Hedgehog in the Drosophila testis niche: what does it do there?

Zhang, Zhao; Pan, Chenyu; Zhao, Yun

doi:10.1007/s13238-013-3040-y

Cited by 14 publications

(14 citation statements)

References 56 publications

(13 reference statements)

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“…GSC homeostasis is dependent upon cross-talk of signalling pathways between somatic CySCs and hub cells with GSCs (reviewed in de Cuevas & Matunis 2011, Greenspan et al 2015. Janus Kinase-Signal Transducer and Activator of Transcription (JAK-STAT) signalling (Kiger et al 2001, Tulina & Matunis 2001, Leatherman & Dinardo 2010; the bone morphogenesis protein (BMP) pathway (Kawase et al 2004, Michel et al 2011, Inaba et al 2015 and hedgehog signalling (Michel et al 2012, Amoyel et al 2013, Zhang et al 2013) are all involved in regulating early germ cell development, with short-range signals being received in the GSCs from either (or both) the hub cells or CyCSs.…”

Section: Tools To Study the Drosophila Testis Niche: An Imaging Approachmentioning

confidence: 99%

“…Somatic hub cells (red) secrete the ligand unpaired (Upd), which in turn activate Janus Kinase-Signal Transducer and Activator of Transcription (JAK-STAT) signalling in adjacent GSCs (light blue) and CySCs (yellow) (Leatherman & DiNardo 2008, Flaherty et al 2010. Hedgehog (HH) signals are also received by the CySCs from the hub cells (Amoyel et al 2013, Zhang et al 2013. The ligand Decapentaplegic (Dpp) is also secreted from the hub cells to activate Bone morphogenetic protein (BMP) signalling in adjacent GSCs (Inaba et al 2015) GSCs divide to self-renew and produce a daughter gonialblast (blue).…”

Section: Tools To Study the Drosophila Testis Niche: An Imaging Approachmentioning

confidence: 99%

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A Drosophila toolkit for defining gene function in spermatogenesis

Siddall¹,

Hime²

2017

Reproduction

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Expression profiling and genomic sequencing methods enable the accumulation of vast quantities of data that relate to the expression of genes during the maturation of male germ cells from primordial germ cells to spermatozoa and potential mutations that underlie male infertility. However, the determination of gene function in specific aspects of spermatogenesis or linking abnormal gene function with infertility remain rate limiting, as even in an era of CRISPR analysis of gene function in mammalian models, this still requires considerable resources and time. Comparative developmental biology studies have shown the remarkable conservation of spermatogenic developmental processes from insects to vertebrates and provide an avenue of rapid assessment of gene function to inform the potential roles of specific genes in rodent and human spermatogenesis. The vinegar fly, Drosophila melanogaster, has been used as a model organism for developmental genetic studies for over one hundred years, and research with this organism produced seminal findings such as the association of genes with chromosomes, the chromosomal basis for sexual identity, the mutagenic properties of X-irradiation and the isolation of the first tumour suppressor mutations. Drosophila researchers have developed an impressive array of sophisticated genetic techniques for analysis of gene function and genetic interactions. This review focuses on how these techniques can be utilised to study spermatogenesis in an organism with a generation time of 9 days and the capacity to introduce multiple mutant alleles into an individual organism in a relatively short time frame.Reproduction (2017) 153 R121-R132

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Section: Tools To Study the Drosophila Testis Niche: An Imaging Approachmentioning

confidence: 99%

Section: Tools To Study the Drosophila Testis Niche: An Imaging Approachmentioning

confidence: 99%

A Drosophila toolkit for defining gene function in spermatogenesis

Siddall¹,

Hime²

2017

Reproduction

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“…When the Hh signaling pathway is overactivated in CySCs, the number of SSCs sharply decreases probably because of the competition of abnormal proliferous CySCs. However, when the Hh signaling pathway is insufficiently activated, the number of CySCs decreases, and they fail to form the proper BMP concentration in the niche, which negatively affects the regeneration of SSCs [ 26 ]. Hence, controlling Hh signaling accurately in the SSC niche helps maintain SSCs, and it likely benefits the Drosophila spermatogenic cycle.…”

Section: Effect Of the Hedgehog Signaling Pathway On Sscsmentioning

confidence: 99%

Role of the Hedgehog Signaling Pathway in Regulating the Behavior of Germline Stem Cells

Wang

Chen

et al. 2017

Stem Cells International

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Germline stem cells (GSCs) are adult stem cells that are responsible for the production of gametes and include spermatogonial stem cells (SSCs) and ovarian germline stem cells (OGSCs). GSCs are located in a specialized microenvironment in the gonads called the niche. Many recent studies have demonstrated that multiple signals in the niche jointly regulate the proliferation and differentiation of GSCs, which is of significance for reproductive function. Previous studies have demonstrated that the hedgehog (Hh) signaling pathway participates in the proliferation and differentiation of various stem cells, including GSCs in Drosophila and male mammals. Furthermore, the discovery of mammalian OGSCs challenged the traditional opinion that the number of primary follicles is fixed in postnatal mammals, which is of significance for the reproductive ability of female mammals and the treatment of diseases related to germ cells. Meanwhile, it still remains to be determined whether the Hh signaling pathway participates in the regulation of the behavior of OGSCs. Herein, we review the current research on the role of the Hh signaling pathway in mediating the behavior of GSCs. In addition, some suggestions for future research are proposed.

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“…Interestingly, very recent findings revealed that the Hedgehog (Hh) ligand secreted from the hub cells activates the Hh signaling in CySCs (and not in the GSCs) with critical function in CySC maintenance [62][63][64][65]. Hh overexpression leads in increased number of CySCs, identified as Zfh-1 positive cyst cells outside the niche, which can still proliferate in contrast to the normal post-mitotic SCCs.…”

Section: Signaling Regulation Of Stemness Versus Differentiationmentioning

confidence: 99%