Impact of arachidonic acid enrichment of live rotifer prey on bacterial communities in rotifer and larval fish cultures

Seychelles, Laurent; Doiron, Kim; Audet, Céline; Tremblay, Réjean; Lemarchand, Karine

doi:10.1139/cjm-2012-0564

Cited by 9 publications

(7 citation statements)

References 37 publications

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“…Triplicate 4 mL aliquots of seawater samples for challenge test experiments were fixed in the dark for 15 min using 2% glutaraldehyde (final concentration; pH 7), and kept frozen at -80 • C until analysis. The total free bacteria were enumerated using an EPICS ALTRA cell sorting flow cytometer (Beckman-Coulter Inc., Mississauga, ON, Canada) equipped with a 488 nm blue laser operating at 1 mW and running at a flow rate of 60 mL min −1 as described in Seychelles et al (2013). Heterotrophic bacteria were stained (SYBR Green I, Molecular Probes Inc., Eugene, OR, USA) and subpopulations with low nucleic acid (LNA) or high nucleic acid (HNA) content were discriminated according to Belzile et al (2008).…”

Section: Bacterial Analysismentioning

confidence: 99%

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Prophylactic effect ofHaslea ostreariaculture supernatant containing the pigment marennine to stabilize bivalve hatchery production

Turcotte

Mouget

Genard

et al. 2016

Aquat. Living Resour.

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This paper explores the possibility of using the supernatant of Haslea ostrearia culture containing marennine, a natural microalgal pigment, as an antimicrobial in bivalve hatcheries. The blue mussel Mytilus edulis and the scallop Placopecten magellanicus were used as model animals, and the pathogenic marine bacteria Vibrio splendidus was used to induce larval mortality. The hypothesis tested was that V. splendidus pathogenicity in larval rearings can be controlled by using marennine-containing culture supernatants. The effect of three marennine concentrations was tested on a larval rearing over 20 days for M. edulis and 9 days for P. magellanicus. At a low dose (0.1 mg L −1 ), survival and physiological condition were both higher than in the control. In bacterial challenges, larvae were exposed to V. splendidus for 72 h, with or without marennine. The bacterial challenge caused significant mortality when compared to controls, while the marennine-treated larvae showed significantly higher survival. Results show that marennine is an interesting molecule for pathogen control in hatcheries as it is active at low concentrations and significantly enhanced larval survival and physiological condition.

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Section: Bacterial Analysismentioning

confidence: 99%

“…Data analysis was performed with Expo321 software (Beckman Coulter Inc., Fullerton, BC, CA). For the purpose of this study, the percentage of HNA (%HNA being the ratio of HNA cells to total bacterial count) was used as a proxy for the physiological state of the bacterial population (Seychelles et al 2013).…”

Section: Bacterial Analysismentioning

confidence: 99%

Prophylactic effect ofHaslea ostreariaculture supernatant containing the pigment marennine to stabilize bivalve hatchery production

Turcotte

Mouget

Genard

et al. 2016

Aquat. Living Resour.

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“…The lipid composition of larvae and/or juveniles has been presented in detail for Florida pompano (Cavalin and Weirich, 2009;Hauville et al, 2014Hauville et al, , 2016Rombenso et al, 2016Rombenso et al, , 2017 Jackson et al, 2020) and for winter flounder (Mercier et al, 2004;Seychelles et al, 2011Seychelles et al, , 2013Vagner et al, 2013;Bélanger et al, 2018;Martinez-Silva et al, 2018). While these studies examined different developmental stages, we have merged the results for the purpose of comparison.…”

Section: Introductionmentioning

confidence: 99%

Essential Fatty Acid Requirements in Tropical and Cold-Water Marine Fish Larvae and Juveniles

et al. 2021

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To improve survival at early developmental stages (larvae and juveniles) of captive fish species, essential nutrients [i.e., essential fatty acids (EFA)] need to be identified. The physiological needs are likely to be different among species, particularly among those using different thermal habitats, because lipids are largely used to maintain cell membrane integrity (homeoviscous adaptation) in fishes. This review paper will focus on currently published research and the main results from our laboratories regarding optimum qualitative EFA requirements during larval and early juvenile stages in a warm-water marine species, the Florida pompano (Trachinotus carolinus), and a cold-water marine species, the winter flounder (Pseudopleuronectes americanus). To identify the qualitative optimal EFA requirements, we calculated the ratio of certain fatty acids (FA) in larval or early juvenile tissues to total FA present in the diet. This ratio indicates whether a specific FA from prey is selectively incorporated by larvae and juveniles. Overall, we found that young larvae from both cold- and warm-water species have greater demands for n-3 and n-6 highly unsaturated fatty acids (HUFA) than do larvae at weaning stages. However, the qualitative EFA requirements of the cold-water species at all early developmental stages were higher than those of the warm-water species. Enriched rotifer diets provided satisfactory amounts of omega 3 and omega 6 in Florida pompano, with small selective retention for docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and arachidonic acid (ARA), suggesting a potential minor diet deficiency in these EFA. There were higher deficiencies in the cold-water species fed enriched rotifers, as demonstrated by the higher selective retentions of all EFA (DHA, EPA, and ARA), with the exception of larvae fed with copepods. The physiological needs in EFA for juvenile development seemed to be better met for both species when they were fed micro pellets. From the beginning of settlement and in young juveniles, qualitative values of 12% DHA, 10% EPA, 5% ARA, and 40% PUFA of total FA seem to be required for winter flounder juvenile development. In Florida pompano, these requirements could be met until larger juvenile stages, with 15% DHA, 3% EPA, 2% ARA, 2% DPA, and total PUFA below 30% of total FA. This review was done to aid future research aiming to develop nutritionally balanced microdiets or live-prey enrichment diets to satisfy the physiological requirements of captive tropical and cold-water marine fish species.

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“…[11][12][13] Additionally, some studies showed that arachidonic acid, another essential unsaturated fatty acid, has a protective role against the reduction of other fatty acids and also it preserves physiological mechanisms in animal models. [14][15][16] Besides, essential unsaturated fatty acids can increase the anti-oxidative activity of catalase which is an essential antioxidant enzyme for protecting cells against damages. 8,9 Catalase can convert hydrogen peroxide to oxygen and water.…”

Section: Introductionmentioning

confidence: 99%

Effect of Essential Unsaturated Fatty Acids on Structure and Function of Catalase at High Glucose Concentration

Mirmiranpoor¹,

Hashemi²,

Firouzabadi³

et al. 2017

Int J Enteric Pathog

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Impact of arachidonic acid enrichment of live rotifer prey on bacterial communities in rotifer and larval fish cultures

Cited by 9 publications

References 37 publications

Prophylactic effect ofHaslea ostreariaculture supernatant containing the pigment marennine to stabilize bivalve hatchery production

Prophylactic effect ofHaslea ostreariaculture supernatant containing the pigment marennine to stabilize bivalve hatchery production

Essential Fatty Acid Requirements in Tropical and Cold-Water Marine Fish Larvae and Juveniles

Effect of Essential Unsaturated Fatty Acids on Structure and Function of Catalase at High Glucose Concentration

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