Is it acceptable to destroy or include human embryos before day 5 in research programmes?

Poulain, Marine; Hesters, Laëtitia; Sanglier, Thibaut; Bantel, Astrid de; Fanchin, Rénato; Frydman, Nelly; Grynberg, Michaël

doi:10.1016/j.rbmo.2013.12.007

Cited by 13 publications

(17 citation statements)

References 47 publications

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“…Our results are better than the two previously mentioned studies. Poulain et al reported a blastulation rate of 35% of which 33.6% were cryopreserved [7] and Kaartinen et al found that 19.7% of PQE were frozen after extended culture [2]. As embryo grading was based on the ESHRE consensus in Poulain's study and in ours, a possible explanation of the higher blastulation rate in our study could be the optimization of culture conditions in our laboratory.…”

Section: Discussionsupporting

confidence: 43%

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Extended culture of poor-quality supernumerary embryos improves ART outcomes

Sallem

Santulli

Barraud-Lange

et al. 2017

J Assist Reprod Genet

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Section: Discussionsupporting

confidence: 43%

“…Recently, some reports have shown that the practice of culturing poor-quality day-2 embryos could positively impact ART outcomes [2,7]. They, however, reported smaller numbers of cultured embryos.…”

Section: Discussionmentioning

confidence: 99%

Extended culture of poor-quality supernumerary embryos improves ART outcomes

Sallem

Santulli

Barraud-Lange

et al. 2017

J Assist Reprod Genet

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“…However, they did not display the outcomes of transferring blastocysts originating from good-quality cleavage stage embryos. Poulain et al (16) carried out a prospective study including 33 blastocysts transfer cycles. Thirty-four blastocysts originating from PQE yielded ten clinical pregnancies and five live births.…”

Section: Pregnancy Outcomes Of the Blastocysts Originating From Poor-mentioning

confidence: 99%

Miscarriage Rate Is High With Frozen-Thawed Blastocysts Arising From Poor-Quality Cleavage Stage Embryos

Xia

Zhao

et al. 2020

Front. Endocrinol.

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Embryos with low morphological scores can still develop to the blastocyst stage and result in good clinical outcomes. However, no studies have reported the possible effects of transferring cryopreserved blastocysts developed from poor-quality cleavage stage embryos on pregnancy and perinatal outcomes. In this retrospective study, the clinical value of transferring blastocysts derived from day 3 poor-quality cleavage stage embryos during in vitro fertilization and embryo transfer procedures was evaluated. According to the quality of embryos on day 3 from which the transferred blastocyst originated, patients were divided into three groups: poor-quality (111 cycles, group A), good-quality (235 cycles, group B), and top-quality (119 cycles, group C). Group A experienced the highest miscarriage rate (30.2%) which was increased when compared to group C (12.5%) (P = 0.03). The clinical pregnancy rates and live birth rates were not significantly different among the three groups. However, good blastocyst originating from top day 3 embryos resulted in higher live birth rate. Of the 218 live births, no differences in obstetric and perinatal outcomes were noted among the three groups. The results showed that extended culture of poor-quality cleavage stage embryos could resulted in favorable clinical pregnancy rates but at a higher incidence of miscarriages. Meanwhile, the risk of adverse perinatal outcomes was not increased.

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“…Therefore, each clinic makes its own decisions about the fate of poor-quality embryos. These could be used for stem cell establishment (Reubinoff et al, 2000), but most are likely to be discarded or used for research (Poulain et al, 2014). An alternative practice is to continue culture out to the blastocyst stage to confirm whether blastocysts development occurs.…”

Section: Articlementioning

confidence: 99%

“…Based on the documented associations between embryo quality and a number of IVF performance metrics (Giorgetti et al, 1995;Grillo et al, 1991;Oron et al, 2014;Scott et al, 2007;Shulman et al, 1993;Van Royen et al, 1999;Zhu et al, 2014a;Ziebe et al, 1997), poor quality embryos based on morphologic criteria are often not used during ART (Bradley et al, 2014); eventual uses include disposal, culture to blastocyst stage then embryo transfer, and use in research programs (Kaartinen et al, 2015;Poulain et al, 2014;Wang et al, 2012). It is important to note, however, that morphologic examination of embryos may not be sufficient to reliably assess their developmental potential (Meseguer et al, 2012) which is also confirmed by the low reproducibility of grading of the morphological characteristics of embryos varies by experienced embryologists (Storr et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Live Birth Rate After Transfer of Fresh or Frozen Poor Quality Day-3 Embryos Only

Vuong

Pham

Huynh

et al. 2019

FandR

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Background: Embryo quality is an important predictor of successful outcome in in vitro fertilization (IVF). However, current knowledge on the live birth rate after transfer of poor quality embryos is limited. This study investigated the live birth rate after transfer of only poor quality day-3 embryos in women undergoing IVF. Methods: This retrospective study included 153 couples who underwent IVF at IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam between June 2014 and January 2017 and had only poor quality day-3 embryos available for fresh (n [Formula: see text] 102) or frozen (n [Formula: see text] 51) transfer. The control group included patients who had transfer of one good embryo (n [Formula: see text] 64). Embryos were rated using the Istanbul criteria. Results: In the poor quality embryo group, the mean number of oocytes retrieved and number of embryos were 7.5 ± 4.4 and 1.8 ± 0.9, respectively. Mean number of embryos transferred was 1.6 ± 0.5 in the fresh transfer group and 2.0 ± 0.2 in the freeze-only group. Live births did occur after transfer of poor quality embryos, but the implantation, clinical pregnancy and live birth rates were significantly lower than after fresh or frozen transfer of a single good quality embryo (9.5 vs. 26.6%, p < 0.001; 13.7 vs. 26.6%, p < 0.001; and 7.2 vs. 18.8%, p [Formula: see text] 0.02, respectively). Conclusions: Live birth was achieved after transfer of only poor quality embryos in women undergoing IVF. This suggests that transfer of poor quality embryos could be an option when higher grade embryos are not available, after the chances of live birth have been discussed with the patient.

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Is it acceptable to destroy or include human embryos before day 5 in research programmes?

Cited by 13 publications

References 47 publications

Extended culture of poor-quality supernumerary embryos improves ART outcomes

Extended culture of poor-quality supernumerary embryos improves ART outcomes

Miscarriage Rate Is High With Frozen-Thawed Blastocysts Arising From Poor-Quality Cleavage Stage Embryos

Live Birth Rate After Transfer of Fresh or Frozen Poor Quality Day-3 Embryos Only

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