Expression status of mRNA for sex hormone receptors in human dental pulp cells and the response to sex hormones in the cells

Inaba, T.; Kobayashi, Tomoko; Tsutsui, Takeo W.; Ogawa, Masaaki; Uchida, Minoru; Tsutsui, Takeki

doi:10.1016/j.archoralbio.2013.02.001

Cited by 13 publications

(14 citation statements)

References 28 publications

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“…Our observation of ER expression in DPCs confirms previous reports of expression in these cells [21,22]. Although we report for the first time up regulation of both ER 1 and ER 2 in DPCs during osteogenic differentiation, increased expression of ER during osteogenic differentiation of closely related periodontal ligament stem cells (PDLCs) has been described previously [38].…”

Section: Discussionsupporting

confidence: 92%

“…[11,12] Several tissues within the oral cavity contain populations of mesenchymal stem cells (MSCs) [13][14][15][16] which are able to differentiate into a matrix mineralising phenotype and this can be stimulated by the action of E 2 [17,18]. Accordingly, ER mRNA and protein is expressed in niche MSC populations in the oral cavity including those from periodontal ligament-PDLCs- [19,20] and dental pulp-DPCs [21,22] although the proteins remain rather poorly characterised in these tissues. Two classical ER isoforms have been described-ER and ER -which are structurally and functionally related but which represent two different gene products [23,24].…”

Section: Introductionmentioning

confidence: 99%

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Oestrogen receptor β (ERβ) regulates osteogenic differentiation of human dental pulp cells

Alhodhodi

Alkharobi

Humphries

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

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Estradiol (E) has many important actions in the tissues of the oral cavity. Disruption of E metabolism or alterations in systemic E concentrations have been associated with compromised periodontal health. In many instances such changes occur secondarily to the well characterised effects of E on bone physiology -especially maintenance of bone mineral density (BMD). Despite these important epidemiological findings, little is known about the mechanism of action of E in oral tissues or the expression and function of oestrogen receptor (ER) isoforms in these tissues. We have isolated human dental pulp cells (hDPCs), which are able to differentiate towards an osteogenic lineage under appropriate culture conditions. We show that hDPCs express ERα, ERβ1, ERβ2 and the cell membrane associated G protein-coupled ER (GPR30). Following osteogenic differentiation of hDPCs, ERβ1 and ERβ2 were up regulated approximately 50-fold while ERα and GPR30 were down regulated, but to a much lesser degree (approximately 2-fold). ERβ was characterised as a 59kDa protein following Western blot analysis with validated antibodies and ERβ was detected in both nuclear and cytoplasmic cell compartments following immunofluorescence (IF) and immunohistochemical (IHC) analysis of cultured cells. Furthermore isoform specific antibodies detected both ERβ1 and ERβ2 in DPC cultures and in situ analysis of ERβ expression in decalcified tooth/pulp sections identified the odontoblast layer of pulp cells juxtaposed to the tooth enamel as strongly reactive for both ERβ isoforms. Finally the use of isoform specific agonists identified ERβ as the main receptor responsible for the pro-osteogenic effect of oestrogenic hormones in this tissue. Our data suggest that oestrogens stimulated osteogenic differentiation in hDPCs and that this action is mediated principally through the ERβ isoform. These findings may have important consequences for the investigation and treatment of oral and periodontal pathologies which are associated with imbalances in oestrogen concentrations and action.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Oestrogen receptor β (ERβ) regulates osteogenic differentiation of human dental pulp cells

Alhodhodi

Alkharobi

Humphries

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

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“…Corticoids affect enamel hardness and mineralization (Pawlicki et al, 1992), and a responsive element for GR (GRE) has been found in the amelogenin promoter (Gibson et al, 1997). Concerning the AR, it has already been detected in dental pulp cells (Dale et al, 2002; Inaba et al, 2013). In addition, our past work, as well as the present study, show that AR expression in the maturation-stage ameloblasts which is higher than in the secretion-stage and mesenchymal cells, suggesting a selective role of androgens in enamel final mineralization (Jedeon et al, 2016b).…”

