UHPLC-MS Simultaneous Determination and Pharmacokinetic Study of Three Aromatic Acids and One Monoterpene in Rat Plasma after Oral Administration of Shaofu Zhuyu Decoction

Abstract

We developed a sensitive and rapid method for determination of ferulic acid, caffeic acid, vanillic acid, and paeoniflorin in rat plasma based on ultra high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The separation of the four compounds was carried out on an AcQuity UHPLC™ BEH C18 column using a mobile phase consisting of acetonitrile and water (containing 0.1% formic acid). Electrospray ionization in positive and negative ion mode and multiple reaction monitoring was used to identify and quantify active components. All calibration curves gave good linearity (r > 0.991) over the concentration range from 4.24-2875 ngmL(-1) for all components. The precision of the in vivo study was evaluated by intraday and interday assays and the percentages of RSD were all within 10.6%. The recovery ranged from 60.2 to 77.9%. The method was successfully applied to pharmacokinetic study of all three aromatic acids and one monoterpene in rat plasma. Furthermore, we compared the pharmacokinetics profile of the four compounds in normal and primary dysmenorrhea rats' plasma following oral administration of Shaofu Zhuyu decoction (SFZYD) and its ethanol supernatant extract (SFE).

Network-Based Biomarkers for Cold Coagulation Blood Stasis Syndrome and the Therapeutic Effects of Shaofu Zhuyu Decoction in Rats

“…Based on our previous studies [23, 24], the ten compounds absorbed into blood were selected to predict the biological targets. The mol2 files of ten compounds are imported into PharmMapper database (http://59.78.96.61/pharmmapper/) to predict the targets, then the target numbers were entered into the database (http://www.uniprot.org/, http://bioinfo.capitalbio.com/mas3/),  and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database (http://www.genome.jp/kegg/) was used to annotate and analyze the pathway.…”

“…Urine metabolic profile changes after days 0, 1, 3, 5, 7, 8, and 10 of SFZYD treatment, as analyzed in positive and negative ion modes ((a) ES + mode; (b) ES − mode) [23]. …”

Microcrystalline Preparation of Akebia Saponin D for its Bioavailability Enhancement in Rats

“…The area under the ASD plasma concentration-time curve from 0 to 20 h (AUC 0À20h ) and mean residence time (MRT) were calculated using noncompartmental analysis (BAPP, Version 2.0, Centre of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Republic of China). The maximal plasma concentration of drug (C max ) and the time to reach maximum plasma concentration ðT max Þ were directly obtained from plasma data (Gu et al, 2013;Su et al, 2013). In order to compare the absorption of different polymorphs in vivo, the rats were administered with capsules, but other experimental procedures were consistent with the literature .…”

Effects of Smilaxchinoside A and Smilaxchinoside C, Two Steroidal Glycosides from Smilax riparia, on Hyperuricemia in a Mouse Model

“…Urine samples were centrifuged at 3000 rpm for 10 min to remove the particulate contaminants, and supernatant was used for analysis. The serum and urine were stored at À20°C until assayed (Su et al, 2013). The levels of XOD activities, UA, Cr, and BUN in the liver and kidney were determined by the colorimetric method using commercially available kits (purchased from Beijing Aoboseng Bioengineering Institute, China), according to the manufacturers' instructions.…”