Determination of dobutamine hydrochloride by enzymatic catalytic spectrofluorimetry

Abstract

A highly sensitive and simple spectrofluorimetric method for the determination of dobutamine hydrochloride based on its inhibitory effect on the hemoglobin-catalyzed reaction of H2 O2 and l-tyrosine was developed. The relationship between the concentration of dobutamine hydrochloride and the fluorescence quenching (ΔF) of the system is linear under the optimal experimental conditions. The calibration graph is linear in the range 2.00 × 10(-7) to 3.00 × 10(-6) g/mL with a limit of detection of 4.83 × 10(-9) g/mL. This method can be used for the determination of dobutamine hydrochloride in its pharmaceutical formulations and in urine with satisfactory results.

Synthesis and Characterization of GO/ZIF-67 Nanocomposite: Investigation of Catalytic Activity for the Determination of Epinine in the Presence of Dobutamine

“…Many reports are mentioned in the literature for the determination of epinine or dobutamine using various analytical methods, including liquid chromatography, normal Raman spectroscopy, spectrophotometry, spectrofluorimetry, capillary electrophoresis, and chemiluminescence [16][17][18][19][20][21][22][23][24][25][26].…”

The theoretical description for the electrochemical determination of 4-4´-dihydroxyazobenzene, assisted by a composite of squaraine dye with cobalt (iii) oxyhydroxide in pair with cobalt (iv) oxide

“…As for now, no chemical or electrochemical methods, capable of determining accurately the 4-4´dihydroxyazobenzene have been described. Nevertheless, from the chemical composition of the compound, it is possible to foresee that, as other phenolic and hydroquinone compounds, it will be electrochemically active [9][10][11][12][13][14] for either cathodic or anodic determination. Its behavior has to be similar to that of either Sudan dyes or hydroquinone compounds.…”

Electrochemical sensing of dobutamine, paracetamol, amlodipine, and daclatasvir in serum based on thiourea SAMs over nano-gold particles–CNTs composite

“…16,24 Therefore, DB is used largely as an antimutagen in clinical treatment, a cardio stimulator, anticarcinogen, and antioxidant. [13][14][15][25][26][27][28] Psychiatric disorders and neurological diseases are associated with abnormalities of DB levels in the brain. 19 Therefore, it is important to develop a sensitive approach to determine the concentrations of DB in biological fluids.…”