Section: Discussionmentioning

confidence: 96%

Expression of Steroid Receptors in Ameloblasts during Amelogenesis in Rat Incisors

et al. 2016

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Endocrine disrupting chemicals (EDCs) play a part in the modern burst of diseases and interfere with the steroid hormone axis. Bisphenol A (BPA), one of the most active and widely used EDCs, affects ameloblast functions, leading to an enamel hypomineralization pattern similar to that of Molar Incisor Hypomineralization (MIH). In order to explore the molecular pathways stimulated by BPA during amelogenesis, we thoroughly investigated the receptors known to directly or indirectly mediate the effects of BPA. The expression patterns of high affinity BPA receptors (ERRγ, GPR30), of ketosteroid receptors (ERs, AR, PGR, GR, MR), of the retinoid receptor RXRα, and PPARγ were established using RT-qPCR analysis of RNAs extracted from microdissected enamel organ of adult rats. Their expression was dependent on the stage of ameloblast differentiation, except that of ERβ and PPARγ which remained undetectable. An additional large scale microarray analysis revealed three main groups of receptors according to their level of expression in maturation-stage ameloblasts. The expression level of RXRα was the highest, similar to the vitamin D receptor (VDR), whereas the others were 13 to 612-fold lower, with AR and GR being intermediate. Immunofluorescent analysis of VDR, ERα and AR confirmed their presence mainly in maturation- stage ameloblasts. These data provide further evidence that ameloblasts express a specific combination of hormonal receptors depending on their developmental stage. This study represents the first step toward understanding dental endocrinology as well as some of the effects of EDCs on the pathophysiology of amelogenesis.

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“…Researchers have also utilized hormones as differentiation inducers. One such example includes estrogen hormone that regulates cell proliferation and differentiation [15,16]. Interestingly, 17ß-estradiol (E2)-supplementation has been found to enhance the odonto/osteoblast differentiation capacity of MSCs by regulating estrogen receptor-α (ER-α), c-Src, and mitogen-activated protein kinases (MAPKs) [15].…”

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confidence: 99%

Evaluation of odonto/osteogenic differentiation potential from different regions derived dental tissue stem cells and effect of 17β-estradiol on efficiency

et al. 2021

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Background The dentin is a tissue, which is formed by odontoblasts at the pulp interface of the teeth that supports the enamel. Odontoblasts, the cranial neural crest cells are derived from ectodermal mesenchymal stem cells (MSCs) and are long and polarized cells. They are present at the outer surface of dentin and play a prominent role about dentin formation. Recently, attention has been focused on induction of odontoblast using various type of MSCs and effects of the 17ß-estradiol supplementation. In this study, we establish an efficient odonto/osteoblast differentiation protocol using 17ß-estradiol supplementation while comparing the odonto/osteoblast ability of various dental MSCs. Methods Same donor derived four types of dental MSCs namely dental pulp stem cells (DPSCs), stem cells from apical papilla (SCAP), dental follicle stem cells (DFSCs), and periodontal ligament stem cells (PDLSCs) were evaluated for their stemness characteristics and potency towards odonto/osteoblast (Induced odonto/osteoblast) differentiation. Then 17ß-estradiol supplementation of 0 and 10 µM was applied to the odonto/osteoblast differentiation media for 14 days respectively. Furthermore, mRNA and protein levels of odonto/osteoblast markers were evaluated. Results All of the experimental groups displayed stemness characteristics by showing adipocyte and chondrocyte differentiation abilities, expression for cell surface markers and cell proliferation capacity without any significant differences. Moreover, all dental derived MSCs were shown to have odonto/osteoblast differentiation ability when cultured under specific conditions and also showed positive expression for odontoblast markers at both mRNA and protein level. Among all, DPSCs revealed the higher differentiation potential than other dental MSCs. Furthermore, odonto/osteoblast differentiation potential was enhanced by supplementing the differentiation media with 17ß-estradiol (E2). Conclusions Thus, DPSCs possess higher odonto/osteogenic potential than the SCAPs, DFSCs, PDLSCs and their differentiation capacity can by further enhanced under E2 supplementation.

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Expression status of mRNA for sex hormone receptors in human dental pulp cells and the response to sex hormones in the cells

Cited by 13 publications

References 28 publications

Oestrogen receptor β (ERβ) regulates osteogenic differentiation of human dental pulp cells

Oestrogen receptor β (ERβ) regulates osteogenic differentiation of human dental pulp cells

Expression of Steroid Receptors in Ameloblasts during Amelogenesis in Rat Incisors

Evaluation of odonto/osteogenic differentiation potential from different regions derived dental tissue stem cells and effect of 17β-estradiol on efficiency

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