three previously postulated MKc (cc2: t3-t2, 5-12 h; t3, 35-40 h; t5, 48-56 h) and the morphology of the selected sperm were analyzed. Results A significant increase in the rate of blastocysts (54.0 vs. 36.3 %; P<0.01) and top blastocysts (25.3 vs. 10.8 %; P<0.001) was observed in the group of those meeting all three morphokinetic criteria (MKc3). However, MKc3 were only met in 23.3 % of all embryos. Moreover, TBR was influenced by the type of injected spermatozoa. In both groups, TBR decreased dramatically (MKc3, 35.0 vs. 17.0 %; MKc<3, 14.2 vs. 8.4 %) when class II/III sperm instead of class I were injected. Conclusion Early morphokinetic parameters might give some predictive information but fail to serve as a feasible selective tool for the prediction of blastocyst development given the influence of the type of spermatozoa injected.

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Section: Discussionmentioning

confidence: 55%

The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging—a retrospective observational study

Neyer

Zintz

Stecher

et al. 2015

J Assist Reprod Genet

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“…The embryos were evaluated and fixed immediately, or cultured another 24 hr (Days‐4 and ‐6 embryos) or 48 hr (Day‐7 embryos) before evaluation and fixation. A few of the poor‐quality embryos that became available at Day 3 developed into good‐quality embryos, which was reported before (Ren et al, ; Shaw‐Jackson et al, ); however, the majority remained of poor‐quality, so those embryos were included in the poor‐quality group.…”

Section: Methodsmentioning

confidence: 59%

Similar kinetics for 5‐methylcytosine and 5‐hydroxymethylcytosine during human preimplantation development in vitro

Petrussa

Rycke

2016

Molecular Reproduction Devel

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After fertilization, the mammalian embryo undergoes epigenetic reprogramming with genome-wide DNA demethylation and subsequent remethylation. Oxidation of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) was suggested to be an intermediate step in the DNA demethylation pathway. Other evidence, such as the stability of 5hmC in specific tissues, suggests that 5hmC constitutes a new epigenetic modification with its own biological function. Since few studies have been conducted on human material compared to animal models and species-specific epigenetic differences have been reported, we studied global DNA methylation and hydroxymethylation patterns in human in vitro preimplantation embryos using immunocytochemistry, comparing these patterns in good-quality and abnormally developing embryos. Our data showed that DNA methylation and hydroxymethylation modifications co-exist. 5mC and 5hmC signals were found in oocytes and in paternal and maternal pronuclei of zygotes, present in non-reciprocal patterns-which contrasts published data for the mouse. These two epigenetic modifications are present between Days 1 and 7 of in vitro development, with 5mC levels declining over cell divisions without noticeable remethylation during this period. A main decline in 5mC and 5hmC occurred as the embryo progressed from compaction to the blastocyst stage. No difference in (hydroxy)methylation was found between the inner cell mass and trophectoderm. When comparing normally and abnormally developing embryos, DNA (hydroxy)methylation reprogramming was abnormal in poor-quality embryos, especially during the first cleavages. Mol. Reprod. Dev. 83: 594-605, 2016 © 2016 Wiley Periodicals, Inc.

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“…Although previous studies have already reported a potential for PQE to develop to the blastocyst stage, with some even capable of implantation (Alikani et al, 2000;Balaban et al, 2001;Shaw-Jackson et al, 2013;Stone et al, 2005), these embryos are usually destroyed or destined for research purposes. A possible explanation may be the poor morphology of these embryos on day 3, which is not compatible with freezing programmes.…”

Section: Discussionmentioning

confidence: 99%

Is it acceptable to destroy or include human embryos before day 5 in research programmes?

Poulain

Hesters

Sanglier

et al. 2014

Reproductive BioMedicine Online

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Vitrification of blastocysts derived from fair to poor quality cleavage stage embryos can produce high pregnancy rates after warming

Abstract: Supernumerary average quality day 3 embryos should be given a second chance to be selected for cryopreservation. If blastocysts are obtained and survive vitrification, there is a good chance of implantation thus reducing embryo waste.

Cited by 25 publications

References 43 publications

The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging—a retrospective observational study

The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging—a retrospective observational study

Similar kinetics for 5‐methylcytosine and 5‐hydroxymethylcytosine during human preimplantation development in vitro

Is it acceptable to destroy or include human embryos before day 5 in research programmes?

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