Network-Based Biomarkers for Cold Coagulation Blood Stasis Syndrome and the Therapeutic Effects of Shaofu Zhuyu Decoction in Rats

Abstract

In this study, the reverse docking methodology was applied to predict the action targets and pathways of Shaofu Zhuyu decoction (SFZYD) bioactive ingredients. Furthermore, Traditional Chinese Medicine (TCM) cold coagulation blood stasis (CCBS) syndrome was induced in female Sprague-Dawley rats with an ice-water bath and epinephrine, and SFZYD was used to treat CCBS syndrome. A metabolomic approach was used to evaluate changes in the metabolic profiles and to analyze the pharmacological mechanism of SFZYD actions. Twenty-three potential protein targets and 15 pathways were discovered, respectively; among these, pathways are associated with inflammation and immunological stress, hormone metabolism, coagulation function, and glycometabolism. There were also changes in the levels of endogenous metabolites of LysoPCs and glucuronides. Twenty endogenous metabolites were identified. Furthermore, the relative quantities of 6 endogenous metabolites in the plasma and 5 in the urine were significantly affected by SFZYD (P < 0.05). The pharmacological mechanism of SFZYD was partially associated with glycerophospholipid metabolism and pentose and glucuronate interconversions. In conclusion, our findings demonstrated that TCM CCBS pattern induced by ice water and epinephrine was complex and related to multiple metabolic pathways. SFZYD did regulate the TCM CCBS by multitargets, and biomarkers and SFZYD should be used for the clinical treatment of CCBS syndrome.

Metabolomics study on promoting blood circulation and ameliorating blood stasis: Investigating the mechanism of Angelica sinensis and its processed products

“…Blood stasis syndrome (BSS) is a common Traditional Chinese Medicine (TCM) syndrome, mainly caused by Qi deficiency, Qi stagnation, phlegm, etc. (He et al, ; Li et al, ; Lu, Xu, Zhao, & Li, ; Su et al, ). It often appears in the development and progression of many diseases, such as coronary heart disease (Ma et al, ; Wang, Li, et al, ), chronic heart failure (Gao, Zhao, et al, ) and atherosclerosis (Bai & Song, ), and seriously threatens the health and life of patients.…”

Identification of blood-activating components from Xueshuan Xinmaining Tablet based on the spectrum–effect relationship and network pharmacology analysis

“…The predicted components related to the blood-activating activity of XXT were identied with reference standards. The anticoagulant activity of the predicted components of different concentrations (10,20, and 40 mM) on normal rat plasma was determined by the method described in Section 2.3.3. 33,34 The bioactive components were nally screened out with this in vitro test.…”

“…On day 7, 30 minutes aer intragastric administration, the ii-xiii groups were injected subcutaneously with 0.1% adrenaline (1 mg kg À1 ) twice to establish an acute blood stasis model. 20 The rst dose was 1 mg kg À1 , and the second dose was reduced to 0.8 mg kg À1 at an interval of 4 hours. Two hours aer the rst dose of adrenaline, the rats in the ii-xiii groups were also immersed in an ice bath for 5 min.…”

Exploring the pharmacological effects and potential targets of paeoniflorin on the endometriosis of cold coagulation and blood stasis model rats by ultra-performance liquid chromatography tandem mass spectrometry with a pattern recognition approach

“…1 As one of the syndromes of blood stasis, cold coagulation is caused by coldevil. In traditional Chinese medicine theories, cold leads to blood clotting, and blood clotting leads to blood stasis; therefore, cold-evil easily leads to the syndrome of blood stasis, 2,3 which has a serious inuence on females during the menstrual period; moreover, it can cause various problems in women in the reproductive age. In addition, endometriosis is a grim challenge that may lead to female infertility.…”