Sensitive spectrofluorometric method for the determination of ascorbic acid in pharmaceutical nutritional supplements using acriflavine as a fluorescence reagent

Ali, Layth I. Abd; Qader, Aryan F.; Salih, Musher Ismael; Aboul‐Enein, Hassan Y.

doi:10.1002/bio.3589

Cited by 24 publications

(13 citation statements)

References 47 publications

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“…The low values of LOD indicated high sensitivity. [ 17 ] Comparison of the proposed method with various previously reported methods demonstrated its superiority (Table S2).…”

Section: Resultsmentioning

confidence: 86%

Spectrofluorometric method for the determination of ascorbic acid in pharmaceutical preparation using l‐tyrosine as fluorescence probe

Chen

Zhao

et al. 2020

Luminescence

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Ascorbic acid is a vital nutrient and antioxidant that is commonly used as an additive in commercial products. Quantitation of ascorbic acid is highly desired in the medical, food, and cosmetic industries. A spectrofluorometric assay for sensitive determination of ascorbic acid was developed using L-tyrosine as a fluorescent probe. The native fluorescence intensity of tyrosine was quenched using ascorbic acid. The linear range was 0.03-30.00 μM, and the limit of detection was 0.01 μM. The method exhibited excellent precision, accuracy, specificity, and robustness. Components of pharmaceutical preparations that are commonly found with ascorbic acid did not interfere with detection. The procedure was successfully employed for determination of ascorbic acid content in pharmaceutical tablets, injections, and nutrient supplements with satisfactory results. A Stern-Volmer plot and fluorescence lifetime revealed that quenching was attributed to the inner filter effect and static quenching. Isothermal titration calorimetry confirmed the formation of a complex between tyrosine and ascorbic acid, with a binding constant of 1.68 × 10 3 M −1 and reaction stoichiometry of 0.94. Thermodynamic parameters suggested spontaneous complexation via hydrophobic interactions as the dominant binding force. This method is promising for the simple and rapid determination of ascorbic in the pharmaceutical industry.

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“…The low values of LOD indicated high sensitivity. [ 17 ] Comparison of the proposed method with various previously reported methods demonstrated its superiority (Table S2).…”

Section: Resultsmentioning

confidence: 86%

Spectrofluorometric method for the determination of ascorbic acid in pharmaceutical preparation using l‐tyrosine as fluorescence probe

Chen

Zhao

et al. 2020

Luminescence

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“…Acridine dyes like acriflavine are highly fluorescent natural compounds that can be used as derivatizing agents [ 34 ]. Acriflavine was used for estimation of compounds having pharmaceutical interest such as ascorbic acid [ 35 ]. CFZ is non-fluorescent and has a free carboxylic group that ionizes (forming an anion) under alkaline conditions (pH 8).…”

Section: Resultsmentioning

confidence: 99%

Green and sensitive spectrofluorimetric method for the determination of two cephalosporins in dosage forms

et al. 2021

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Using two green and sensitive spectrofluorimetric methods, we quantified two cephalosporins, cefepime (CFM) and cefazolin (CFZ), in raw and pharmaceutical formulations. The first method is based on the reaction between CFM and fluorescamine (borate buffer, pH 8), which yields a highly fluorescent product. After excitation at 384 nm, the fluorescent product emits light at 484 nm. At concentration ranges from 12.0 to 120.0 ng ml −1 , the relative fluorescence intensity/concentration curve was linear with a limit of quantification (LOQ) of 2.46 ng ml −1 . The second method relied on measuring the CFZ quenching action on acriflavine fluorescence through formation of an ion-associate complex using Britton–Robinson buffer at pH 8. We measured acriflavine fluorescence at 505 nm after excitation at 265 nm. The decrease in acriflavine fluorescence intensity was CFZ concentration-dependent. Using this method, we quantified CFZ in concentrations ranging from 1 to 10 µg ml −1 with a LOQ of 0.48 µg ml −1 . We studied and optimized the factors influencing reaction product formation. Moreover, we adapted our methods to the investigation of the mentioned drugs in raw and pharmaceutical formulations with greatly satisfying results. We statistically validated our methods according to International Council on Harmonisation Guidelines. The obtained results were consistent with those obtained with the official high-performance liquid chromatography methods.

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“…Solution pH is considered a critical parameter of the medium and is required to permit protonation of acriflavine and the reaction to proceed [8,9]. BRB has been investigated over the pH range of 3-10, and it was found that the optimum pH was 7 for both OLS and SUL (figure 3a).…”

Section: Buffer Ph and Volumementioning

confidence: 99%

“…At the specified pH, the positively charged nitrogen atom in acriflavine reacts with the carboxylate anion of OLS or SUL. Hence, electrostatic forces contribute to ion-pair complex formation, as illustrated in scheme 1 [8,9].…”

Section: Determination Of Quenching Mechanism and Stoichiometry Of The Reactionmentioning

confidence: 99%

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Studying the quenching resulted from the formation of an association complex between olsalazine or sulfasalazine with acriflavine

Tolba

Elmansi

2021

R. Soc. open sci.

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We report the detection and quantification of important ulcerative colitis drugs olsalazine (OLS) and sulfasalazine (SUL) by the spectrofluorometric method. The proposed method was optimized and validated by using the quenching effect on the acriflavine fluorescence. The method was applied on the detection and quantification of OLS and SUL under optimized conditions showing the calibration curves were linear (range: 1.0–10.0 µg ml −1 ), with correlation coefficients R 2 of 0.9999 for both drugs. The limits of detection (LOD) and quantification (LOQ) were 53 and 104 ng ml −1 for the OLS and 160 and 315 ng ml −1 for the SUL. This method permitted the analysis of OLS and SUL in their pure and pharmaceutical forms. The proposed spectrofluorimetric method was also evaluated against ‘green’ criteria and all the experimental results make it an eco-friendly and safe method for the detection of OLS and SUL.

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Sensitive spectrofluorometric method for the determination of ascorbic acid in pharmaceutical nutritional supplements using acriflavine as a fluorescence reagent

Cited by 24 publications

References 47 publications

Spectrofluorometric method for the determination of ascorbic acid in pharmaceutical preparation using l‐tyrosine as fluorescence probe

Spectrofluorometric method for the determination of ascorbic acid in pharmaceutical preparation using l‐tyrosine as fluorescence probe

Green and sensitive spectrofluorimetric method for the determination of two cephalosporins in dosage forms

Studying the quenching resulted from the formation of an association complex between olsalazine or sulfasalazine with acriflavine

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