Detection of catecholamines in single specimens of groundwater amphipods

Pfister, Gerd; Rieb, Julia; Avramov, Maria; Rock, Theresa M.; Griebler, Christian; Schramm, Karl‐Werner

doi:10.1007/s00216-013-6952-8

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…Eisenhofer, Peitzsch, and McWhinney () describe the advantages and disadvantages of ECD and MS system. MS is able to detect more analytes; however, in our laboratory the MS system could not achieve a high sensitivity for the catecholamines and a derivatization process would be needed for the analysis (Pfister et al, ). ECD is also much more specific for the monoamines than MS detectors.…”

Section: Introductionmentioning

confidence: 99%

“…Monoamines (MAs), including epinephrine (E), norepinephrine (NE), dopamine (DA) and serotonin (5-HT), play a critical role in the functioning of living organisms. They are abundant in plants (Kulma & Szopa, 2007), small crustaceans (Pfister et al, 2013) and mammals (Palkovits & Brownstein, 1989). In the brain, MAs act as neurotransmitters and control behavior such as motivation (Morita, Morishima, Sakai, & Kawaguchi, 2013), aggression (Volavka, Bilder, & Nolan, 2004) and appetite (Jason, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive analysis of nine monoamines and metabolites in small amounts of peripheral murine (C57Bl/6 J) tissues

Nagler

Schriever

Angelis

et al. 2017

Biomedical Chromatography

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Monoamines, acting as hormones and neurotransmitters, play a critical role in multiple physiological processes ranging from cognitive function and mood to sympathetic nervous system activity, fight-or-flight response and glucose homeostasis. In addition to brain and blood, monoamines are abundant in several tissues, and dysfunction in their synthesis or signaling is associated with various pathological conditions. It was our goal to develop a method to detect these compounds in peripheral murine tissues. In this study, we employed a high-performance liquid chromatography method using electrochemical detection that allows not only detection of catecholamines but also a detailed analysis of nine monoamines and metabolites in murine tissues. Simple tissue extraction procedures were optimized for muscle (gastrocnemius, extensor digitorum longus and soleus), liver, pancreas and white adipose tissue in the range of weight 10-200 mg. The system allowed a limit of detection between 0.625 and 2.5 pg μL for monoamine analytes and their metabolites, including dopamine, 3,4-dihydroxyphenylacetic acid, 3-methoxytyramine, homovanillic acid, norepinephrine, epinephrine, 3-methoxy-4-hydroxyphenylglycol, serotonin and 5-hydroxyindoleacetic acid. Typical concentrations for different monoamines and their metabolization products in these tissues are presented for C57Bl/6 J mice fed a high-fat diet.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of nine monoamines and metabolites in small amounts of peripheral murine (C57Bl/6 J) tissues

Nagler

Schriever

Angelis

et al. 2017

Biomedical Chromatography

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“…Lack of dopamine levels in the body can cause Parkinson,s disease, highly altered levels of dopamine activity cause schizophrenia . On the other hand, there are several methods reported in the literature for the quantification of DA comprising voltammetry , UHPLC , HPLC , GC , capillary electrophoresis , fluorimetry , electrochemiluminescence , all of these methods are of highly costly, time consuming and involve derivatization steps. Biosensors and potentiometric sensors have also been reported.…”

Section: Introductionmentioning

confidence: 99%

Recognition and Quantification of Some Monoamines Neurotransmitters

Elmosallamy

Saber

2016

Electroanalysis

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1IntroductionNeurotransmitters are biochemical substancest hat are produced and secreted by an euron and then diffused across as ynapse to cause excitation or inhibition of other neurons. Dopamine,s erotonin, and epinephrine are exampleso fn eurotransmitters.D opamine (DA), 4-(2-aminoethyl) benzene-1,2-diol ( Fig.1)i sa no rganicc ompound of the catechol amine that plays important roles in the human brain and body.T he brain comprises several distinct dopamine systems, one of which plays an important role in reward-motivated behavior.M ost types of reward increase the level of dopamine in the brain.O ther brain dopamine systems control the release of various hormones and involvem otor control. Several diseases of the nervouss ystema re due to the dysfunctionso ft he dopamine system. Lacko fd opaminel evels in the body can cause Parkinson,s disease,h ighly altered levels of dopamine activity cause schizophrenia [1].O nt he otherh and, there are severalm ethods reported in the literature for the quantification of DA comprising voltammetry [2-6], UHPLC[ 7-10],H PLC [11-13],G C[ 14],c apillary electrophoresis [15-17],f luorimetry[ 18-22],e lectrochemiluminescence [23, 24],a ll of these methods are of highly costly,t ime consuming and involve derivatization steps. Biosensors [25] and potentiometric sensors [26-29] have also been reported.Serotonin, 3-(2-aminoethyl) indol-5-ol, or 5-hydroxytryptamine (5-HT) ( Fig.1)i samonoamine neurotransmitter.I ti sp rimarily found in the gastrointestinal tract, blood platelets and the centraln ervous system of animal including humans.5 -HT is popularly thoughtt obe ac on-tributor to feelingso fw ell-being and happiness [ 30].T he most widely used methods for the quantification of serotonin are UHPLC [ 7,8],H PLC [31-33],G C[ 14],c apillary electrophoresis [15],v oltammetry [6,[34][35]and fluorimetry [36].S of ar,t here is no potentiometric sensorf or quantificationo fs erotonin in the literature.Epinephrine known also as adrenaline,( R)-4-(1-hydroxy-2-(methylamino) ethyl) benzene-1,2-diol( Fig. 1) is ah ormone and an eurotransmitter [37],e pinephrine and norepinephrine are separate but related hormones secreted by the medulla of the adrenal glands.T he word adrenalinei su sed in commonp arlancet od enotei ncreaseda ctivation of the sympathetics ystem associated with the energy and excitement of the fight-or-fight response [38]. Ther eported methodsf or the determination of adrenaline are voltammetry [ 5,39],U PLC [7,8,10],H PLC [13,12],c apillary electrophoresis [15,17] and fluorimetry [22].A lso therei sn op otentiometric sensor for the deter-Abstract:T hree neurotransmitter sensors for dopamine, serotonin, and epinephrine have been developed. Potentiometric sensors for serotonin and epinephrinea re novel and first to be introduced. Then eurotransmitter potentiometric sensors are based on using dibenzo-30-crown-10 (DB30C10), dibenzo-24-crown-8 (DB24C8), and dibenzo-18-crown-6 (DB18C6) as ionophores and neutral carriers incorporating in poly(vinyl chloride) matrix membrane plastici...

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