Interaction of acriflavine with pyrimidines: A spectroscopic approach

Manivannan, C.; Sambathkumar, S.; Renganathan, R.

doi:10.1016/j.saa.2013.05.034

Cited by 14 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, presence of substituent in catechol molecule in uences the release of H • from aromatic hydroxyl groups (-OH). Fluorescence quenching of 9-aminoacridine and acri avine by various antioxidants such as estrogens, avonoids, phenol and its derivatives, catechols and uracils were thoroughly investigated and found to undergo pronounced charge transfer process [16][17][18][19][20][21]. Recently, NB employed as a uorescent probe to evaluate the antioxidants of uracil molecules by abstracting the release of H • from antioxidants via ground state complex formation [22].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic Study on the Reaction of Singlet-Excited Nile Blue with Certain Antioxidants

Manivannan¹,

Baskaran²,

Anbazhagan³

2021

Preprint

Self Cite

View full text Add to dashboard Cite

The photoinduced interaction of nile blue (NB) with various antioxidant molecules was investigated by fluorescence quenching technique and lifetime measurements. The various substituted catecholic compounds are employed as quenchers to evaluate their antioxidant activity. The formations of ground state complex between NB and quencher molecules observed from the UV-Visible absorption spectroscopy. The bimolecular quenching rate constants (kq) values depend on presence of substituent and its electronic properties of quencher molecules. Fluorescence quenching experiments have been performed at three different temperatures to assess the thermodynamic parameters. Time resolved fluorescence measurements suggest that the fluorescence quenching of NB with antioxidant molecules undergoes static quenching mechanism. The bond dissociation enthalpy (BDE) values reveal the discharge of HTfrom the antioxidant molecules. The electronic properties play an important role in the antioxidant activity of quencher molecules. The mechanism of fluorescence quenching between NB and quencher molecules are analysed based on the fluorescence quenching experiments, cyclic voltammetry experiments and BDE calculations.

show abstract

Section: Introductionmentioning

confidence: 99%

Spectroscopic Study on the Reaction of Singlet-Excited Nile Blue with Certain Antioxidants

Manivannan¹,

Baskaran²,

Anbazhagan³

2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the second decade of the 20th century, the first synthesis of acriflavine in the form of an acridine derivative was achieved . There are various applications for acriflavine such as antibacterial drugs, anticancer, an AIDS curative, as well as a fluorescent and absorptive reagent for identifying nucleic acid structures and in preparation of dyes …”

Section: Introductionmentioning

confidence: 99%

Sensitive spectrofluorometric method for the determination of ascorbic acid in pharmaceutical nutritional supplements using acriflavine as a fluorescence reagent

et al. 2019

View full text Add to dashboard Cite

An easily performed, specific, sensitive, rapid, reliable and inexpensive procedure for the spectrofluorometric quantitation of ascorbic acid was proposed using acriflavine as a fluorescence quenching reagent. The procedure was based on the determined quenching effect of ascorbic acid on the natural fluorescence signal of acriflavine and the reaction between ascorbic acid and acriflavine in Britton-Robinson buffer solution (pH 6) to produce an ion-associated complex. The reduction in acriflavine fluorescence intensity was detected at 505 nm, while excitation occurred at 265 nm. The relationship between quenching fluorescence intensity (ΔF) and concentration of ascorbic acid was linear (R 2 = 0.9967) within the range 2-10 μg/ml and with a detection limit of 0.08 μg/ml. No significant interference was detected from other materials often found in pharmaceutical nutritional tablets. The obtained results were compared with those from high-performance liquid chromatography and appeared in good agreement, with no important differences in precision or accuracy. The proposed spectrofluorimetric method was used to determine the amount of ascorbic acid in a number of commercial pharmaceutical nutritional supplement tablets with a 95% confidence performance.

show abstract

“…Recently, it has been discovered to have anticancer activity and used in AIDS treatments . It was commonly identified as an efficient laser dye due to its excellent spectroscopic and pharmacological properties …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Acriflavine is a member of the acredine derivatives, which was first synthesized in 1912 and was used as an absorptive and fluorescent probe for detecting nucleic acid structures. [28,29] Recently, it has been discovered to have anticancer activity and used in AIDS treatments. [30] It was commonly identified as an efficient laser dye due to its excellent spectroscopic and pharmacological properties.…”

mentioning

confidence: 99%

See 1 more Smart Citation

A sensitive fluorescence quenching method for the detection of tartrazine with acriflavine in soft drinks

et al. 2017

View full text Add to dashboard Cite

In this work, a simple, rapid, sensitive, selective spectrofluorimetric method was applied to detect tartrazine. The fluorescence of acriflavine could be efficiently quenched by tartrazine. The method manifested real time response as well as presented satisfied linear relationship to tartrazine. The linear response range of tartrazine (R = 0.9995) was from 0.056 to 5 μmol L . The detection limit (3σ/k) was 0.017 μmol L , indicating that this method could be applied to detect traces of tartrazine. The accuracy and precision of the method was further assured by recovery studies via a standard addition method, with percentage recoveries in the range of 96.0% to 103.0%. Moreover, a quenching mechanism was investigated systematically by the linear plots at varying temperatures based on the Stern-Volmer equation, fluorescence lifetime and UV-visible absorption spectra, all of which proved to be static quenching. This sensitive, selective assay possessed a great application prospect for the food industry owing to its simplicity and rapidity for the detection of tartrazine.

show abstract

Interaction of acriflavine with pyrimidines: A spectroscopic approach

Cited by 14 publications

References 33 publications

Spectroscopic Study on the Reaction of Singlet-Excited Nile Blue with Certain Antioxidants

Spectroscopic Study on the Reaction of Singlet-Excited Nile Blue with Certain Antioxidants

Sensitive spectrofluorometric method for the determination of ascorbic acid in pharmaceutical nutritional supplements using acriflavine as a fluorescence reagent

A sensitive fluorescence quenching method for the detection of tartrazine with acriflavine in soft drinks

Contact Info

Product

Resources

About