Comparison of Harvest and Processing Techniques for Fat Grafting and Adipose Stem Cell Isolation

Abstract

Ultrasound- and suction-assisted lipoaspiration yielded similar retention of fat grafts in a xenograft model. Processing with cotton gauze rolling may be best suited for grafting cosmetically sensitive areas of the body in which optimal retention is critical and lower total graft volumes are needed. Filtration and centrifugation both effectively removed fluid fractions and resulted in comparable graft retention, and are more feasible when larger volumes are required.

Fat, Stem Cells, and Platelet-Rich Plasma

“…82,84,[92][93][94][95][96] Of these techniques, only Telfa rolling has any evidence of improved retention over centrifugation, based on studies conducted in the nude mouse xenograft model. 84,97,98 However, the lack of human clinical trials also limits the generalizability of these findings.…”

“…[81][82][83] Similarly, no differences have been seen in animal studies of ultrasound-assisted harvest. 84 No significant human or animal trials exist for mechanical or laser-assisted liposuction, but in vitro studies have revealed a lower stem cell yield and poorer ASC proliferation. 85,86 Preliminary in vitro and rodent studies with water-jet-assisted liposuction suggest that it improves ASC content, results in more viable adipocytes, and increases volume retention of grafts.…”

Autologous Fat Grafting to the Breast Using REVOLVE System to Reduce Clinical Costs

“…Recent literature by Rubin and colleagues has suggested that the quality of fat and its in vivo result are fairly equally comparable between washing/filtration methods and centrifugation methods. 12 Additionally, there is variability of operating room charges between different hospitals. The cost of our flagship hospital where the majority of these cases were performed may have higher charges in comparison to other hospitals.…”

Particle size in fat graft retention: A review on the impact of harvesting technique in lipofilling surgical outcomes

“…35 The adipose tissue was collected using an inline TissuTrans Filtron canister with filter pore sizes of 500 and 800 um. Unfiltered and filtered sample of the material remaining in the filter after removal of the filtrate was collected.…”