Cycling probe technology to quantify and discriminate between wild-type varicella-zoster virus and Oka vaccine strains

Ihira, Masaru; Higashimoto, Yuki; Kawamura, Yoshiki; Sugata, Ken; Ohashi, Masahiro; Asano, Yoshizo; Yoshikawa, Tetsushi

doi:10.1016/j.jviromet.2013.06.031

Cited by 11 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Commercial kits combining quantitative real-time PCR with CPTbased detection have been used for rapid detection and quantification of HHV-6 and can also discriminate between HHV-6A and HHV-6B [109]. CPT has also been employed for differentiating strains of VZV [110], rapid and specific detection of resistant influenza A viruses [111], and HCV [112].…”

Section: Cycling Probe Technology (Cpt)mentioning

confidence: 99%

Amplification chemistries in clinical virology

Dunbar

Das

2019

Journal of Clinical Virology

View full text Add to dashboard Cite

Molecular diagnostic methods have evolved and matured considerably over the last several decades and are constantly being evaluated and adopted by clinical laboratories for the identification of infectious pathogens. Advancement in other technologies such as fluorescence, electronics, instrumentation, automation, and sensors have made the overall diagnostic process more accurate, sensitive, and rapid. Nucleic acid based detection procedures, which rely on the fundamental principles of DNA replication have emerged as a popular and standard diagnostic method, and several commercial assays are currently available based on different nucleic acid amplification techniques. This review focuses on the major amplification chemistries that are used for developing commercial assays and discusses their application in the clinical virology laboratory. Polymerase chain reaction (PCR)The development of PCR by Kary Mullis in the 1980s revolutionized the detection of microbial pathogens in clinical specimens. PCR is a highly sensitive method that involves cyclical heating and cooling of the reaction mixture to facilitate amplification of the target DNA [3]. The method consists of three basic steps (denaturation, annealing, and extension) and the reaction mixture requires four primary components (template DNA, primers, nucleotides, and a DNA polymerase) (Fig. 1a). Double-stranded target DNA (dsDNA) is thermally denatured to form a single-stranded DNA template (ssDNA). Oligonucleotide primers anneal to complementary target sequences on the nucleic acid template and are extended by the DNA polymerase, thus creating the resultant PCR product (amplicon). The reaction occurs in a programmable thermal https://doi.

show abstract

Section: Cycling Probe Technology (Cpt)mentioning

confidence: 99%

Amplification chemistries in clinical virology

Dunbar

Das

2019

Journal of Clinical Virology

View full text Add to dashboard Cite

show abstract

“…They are more sensitive than viral cultures, DFA and Tzanck smears particularly when primers for genes 28 and 29 are used [30,428,429,440,449,450,457] . Molecular biology tests are also useful in the case of varicella appearing 7-42 d after vaccination, in the case of herpes zoster appearing 42 d after vaccination and, in the case of the suspected transmission of the vaccine virus, can distinguish virus vaccine, wild-type virus and potential recombinants of vaccine and wild-type viruses [30,451,452,454,455,[458][459][460][461][462][463] , although some tests have proved to be less appropriate over time for these purposes [30] . There are also some multiplex assays that can differentiate HSV-1, HSV-2 and VZV in cutaneous and mucocutaneous samples, making them very useful in the case of skin lesions of unclear etiology or immunosuppressed patients [429,464,465] .…”

Section: Direct Fluorescent Antibody Assaymentioning

confidence: 99%

Microbiology laboratory and the management of mother-child varicella-zoster virus infection

Paschale

Clerici

2016

WJV

View full text Add to dashboard Cite

Varicella-zoster virus, which is responsible for varicella (chickenpox) and herpes zoster (shingles), is ubiquitous and causes an acute infection among children, especially those aged less than six years. As 90% of adults have had varicella in childhood, it is unusual to encounter an infected pregnant woman but, if the disease does appear, it can lead to complications for both the mother and fetus or newborn. The major maternal complications include pneumonia, which can lead to death if not treated. If the virus passes to the fetus, congenital varicella syndrome, neonatal varicella (particularly serious if maternal rash appears in the days immediately before or after childbirth) or herpes zoster in the early years of life may occur depending on the time of infection. A Microbiology laboratory can help in the diagnosis and management of mother-child infection at four main times: (1) when a pregnant woman has been exposed to varicella or herpes zoster, a prompt search for specific antibodies can determine whether she is susceptible to, or protected against infection; (2) when a pregnant woman develops clinical symptoms consistent with varicella, the diagnosis is usually clinical, but a laboratory can be crucial if the symptoms are doubtful or otherwise unclear (atypical patterns in immunocompromised subjects, patients with post-vaccination varicella, or subjects who have received immunoglobulins), or if there is a need for a differential diagnosis between varicella and other types of dermatoses with vesicle formation; (3) when a prenatal diagnosis of uterine infection is required in order to detect cases of congenital varicella syndrome after the onset of varicella in the mother; and (4) when the baby is born and it is necessary to confirm a diagnosis of varicella (and its complications), make a differential diagnosis between varicella and other diseases with similar symptoms, or confirm a causal relationship between maternal varicella and malformations in a newborn. Core tip: Although varicella during pregnancy is infrequent and congenital varicella syndrome (CVS) is rare, every available means should be used to prevent and diagnose them. Microbiology laboratories can be crucial in these situations: Evaluating a mother's immune status with sensitive and specific tests for the detection of antibodies; allowing a rapid diagnosis with molecular biology tests when a clinical manifestation may be due to different etiologies; following pregnant women with varicella for the prenatal diagnosis of CVS with close collaboration between molecular biology investigators and specialists in imaging diagnostics.De Paschale M, Clerici P. Microbiology laboratory and the management of mother-child varicella-zoster virus infection. World

show abstract

“…Human herpesvirus 6A ve B saptanması için geliştirilen CP temelli real-time PCR testi bunun bir örneğidir (23). CP real-time PCR tekniği vahşi-tip varicella-zoster virus (VZV) ve Oka aşı kökenlerinin birbirinden ayırt edilmesi ve kantitasyonu için de kullanılmıştır (24). CP real-time PCR tekniği ile dizi analizi yönteminin kombine edildiği bir çalışmada ise NS5A inhibitörlerine dirençli HCV varyantlarının saptanması ve kantitasyonu başarılı bir şekilde test edilmiştir (25).…”

Section: Introductionunclassified

Si̇nyal Ampli̇fi̇kasyon Tekni̇kleri̇ Ve Tanisal Vi̇roloji̇deki̇ Uygulamalari

Şahiner¹,

Gümral²

2020

J Istanb Fac Med

View full text Add to dashboard Cite

Cycling probe technology to quantify and discriminate between wild-type varicella-zoster virus and Oka vaccine strains

Cited by 11 publications

References 28 publications

Amplification chemistries in clinical virology

Amplification chemistries in clinical virology

Microbiology laboratory and the management of mother-child varicella-zoster virus infection

Si̇nyal Ampli̇fi̇kasyon Tekni̇kleri̇ Ve Tanisal Vi̇roloji̇deki̇ Uygulamalari

Contact Info

Product

Resources

About