The similarity between human embryonic stem cell-derived epithelial cells and ameloblast-lineage cells

Zheng, Liwei; Linthicum, Logan; DenBesten, Pamela; Zhang, Yan

doi:10.1038/ijos.2013.14

Cited by 18 publications

(20 citation statements)

References 35 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunocytochemical studies reveal that HAT-7 cells exhibit several ameloblast characteristics, including the expression of ameloblastin and amelogenin (Kawano et al, 2002). Additionally, expression profiling has shown maturation-stage ameloblast marker proteins such as amelotin and kallikrein-4 (Klk4) are also expressed in these cells (Harada et al, 2006;Matsumoto, Harada, Saito, & Taniguchi, 2011;Yoshizaki et al, 2008;Zheng, Linthicum, DenBesten, & Zhang, 2013).…”

Section: The Hat-7 Cell Model To Functionally Study Ph Regulation Bmentioning

confidence: 99%

Importance of bicarbonate transport in pH control during amelogenesis — need for functional studies

et al. 2017

View full text Add to dashboard Cite

Dental enamel, the hardest mammalian tissue, is produced by ameloblasts. Ameloblasts show many similarities to other transporting epithelia although their secretory product, the enamel matrix, is quite different. Ameloblasts direct the formation of hydroxyapatite crystals, which liberate large quantities of protons that then need to be buffered to allow mineralization to proceed. Buffering requires a tight pH regulation and secretion of bicarbonate by ameloblasts. Many investigations have used immunohistochemical and knockout studies to determine the effects of these genes on enamel formation, but up till recently very little functional data were available for mineral ion transport. To address this, we developed a novel 2D in vitro model using HAT-7 ameloblast cells. HAT-7 cells can be polarized and develop functional tight junctions. Furthermore, they are able to accumulate bicarbonate ions from the basolateral to the apical fluid spaces. We propose that in the future, the HAT-7 2D system along with similar cellular models will be useful to functionally model ion transport processes during amelogenesis. Additionally, we also suggest that similar approaches will allow a better understanding of the regulation of the cycling process in maturation-stage ameloblasts, and the pH sensory mechanisms, which are required to develop sound, healthy enamel. K E Y W O R D Sameloblast, amelogenesis, bicarbonate, enamel, functional model, HAT-7, in vitro, ion transport, microfluorometry, pH regulation, transepithelial resistance, transwell

show abstract

Section: The Hat-7 Cell Model To Functionally Study Ph Regulation Bmentioning

confidence: 99%

Importance of bicarbonate transport in pH control during amelogenesis — need for functional studies

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Human fetal OEs and ALCs were cultured in order to investigate the function of miR-224 in human epithelial cells in vitro. OEs and ALCs are derived from the same progenitor cell source, but OE is not secretory epithelium (12). In the present study, the miR-224 mimic and inhibitor were transfected into either cultured ALCs or OEs.…”

Section: Resultsmentioning

confidence: 99%

“…Human fetal oral buccal mucosal epithelial cells (OEs) and ALCs were cultured as described previously (12) in KGM-2 medium (Lonza, Basel, Switzerland) with 1% penicillin/streptomycin, and 0.05 mmol/liter calcium on BD Primaria tissue culture dishes (BD Biosciences, Franklin Lakes, NJ). Clonally selected cells were grown at either pH 7.2 with 0, 10, and 50 M fluoride or at pH 6.2 without fluoride for 72 h.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA 224 Regulates Ion Transporter Expression in Ameloblasts To Coordinate Enamel Mineralization

Fan

Zhou

et al. 2015

Molecular and Cellular Biology

View full text Add to dashboard Cite

Enamel mineralization is accompanied by the release of protons into the extracellular matrix, which is buffered to regulate the pH value in the local microenvironment. The present study aimed to investigate the role of microRNA 224 (miR-224) as a regulator of SLC4A4 and CFTR, encoding the key buffering ion transporters, in modulating enamel mineralization. miR-224 was significantly downregulated as ameloblasts differentiated, in parallel with upregulation of SLC4A4 and CFTR. Overexpression of miR-224 downregulated SLC4A4 and CFTR expression in cultured human epithelial cells. A microRNA luciferase assay confirmed the specific binding of miR-224 to the 3= untranslated regions (UTRs) of SLC4A4 and CFTR mRNAs, thereby inhibiting protein translation. miR-224 agomir injection in mouse neonatal incisors resulted in normal enamel length and thickness, but with disturbed organization of the prism structure and deficient crystal growth. Moreover, the enamel Ca/P ratio and microhardness were markedly reduced after miR-224 agomir administration. These results demonstrate that miR-224 plays a pivotal role in fine tuning enamel mineralization by modulating SLC4A4 and CFTR to maintain pH homeostasis and support enamel mineralization.

show abstract

“…Still, only a small percentage of these explants in subrenal cultures demonstrated the formation of dental enamel [60]. Embryonic stem/progenitor cells were similarly demonstrated to differentiate into oral ectoderm and dental epithelium, using variable concentrations of BMP-4 [61]. The formed dental epithelium, when mixed with mouse embryonic dental mesenchyme and transplanted into renal capsules for thirty days, subsequently generated teeth-like structures, including dentin and enamel, with an incisor-like appearance [28].…”

Section: Enamel Regenerationmentioning

confidence: 99%

Tissue Engineering Approaches for Enamel, Dentin, and Pulp Regeneration: An Update

Ahmed

Abouauf

AbuBakr

et al. 2020

Stem Cells International

View full text Add to dashboard Cite

Stem/progenitor cells are undifferentiated cells characterized by their exclusive ability for self-renewal and multilineage differentiation potential. In recent years, researchers and investigations explored the prospect of employing stem/progenitor cell therapy in regenerative medicine, especially stem/progenitor cells originating from the oral tissues. In this context, the regeneration of the lost dental tissues including enamel, dentin, and the dental pulp are pivotal targets for stem/progenitor cell therapy. The present review elaborates on the different sources of stem/progenitor cells and their potential clinical applications to regenerate enamel, dentin, and the dental pulpal tissues.

show abstract

The similarity between human embryonic stem cell-derived epithelial cells and ameloblast-lineage cells

Cited by 18 publications

References 35 publications

Importance of bicarbonate transport in pH control during amelogenesis — need for functional studies

Importance of bicarbonate transport in pH control during amelogenesis — need for functional studies

MicroRNA 224 Regulates Ion Transporter Expression in Ameloblasts To Coordinate Enamel Mineralization

Tissue Engineering Approaches for Enamel, Dentin, and Pulp Regeneration: An Update

Contact Info

Product

Resources

About